ID   PDSA_NEOFI              Reviewed;         738 AA.
AC   A1CVK0;
DT   22-FEB-2012, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 1.
DT   25-MAY-2022, entry version 76.
DE   RecName: Full=Protostadienol synthase A;
DE            EC=5.4.99.32;
GN   Name=pdsA; ORFNames=NFIA_101360;
OS   Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164
OS   / JCM 1740 / NRRL 181 / WB 181) (Aspergillus fischerianus).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Fumigati.
OX   NCBI_TaxID=331117;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181
RC   / WB 181;
RX   PubMed=18404212; DOI=10.1371/journal.pgen.1000046;
RA   Fedorova N.D., Khaldi N., Joardar V.S., Maiti R., Amedeo P., Anderson M.J.,
RA   Crabtree J., Silva J.C., Badger J.H., Albarraq A., Angiuoli S., Bussey H.,
RA   Bowyer P., Cotty P.J., Dyer P.S., Egan A., Galens K., Fraser-Liggett C.M.,
RA   Haas B.J., Inman J.M., Kent R., Lemieux S., Malavazi I., Orvis J.,
RA   Roemer T., Ronning C.M., Sundaram J.P., Sutton G., Turner G., Venter J.C.,
RA   White O.R., Whitty B.R., Youngman P., Wolfe K.H., Goldman G.H.,
RA   Wortman J.R., Jiang B., Denning D.W., Nierman W.C.;
RT   "Genomic islands in the pathogenic filamentous fungus Aspergillus
RT   fumigatus.";
RL   PLoS Genet. 4:E1000046-E1000046(2008).
CC   -!- FUNCTION: Protostadienol synthase which cyclizes (3S)-oxidosqualene to
CC       (17Z)-protosta-17(20),24-dien-3-beta-ol (protostadienol), the
CC       biosynthetic precursor of helvolic acid, a secondary metabolite which
CC       promotes virulence. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(S)-2,3-epoxysqualene = (17Z)-protosta-17(20),24-dien-3beta-
CC         ol; Xref=Rhea:RHEA:30987, ChEBI:CHEBI:15441, ChEBI:CHEBI:62457;
CC         EC=5.4.99.32;
CC   -!- SIMILARITY: Belongs to the terpene cyclase/mutase family.
CC       {ECO:0000305}.
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DR   EMBL; DS027685; EAW24652.1; -; Genomic_DNA.
DR   RefSeq; XP_001266549.1; XM_001266548.1.
DR   AlphaFoldDB; A1CVK0; -.
DR   SMR; A1CVK0; -.
DR   STRING; 36630.CADNFIAP00009757; -.
DR   EnsemblFungi; EAW24652; EAW24652; NFIA_101360.
DR   GeneID; 4594041; -.
DR   KEGG; nfi:NFIA_101360; -.
DR   VEuPathDB; FungiDB:NFIA_101360; -.
DR   eggNOG; KOG0497; Eukaryota.
DR   HOGENOM; CLU_009074_2_1_1; -.
DR   OMA; SMLCCWI; -.
DR   OrthoDB; 365003at2759; -.
DR   Proteomes; UP000006702; Unassembled WGS sequence.
DR   GO; GO:0005811; C:lipid droplet; IEA:InterPro.
DR   GO; GO:0016866; F:intramolecular transferase activity; IEA:InterPro.
DR   GO; GO:0006694; P:steroid biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0016104; P:triterpenoid biosynthetic process; IEA:InterPro.
DR   CDD; cd02892; SQCY_1; 1.
DR   InterPro; IPR032696; SQ_cyclase_C.
DR   InterPro; IPR032697; SQ_cyclase_N.
DR   InterPro; IPR018333; Squalene_cyclase.
DR   InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR   PANTHER; PTHR11764; PTHR11764; 1.
DR   Pfam; PF13243; SQHop_cyclase_C; 1.
DR   Pfam; PF13249; SQHop_cyclase_N; 1.
DR   SFLD; SFLDG01016; Prenyltransferase_Like_2; 1.
DR   SUPFAM; SSF48239; SSF48239; 2.
DR   TIGRFAMs; TIGR01787; squalene_cyclas; 1.
PE   3: Inferred from homology;
KW   Isomerase; Lipid biosynthesis; Lipid metabolism; Reference proteome;
KW   Repeat; Steroid biosynthesis; Virulence.
FT   CHAIN           1..738
FT                   /note="Protostadienol synthase A"
FT                   /id="PRO_0000415497"
FT   REPEAT          132..173
FT                   /note="PFTB 1"
FT   REPEAT          490..531
FT                   /note="PFTB 2"
FT   REPEAT          567..607
FT                   /note="PFTB 3"
FT   REPEAT          616..663
FT                   /note="PFTB 4"
FT   ACT_SITE        463
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:P48449"
SQ   SEQUENCE   738 AA;  83217 MW;  C36579825E6BA4AC CRC64;
     MTTDSSMPAT VIGKAEFSNT KAASEFGTNL SRWRLNVDNG RHMWEYLESE DEARKRPQSI
     LEKYWLGLPY ELPARPRATR ALEAVENGWE FFKRLQTADG HWGCNDDGPL FVTSGMVIAR
     YIVGIPIDSH MKQEMCRYLL NVVNEDGGWG LFIQSPSTVF GTVMNYCMLR ILGLGPEHPA
     MARARNTLHR LGSASATPTW GKFWLCVLGV YEWEGMIPLP PEPLLVPASL PFNPGKWWVH
     TRNVYISMSY LYGHRFSMPP NKLVQALRDE LYDIPYEQIN WPAQRTNVSA ADRLTDPTWI
     QRSFTSALTT YETFKIPFLR RRALNEALFQ IETETRNTHY LCIAPVSFAS NMLALYHAHG
     RDSHWIRGMR DRFIDPMWLC REGLAASGTN GTSLWDTALT VQATIDAGLA ARPENQAILR
     KALEFIDNSQ IREDPLGVHH VYRQPTRGAW PFSTRDQSYA VSDTTAEAVK VVVLLQQIEG
     FPSRISDERL QQAIDLILGM ENAGGGFSAY EPVRGPKFLE LLNITELYEN VMTDNLYPEC
     TSSVIMSLTT FAREYPTYRA RDIQACVSRS VDYLLRSQYP NGGWFASWGV CFTYATMFAL
     QGLACMGRNE SNCAACQRAC SFLLQHQNPD GGWGESLDTV RFKQYLPHPD GSQVTNTAYA
     VIGLLAARCG NHEAIRRGVA YLMKEQQDTG EWLPGALEGV FAPPGGMRYP NYKFHFTLMA
     LGRYAAVHGD ECLAAQLV