PDTAS_MYCTU
ID PDTAS_MYCTU Reviewed; 501 AA.
AC P9WGL5; L0TDI0; O05846; Q7D5W7;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 16-APR-2014, sequence version 1.
DT 25-MAY-2022, entry version 40.
DE RecName: Full=Probable sensor histidine kinase PdtaS;
DE EC=2.7.13.3;
GN Name=pdtaS; OrderedLocusNames=Rv3220c;
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP FUNCTION, AND AUTOPHOSPHORYLATION.
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=16026786; DOI=10.1016/j.febslet.2005.06.043;
RA Morth J.P., Gosmann S., Nowak E., Tucker P.A.;
RT "A novel two-component system found in Mycobacterium tuberculosis.";
RL FEBS Lett. 579:4145-4148(2005).
RN [3]
RP IDENTIFICATION AS A DRUG TARGET [LARGE SCALE ANALYSIS].
RX PubMed=19099550; DOI=10.1186/1752-0509-2-109;
RA Raman K., Yeturu K., Chandra N.;
RT "targetTB: a target identification pipeline for Mycobacterium tuberculosis
RT through an interactome, reactome and genome-scale structural analysis.";
RL BMC Syst. Biol. 2:109-109(2008).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT mass spectrometry.";
RL Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC -!- FUNCTION: Member of the two-component regulatory system PdtaR/PdtaS.
CC Autophosphorylates, probably on a histidine residue, and transfers its
CC phosphate group to PdtaR. {ECO:0000269|PubMed:16026786}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + protein L-histidine = ADP + protein N-phospho-L-
CC histidine.; EC=2.7.13.3;
CC -!- PTM: Autophosphorylated.
CC -!- MISCELLANEOUS: Was identified as a high-confidence drug target.
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DR EMBL; AL123456; CCP46036.1; -; Genomic_DNA.
DR PIR; E70596; E70596.
DR RefSeq; NP_217736.1; NC_000962.3.
DR RefSeq; WP_003416886.1; NZ_NVQJ01000003.1.
DR PDB; 2YKF; X-ray; 2.00 A; A=1-303.
DR PDB; 2YKH; X-ray; 2.78 A; A/B=1-303.
DR PDBsum; 2YKF; -.
DR PDBsum; 2YKH; -.
DR AlphaFoldDB; P9WGL5; -.
DR SMR; P9WGL5; -.
DR STRING; 83332.Rv3220c; -.
DR PaxDb; P9WGL5; -.
DR DNASU; 888801; -.
DR GeneID; 45427213; -.
DR GeneID; 888801; -.
DR KEGG; mtu:Rv3220c; -.
DR TubercuList; Rv3220c; -.
DR eggNOG; COG3920; Bacteria.
DR OMA; GSIAIVH; -.
DR PhylomeDB; P9WGL5; -.
DR PHI-base; PHI:3621; -.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0005524; F:ATP binding; IDA:MTBBASE.
DR GO; GO:0000155; F:phosphorelay sensor kinase activity; IDA:MTBBASE.
DR GO; GO:0004672; F:protein kinase activity; IDA:MTBBASE.
DR GO; GO:0000160; P:phosphorelay signal transduction system; IDA:MTBBASE.
DR GO; GO:0046777; P:protein autophosphorylation; IDA:MTBBASE.
DR Gene3D; 3.30.450.280; -; 1.
DR Gene3D; 3.30.565.10; -; 1.
DR InterPro; IPR038424; H_kinase_PdtaS_GAF_sf.
DR InterPro; IPR003594; HATPase_C.
DR InterPro; IPR036890; HATPase_C_sf.
DR InterPro; IPR005467; His_kinase_dom.
DR InterPro; IPR035965; PAS-like_dom_sf.
DR InterPro; IPR022066; PdtaS_GAF.
DR InterPro; IPR011495; Sig_transdc_His_kin_sub2_dim/P.
DR Pfam; PF12282; H_kinase_N; 1.
DR Pfam; PF02518; HATPase_c; 1.
DR Pfam; PF07568; HisKA_2; 1.
DR SMART; SM00387; HATPase_c; 1.
