ID   ASR4_SARSH              Reviewed;         632 AA.
AC   A0A2U8U2L8;
DT   26-FEB-2020, integrated into UniProtKB/Swiss-Prot.
DT   12-SEP-2018, sequence version 1.
DT   25-MAY-2022, entry version 16.
DE   RecName: Full=Transcription factor asR4 {ECO:0000303|PubMed:29773797};
DE   AltName: Full=Xenovulene A biosynthesis cluster protein R4 {ECO:0000303|PubMed:29773797};
GN   Name=asR4 {ECO:0000303|PubMed:29773797};
OS   Sarocladium schorii (Acremonium strictum (strain IMI 501407)).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Sarocladiaceae; Sarocladium;
OC   unclassified Sarocladium.
OX   NCBI_TaxID=2203296;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION, FUNCTION, AND DISRUPTION
RP   PHENOTYPE.
RX   PubMed=29773797; DOI=10.1038/s41467-018-04364-9;
RA   Schor R., Schotte C., Wibberg D., Kalinowski J., Cox R.J.;
RT   "Three previously unrecognised classes of biosynthetic enzymes revealed
RT   during the production of xenovulene A.";
RL   Nat. Commun. 9:1963-1963(2018).
RN   [2]
RP   BIOTECHNOLOGY.
RX   PubMed=9262310;
RA   Thomas P., Sundaram H., Krishek B.J., Chazot P., Xie X., Bevan P.,
RA   Brocchini S.J., Latham C.J., Charlton P., Moore M., Lewis S.J.,
RA   Thornton D.M., Stephenson F.A., Smart T.G.;
RT   "Regulation of neuronal and recombinant GABA(A) receptor ion channels by
RT   xenovulene A, a natural product isolated from Acremonium strictum.";
RL   J. Pharmacol. Exp. Ther. 282:513-520(1997).
RN   [3]
RP   FUNCTION.
RX   PubMed=17912413; DOI=10.1039/b708614h;
RA   Bailey A.M., Cox R.J., Harley K., Lazarus C.M., Simpson T.J., Skellam E.;
RT   "Characterisation of 3-methylorcinaldehyde synthase (MOS) in Acremonium
RT   strictum: first observation of a reductive release mechanism during
RT   polyketide biosynthesis.";
RL   Chem. Commun. (Camb.) 39:4053-4055(2007).
RN   [4]
RP   FUNCTION.
RX   PubMed=20552126; DOI=10.1039/c0cc01162b;
RA   Fisch K.M., Skellam E., Ivison D., Cox R.J., Bailey A.M., Lazarus C.M.,
RA   Simpson T.J.;
RT   "Catalytic role of the C-terminal domains of a fungal non-reducing
RT   polyketide synthase.";
RL   Chem. Commun. (Camb.) 46:5331-5333(2010).
CC   -!- FUNCTION: Transcription factor; part of the gene cluster that mediates
CC       the biosynthesis of xenovulene A, an unusual meroterpenoid that has
CC       potent inhibitory effects on the human gamma-aminobutyrate A (GABAA)
CC       benzodiazepine receptor. {ECO:0000269|PubMed:29773797}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00227}.
CC   -!- INDUCTION: Expression is significantly up-regulated under xenovulene A
CC       producing condition. {ECO:0000269|PubMed:29773797}.
CC   -!- DISRUPTION PHENOTYPE: Does not affect the production of xenovulene A.
CC       {ECO:0000269|PubMed:29773797}.
CC   -!- BIOTECHNOLOGY: Xenovulene A is a natural product exhibiting little
CC       structural resemblance with classical benzodiazepines yet is able to
CC       displace high-affinity ligand binding to the benzodiazepine site of the
CC       gamma-aminobutyrate A (GABAA) receptor and could be potentially used as
CC       an anti-depressant with reduced addictive properties.
CC       {ECO:0000269|PubMed:9262310}.
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DR   EMBL; MG736817; AWM95793.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A2U8U2L8; -.
DR   SMR; A0A2U8U2L8; -.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   CDD; cd00067; GAL4; 1.
DR   Gene3D; 4.10.240.10; -; 1.
DR   InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR   InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR   Pfam; PF00172; Zn_clus; 1.
DR   SUPFAM; SSF57701; SSF57701; 1.
DR   PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; Metal-binding; Nucleus; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..632
FT                   /note="Transcription factor asR4"
FT                   /id="PRO_0000449190"
FT   DNA_BIND        26..52
FT                   /note="Zn(2)-C6 fungal-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
FT   REGION          101..129
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          270..311
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          324..356
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   632 AA;  68987 MW;  8D39907C3DCCE889 CRC64;
     MPASLPGQDG GGTRTTRPRS QLRAACDSCN KSKTKCPGGN PCPLCQCSGI RCHYSVAHQL
     GRPKGSKNKR TLMREMQGSL GNGDASGQFQ PNRIVTTAQA HISSNPSPVG SQSQQQQHPQ
     QAGQQQQQQQ QQQQQQHQQQ QQQQQQQQQQ QQQQQQQQQQ QQQQQQQHQQ QQQQQQQQQH
     LLPHAFSNTM QNAGTTAAYA GAEKEDSPLA MDDGELFSML DQTLLMDTMT DEMSDFGQLV
     PQALPHPMTM PESLTSLLNH DTAFDATQLS NEYSTSSNSK PSTTDDSCMA LPTPDSSTTH
     RPSSSVFGDG HITFSSGTAS ASRTSASSIV EQQSIPTSPV TVAPMSPAKS SSVPRTLPPA
     TVTPTCTCLP GLVTLICQLE DLRHPPPPRH TSGQHPHQHP ASPYSLKCIL QGVQLAEEPW
     AGFARCLDAA GARKRHGAQE NTVQDDDGND HHRHALALYA MSIRIILSSI HKYRIALSMT
     GQQQRQHNFG DSPEDLDDAA SVLVSVGGVQ LAGETKSEMI KIAVRLALKQ TTAALMRLLE
     CRNRSSSAPS SATMQVAEYS VGHGRFESTA MVRDHPLPHS MSYSSLKTLA GPHTNMITGQ
     DQLEKRENVA GMLSILQYTT KDLIDGANFD WR