ASR3_SARSH
ID ASR3_SARSH Reviewed; 854 AA.
AC A0A2U8U2K7;
DT 26-FEB-2020, integrated into UniProtKB/Swiss-Prot.
DT 12-SEP-2018, sequence version 1.
DT 25-MAY-2022, entry version 14.
DE RecName: Full=Transcription factor asR3 {ECO:0000303|PubMed:29773797};
DE AltName: Full=Xenovulene A biosynthesis cluster protein R3 {ECO:0000303|PubMed:29773797};
GN Name=asR3 {ECO:0000303|PubMed:29773797};
OS Sarocladium schorii (Acremonium strictum (strain IMI 501407)).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Sarocladiaceae; Sarocladium;
OC unclassified Sarocladium.
OX NCBI_TaxID=2203296;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION, AND FUNCTION.
RX PubMed=29773797; DOI=10.1038/s41467-018-04364-9;
RA Schor R., Schotte C., Wibberg D., Kalinowski J., Cox R.J.;
RT "Three previously unrecognised classes of biosynthetic enzymes revealed
RT during the production of xenovulene A.";
RL Nat. Commun. 9:1963-1963(2018).
RN [2]
RP BIOTECHNOLOGY.
RX PubMed=9262310;
RA Thomas P., Sundaram H., Krishek B.J., Chazot P., Xie X., Bevan P.,
RA Brocchini S.J., Latham C.J., Charlton P., Moore M., Lewis S.J.,
RA Thornton D.M., Stephenson F.A., Smart T.G.;
RT "Regulation of neuronal and recombinant GABA(A) receptor ion channels by
RT xenovulene A, a natural product isolated from Acremonium strictum.";
RL J. Pharmacol. Exp. Ther. 282:513-520(1997).
RN [3]
RP FUNCTION.
RX PubMed=17912413; DOI=10.1039/b708614h;
RA Bailey A.M., Cox R.J., Harley K., Lazarus C.M., Simpson T.J., Skellam E.;
RT "Characterisation of 3-methylorcinaldehyde synthase (MOS) in Acremonium
RT strictum: first observation of a reductive release mechanism during
RT polyketide biosynthesis.";
RL Chem. Commun. (Camb.) 39:4053-4055(2007).
RN [4]
RP FUNCTION.
RX PubMed=20552126; DOI=10.1039/c0cc01162b;
RA Fisch K.M., Skellam E., Ivison D., Cox R.J., Bailey A.M., Lazarus C.M.,
RA Simpson T.J.;
RT "Catalytic role of the C-terminal domains of a fungal non-reducing
RT polyketide synthase.";
RL Chem. Commun. (Camb.) 46:5331-5333(2010).
CC -!- FUNCTION: Transcription factor; part of the gene cluster that mediates
CC the biosynthesis of xenovulene A, an unusual meroterpenoid that has
CC potent inhibitory effects on the human gamma-aminobutyrate A (GABAA)
CC benzodiazepine receptor. {ECO:0000269|PubMed:29773797}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00227}.
CC -!- INDUCTION: Expression is significantly up-regulated under xenovulene A
CC producing condition. {ECO:0000269|PubMed:29773797}.
CC -!- BIOTECHNOLOGY: Xenovulene A is a natural product exhibiting little
CC structural resemblance with classical benzodiazepines yet is able to
CC displace high-affinity ligand binding to the benzodiazepine site of the
CC gamma-aminobutyrate A (GABAA) receptor and could be potentially used as
CC an anti-depressant with reduced addictive properties.
CC {ECO:0000269|PubMed:9262310}.
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DR EMBL; MG736817; AWM95792.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2U8U2K7; -.
DR SMR; A0A2U8U2K7; -.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR CDD; cd00067; GAL4; 1.
DR Gene3D; 4.10.240.10; -; 1.
DR InterPro; IPR007219; Transcription_factor_dom_fun.
DR InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR Pfam; PF04082; Fungal_trans; 1.
DR Pfam; PF00172; Zn_clus; 1.
DR SMART; SM00066; GAL4; 1.
DR SUPFAM; SSF57701; SSF57701; 1.
DR PROSITE; PS00463; ZN2_CY6_FUNGAL_1; 1.
DR PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Metal-binding; Nucleus; Transcription;
KW Transcription regulation; Zinc.
FT CHAIN 1..854
FT /note="Transcription factor asR3"
FT /id="PRO_0000449189"
FT DNA_BIND 19..45
FT /note="Zn(2)-C6 fungal-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
FT REGION 56..156
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 74..89
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 121..156
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 854 AA; 93924 MW; 09C669F3E80C29C6 CRC64;
MDKQQSPPPR RRRRPMLSCW ECRRRKIKCD RNDPCAHCIR HETQCVFTAH TGTVATDSDV
SRTRPGPKSA SRPGVSIASG SNSAVATRPS SDAGRGGSAI PTAPPPRGPS SLPSIAGPNH
PFNILNPSQD VSAPSTQVLQ PSSRGPSLST NTSPSASSAV IHTLLERIKT LEDSYSSLSS
RHGDSIESRV SSIGEPRDLR PAWEDTVARH ANAARRPAGL AGNWQDIVRR KSTKLGRSHR
MGDAPDFAAI IECYSVMMGL TSKDDFSHIP EIKDLLAEGG GLLVESKQIA QRLKLGQPSK
PPHTLSVVPP SPKLGQYLVR PTRERADVMA KLYFESFESA YRILHAPTFW ANYEKYWQAP
EAASNAVYLQ ILLVVAIGSS IYDHGDCNAV LDNIEMSRPC IFAAENWLAD PFEKDRLEIA
GLQVYCLCNM ARQIYNVGGD LIWTSMGPLL YRALQVGLHR EPGRLPGVSM FQAEIRRRLW
ATILDMVVQA SLDALMPPMM SLDEFDVEPP ANINDEEMDE DTTIIVPHPR TTFTSTSAQL
ALLETLPLRF GIVQYLFGMQ SEQSYPSVLS LSSRLASALS MCNSLGNLGP QSQDQSHDNN
MGTTNTTMAF RSITPFQRNL LDYLVRRFMT PLHMFFATQS HSNPVFHYSL TVSLDAALAL
VSPATPTTTT ISTPAHNNEE TNHFDRLLST AGGLFREGFR TALTTISLAL LVHAKTQQVS
GTLHRAPQHR ESLKAAVRDL VALAERRVRN GDTNIRAYMF LNMVLAQVEA MEAGARAGDG
AFEMALVSGA VKSLRFCRDV IKTRAETRAP VDWEEMDFDA DGMASFLPGD MDADAFGLDW
DWESLLTTMD MAEG
