PDS_CAPAN
ID PDS_CAPAN Reviewed; 582 AA.
AC P80093;
DT 01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-1992, sequence version 1.
DT 03-AUG-2022, entry version 102.
DE RecName: Full=15-cis-phytoene desaturase, chloroplastic/chromoplastic {ECO:0000305};
DE EC=1.3.5.5 {ECO:0000269|PubMed:1396714, ECO:0000269|PubMed:15503129};
DE AltName: Full=Phytoene dehydrogenase {ECO:0000305};
DE AltName: Full=Phytoene desaturase {ECO:0000305};
DE Flags: Precursor;
GN Name=PDS;
OS Capsicum annuum (Capsicum pepper).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Solanoideae; Capsiceae; Capsicum.
OX NCBI_TaxID=4072;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, COFACTOR,
RP SUBCELLULAR LOCATION, AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Lamuyo;
RX PubMed=1396714; DOI=10.1111/j.1432-1033.1992.tb17302.x;
RA Hugueney P., Roemer S., Kuntz M., Camara B.;
RT "Characterization and molecular cloning of a flavoprotein catalyzing the
RT synthesis of phytofluene and zeta-carotene in Capsicum chromoplasts.";
RL Eur. J. Biochem. 209:399-407(1992).
RN [2]
RP FUNCTION, CATALYTIC ACTIVITY, AND PATHWAY.
RX PubMed=15503129; DOI=10.1007/s00425-004-1395-2;
RA Breitenbach J., Sandmann G.;
RT "zeta-Carotene cis isomers as products and substrates in the plant poly-cis
RT carotenoid biosynthetic pathway to lycopene.";
RL Planta 220:785-793(2005).
CC -!- FUNCTION: Converts phytoene into zeta-carotene via the intermediary of
CC phytofluene by the symmetrical introduction of two double bonds at the
CC C-11 and C-11' positions of phytoene with a concomitant isomerization
CC of two neighboring double bonds at the C9 and C9' positions from trans
CC to cis. {ECO:0000269|PubMed:1396714, ECO:0000269|PubMed:15503129}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=15-cis-phytoene + 2 a plastoquinone = 9,9',15-tri-cis-zeta-
CC carotene + 2 a plastoquinol; Xref=Rhea:RHEA:30287, Rhea:RHEA-
CC COMP:9561, Rhea:RHEA-COMP:9562, ChEBI:CHEBI:17757, ChEBI:CHEBI:27787,
CC ChEBI:CHEBI:48717, ChEBI:CHEBI:62192; EC=1.3.5.5;
CC Evidence={ECO:0000269|PubMed:1396714, ECO:0000269|PubMed:15503129};
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000269|PubMed:1396714};
CC -!- ACTIVITY REGULATION: Inhibited by the herbicides metflurazon, difunone,
CC fluridone and diflufenican.
CC -!- PATHWAY: Carotenoid biosynthesis; lycopene biosynthesis.
CC -!- SUBUNIT: Homotetramer. {ECO:0000250|UniProtKB:A2XDA1}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast. Plastid, chromoplast
CC {ECO:0000269|PubMed:1396714}. Membrane {ECO:0000269|PubMed:1396714};
CC Peripheral membrane protein {ECO:0000250|UniProtKB:A2XDA1}.
CC -!- DEVELOPMENTAL STAGE: Ripening fruit. {ECO:0000269|PubMed:1396714}.
CC -!- SIMILARITY: Belongs to the carotenoid/retinoid oxidoreductase family.
CC {ECO:0000305}.
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DR EMBL; X68058; CAA48195.1; -; mRNA.
DR PIR; S29314; S29314.
DR RefSeq; NP_001311742.1; NM_001324813.1.
DR AlphaFoldDB; P80093; -.
DR SMR; P80093; -.
DR GeneID; 107861625; -.
DR KEGG; cann:107861625; -.
DR UniPathway; UPA00803; -.
DR Proteomes; UP000189700; Genome assembly.
DR GO; GO:0009507; C:chloroplast; IDA:UniProtKB.
DR GO; GO:0009509; C:chromoplast; IDA:UniProtKB.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016166; F:phytoene dehydrogenase activity; IDA:UniProtKB.
DR GO; GO:0016120; P:carotene biosynthetic process; IDA:UniProtKB.
DR GO; GO:0016117; P:carotenoid biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0009635; P:response to herbicide; IEA:UniProtKB-KW.
DR Gene3D; 3.50.50.60; -; 1.
DR InterPro; IPR002937; Amino_oxidase.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR014102; Phytoene_desaturase.
DR Pfam; PF01593; Amino_oxidase; 1.
DR SUPFAM; SSF51905; SSF51905; 1.
DR TIGRFAMs; TIGR02731; phytoene_desat; 1.
PE 1: Evidence at protein level;
KW Carotenoid biosynthesis; Chloroplast; Chromoplast; Herbicide resistance;
KW Membrane; Oxidoreductase; Plastid; Transit peptide.
FT TRANSIT 1..110
FT /note="Chloroplast and chromoplast"
FT /evidence="ECO:0000255"
FT CHAIN 111..582
FT /note="15-cis-phytoene desaturase,
FT chloroplastic/chromoplastic"
FT /id="PRO_0000006323"
FT BINDING 140..141
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000250|UniProtKB:A2XDA1"
FT BINDING 148
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000250|UniProtKB:A2XDA1"
FT BINDING 165..166
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000250|UniProtKB:A2XDA1"
FT BINDING 171
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000250|UniProtKB:A2XDA1"
FT BINDING 306
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:A2XDA1"
FT BINDING 348
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000250|UniProtKB:A2XDA1"
FT BINDING 537
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000250|UniProtKB:A2XDA1"
FT BINDING 545
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:A2XDA1"
FT BINDING 547
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000250|UniProtKB:A2XDA1"
SQ SEQUENCE 582 AA; 65062 MW; 011E6E7DCAFB3DB5 CRC64;
MPQIGLVSAV NLRVQGNSAY LWSSRSSLGT DSQDGCSQRN SLCFGGSDSM SHRLKIRNPH
SITRRLAKDF RPLKVVCIDY PRPELDNTVN YLEAAFLSSS FRSSPRPTKP LEIVIAGAGL
GGLSTAKYLA DAGHKPILLE ARDVLGGKVA AWKDDDGDWY ETGLHIFFGA YPNMQNLFGE
LGINDRLQWK EHSMIFAMPN KPGEFSRFDF PEALPAPLNG ILAILKNNEM LTWPEKVKFA
IGLLPAMLGG QSYVEAQDGI SVKDWMRKQG VPDRVTDEVF IAMSKALNFI NPDELSMQCI
LIALNRFLQE KHGSKMAFLD GNPPERLCMP IVEHIESKGG QVRLNSRIKK IELNEDGSVK
CFILNDGSTI EGDAFVFATP VDIFKLLLPE DWKEIPYFQK LEKLVGVPVI NVHIWFDRKL
KNTSDNLLFS RSPLLSVYAD MSVTCKEYYD PNKSMLELVF APAEEWVSRS DSEIIDATMK
ELAKLFPDEI SADQSKAKIL KYHVVKTPRS VYKTVPGCEP CRLLQRSPVE GFYLAGDYTK
QKYLASMEGA VLSGKLCAQA IVQDYELLVG RSQRKLAETS VV