DR SUPFAM; SSF55785; SSF55785; 1.
DR SUPFAM; SSF55874; SSF55874; 1.
DR PROSITE; PS50109; HIS_KIN; 1.
PE 1: Evidence at protein level;
KW 3D-structure; ATP-binding; Kinase; Nucleotide-binding; Phosphoprotein;
KW Reference proteome; Transferase; Two-component regulatory system.
FT CHAIN 1..501
FT /note="Probable sensor histidine kinase PdtaS"
FT /id="PRO_0000386596"
FT DOMAIN 300..495
FT /note="Histidine kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
FT MOD_RES 303
FT /note="Phosphohistidine; by autocatalysis"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
FT HELIX 4..11
FT /evidence="ECO:0007829|PDB:2YKF"
FT HELIX 16..36
FT /evidence="ECO:0007829|PDB:2YKF"
FT STRAND 38..45
FT /evidence="ECO:0007829|PDB:2YKF"
FT STRAND 51..57
FT /evidence="ECO:0007829|PDB:2YKF"
FT STRAND 60..62
FT /evidence="ECO:0007829|PDB:2YKF"
FT HELIX 77..79
FT /evidence="ECO:0007829|PDB:2YKF"
FT HELIX 81..88
FT /evidence="ECO:0007829|PDB:2YKF"
FT STRAND 112..118
FT /evidence="ECO:0007829|PDB:2YKF"
FT STRAND 121..129
FT /evidence="ECO:0007829|PDB:2YKF"
FT HELIX 133..135
FT /evidence="ECO:0007829|PDB:2YKF"
FT HELIX 141..158
FT /evidence="ECO:0007829|PDB:2YKF"
FT STRAND 165..168
FT /evidence="ECO:0007829|PDB:2YKH"
FT HELIX 177..179
FT /evidence="ECO:0007829|PDB:2YKF"
FT STRAND 181..184
FT /evidence="ECO:0007829|PDB:2YKF"
FT STRAND 188..193
FT /evidence="ECO:0007829|PDB:2YKF"
FT HELIX 195..203
FT /evidence="ECO:0007829|PDB:2YKF"
FT HELIX 215..219
FT /evidence="ECO:0007829|PDB:2YKF"
FT HELIX 220..222
FT /evidence="ECO:0007829|PDB:2YKF"
FT STRAND 223..225
FT /evidence="ECO:0007829|PDB:2YKF"
FT HELIX 226..240
FT /evidence="ECO:0007829|PDB:2YKF"
FT STRAND 248..253
FT /evidence="ECO:0007829|PDB:2YKF"
FT STRAND 256..267
FT /evidence="ECO:0007829|PDB:2YKF"
FT STRAND 270..280
FT /evidence="ECO:0007829|PDB:2YKF"
FT HELIX 282..285
FT /evidence="ECO:0007829|PDB:2YKF"
SQ SEQUENCE 501 AA; 54012 MW; DF099B268388AD00 CRC64;
MSTLGDLLAE HTVLPGSAVD HLHAVVGEWQ LLADLSFADY LMWVRRDDGV LVCVAQCRPN
TGPTVVHTDA VGTVVAANSM PLVAATFSGG VPGREGAVGQ QNSCQHDGHS VEVSPVRFGD
QVVAVLTRHQ PELAARRRSG HLETAYRLCA TDLLRMLAEG TFPDAGDVAM SRSSPRAGDG
FIRLDVDGVV SYASPNALSA YHRMGLTTEL EGVNLIDATR PLISDPFEAH EVDEHVQDLL
AGDGKGMRME VDAGGATVLL RTLPLVVAGR NVGAAILIRD VTEVKRRDRA LISKDATIRE
IHHRVKNNLQ TVAALLRLQA RRTSNAEGRE ALIESVRRVS SIALVHDALS MSVDEQVNLD
EVIDRILPIM NDVASVDRPI RINRVGDLGV LDSDRATALI MVITELVQNA IEHAFDPAAA
EGSVTIRAER SARWLDVVVH DDGLGLPQGF SLEKSDSLGL QIVRTLVSAE LDGSLGMRDA
RERGTDVVLR VPVGRRGRLM L
