PDUE_SALTY
ID PDUE_SALTY Reviewed; 173 AA.
AC O31042; Q7BV83;
DT 09-FEB-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 97.
DE RecName: Full=Propanediol dehydratase small subunit;
DE EC=4.2.1.28 {ECO:0000269|PubMed:25713376};
DE AltName: Full=Diol dehydratase small subunit;
DE Short=DDH small subunit;
DE AltName: Full=Propanediol utilization protein PduE;
GN Name=pduE {ECO:0000303|PubMed:9352910}; OrderedLocusNames=STM2042;
OS Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=99287;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, AND
RP SUBUNIT.
RC STRAIN=LT2;
RX PubMed=9352910; DOI=10.1128/jb.179.21.6633-6639.1997;
RA Bobik T.A., Xu Y., Jeter R.M., Otto K.E., Roth J.R.;
RT "Propanediol utilization genes (pdu) of Salmonella typhimurium: three genes
RT for the propanediol dehydratase.";
RL J. Bacteriol. 179:6633-6639(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], PATHWAY, SUBCELLULAR LOCATION, AND
RP INDUCTION.
RC STRAIN=LT2;
RX PubMed=10498708; DOI=10.1128/jb.181.19.5967-5975.1999;
RA Bobik T.A., Havemann G.D., Busch R.J., Williams D.S., Aldrich H.C.;
RT "The propanediol utilization (pdu) operon of Salmonella enterica serovar
RT typhimurium LT2 includes genes necessary for formation of polyhedral
RT organelles involved in coenzyme B(12)-dependent 1, 2-propanediol
RT degradation.";
RL J. Bacteriol. 181:5967-5975(1999).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=11677609; DOI=10.1038/35101614;
RA McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA Wilson R.K.;
RT "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL Nature 413:852-856(2001).
RN [4]
RP PROTEIN SEQUENCE OF 1-7, AND SUBCELLULAR LOCATION.
RC STRAIN=LT2;
RX PubMed=12923081; DOI=10.1128/jb.185.17.5086-5095.2003;
RA Havemann G.D., Bobik T.A.;
RT "Protein content of polyhedral organelles involved in coenzyme B12-
RT dependent degradation of 1,2-propanediol in Salmonella enterica serovar
RT Typhimurium LT2.";
RL J. Bacteriol. 185:5086-5095(2003).
RN [5]
RP COFACTOR.
RX PubMed=2166132; DOI=10.1099/00221287-136-5-887;
RA Jeter R.M.;
RT "Cobalamin-dependent 1,2-propanediol utilization by Salmonella
RT typhimurium.";
RL J. Gen. Microbiol. 136:887-896(1990).
RN [6]
RP INDUCTION.
RX PubMed=1312999; DOI=10.1128/jb.174.7.2253-2266.1992;
RA Bobik T.A., Ailion M., Roth J.R.;
RT "A single regulatory gene integrates control of vitamin B12 synthesis and
RT propanediol degradation.";
RL J. Bacteriol. 174:2253-2266(1992).
RN [7]
RP SUBCELLULAR LOCATION.
RC STRAIN=LT2;
RX PubMed=11844753; DOI=10.1128/jb.184.5.1253-1261.2002;
RA Havemann G.D., Sampson E.M., Bobik T.A.;
RT "PduA is a shell protein of polyhedral organelles involved in coenzyme
RT B(12)-dependent degradation of 1,2-propanediol in Salmonella enterica
RT serovar typhimurium LT2.";
RL J. Bacteriol. 184:1253-1261(2002).
RN [8]
RP DISRUPTION PHENOTYPE.
RC STRAIN=LT2;
RX PubMed=16585748; DOI=10.1128/jb.188.8.2865-2874.2006;
RA Penrod J.T., Roth J.R.;
RT "Conserving a volatile metabolite: a role for carboxysome-like organelles
RT in Salmonella enterica.";
RL J. Bacteriol. 188:2865-2874(2006).
RN [9]
RP SUBCELLULAR LOCATION, AND MUTAGENESIS OF 2-ASN--SER-35.
RC STRAIN=LT2;
RX PubMed=21821773; DOI=10.1128/jb.05661-11;
RA Fan C., Bobik T.A.;
RT "The N-terminal region of the medium subunit (PduD) packages
RT adenosylcobalamin-dependent diol dehydratase (PduCDE) into the Pdu
RT microcompartment.";
RL J. Bacteriol. 193:5623-5628(2011).
RN [10]
RP ACTIVITY REGULATION.
RC STRAIN=LT2;
RX PubMed=25713376; DOI=10.1073/pnas.1423672112;
RA Chowdhury C., Chun S., Pang A., Sawaya M.R., Sinha S., Yeates T.O.,
RA Bobik T.A.;
RT "Selective molecular transport through the protein shell of a bacterial
RT microcompartment organelle.";
RL Proc. Natl. Acad. Sci. U.S.A. 112:2990-2995(2015).
RN [11]
RP SYSTEM-MODELING, AND FUNCTION.
RC STRAIN=LT2;
RX PubMed=28475631; DOI=10.1371/journal.pcbi.1005525;
RA Jakobson C.M., Tullman-Ercek D., Slininger M.F., Mangan N.M.;
RT "A systems-level model reveals that 1,2-Propanediol utilization
RT microcompartments enhance pathway flux through intermediate
RT sequestration.";
RL PLoS Comput. Biol. 13:e1005525-e1005525(2017).
CC -!- FUNCTION: Part of the PduCDE complex that catalyzes the dehydration of
CC 1,2-propanediol (1,2-PD) to propionaldehyde. Required for S.typhimurium
CC growth on 1,2-PD as the sole carbon and energy source. Localized in the
CC bacterial microcompartment (BMC) dedicated to 1,2-PD degradation.
CC {ECO:0000305|PubMed:9352910}.
CC -!- FUNCTION: The 1,2-PD-specific bacterial microcompartment (BMC)
CC concentrates low levels of 1,2-PD catabolic enzymes, concentrates
CC volatile reaction intermediates thus enhancing pathway flux and keeps
CC the level of toxic, mutagenic propionaldehyde low.
CC {ECO:0000269|PubMed:25713376, ECO:0000305|PubMed:28475631}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=propane-1,2-diol = H2O + propanal; Xref=Rhea:RHEA:14569,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:16997, ChEBI:CHEBI:17153; EC=4.2.1.28;
CC Evidence={ECO:0000269|PubMed:9352910};
CC -!- COFACTOR:
CC Name=adenosylcob(III)alamin; Xref=ChEBI:CHEBI:18408;
CC Evidence={ECO:0000269|PubMed:2166132};
CC -!- ACTIVITY REGULATION: Inhibited by glycerol.
CC {ECO:0000269|PubMed:25713376}.
CC -!- PATHWAY: Polyol metabolism; 1,2-propanediol degradation.
CC {ECO:0000305|PubMed:10498708}.
CC -!- SUBUNIT: The propanediol dehydratase enzyme is a heterotrimeric complex
CC composed of a large (PduC), a medium (PduD) and a small (PduE) subunit.
CC {ECO:0000269|PubMed:9352910}.
CC -!- SUBCELLULAR LOCATION: Bacterial microcompartment
CC {ECO:0000269|PubMed:10498708, ECO:0000269|PubMed:11844753,
CC ECO:0000269|PubMed:12923081, ECO:0000269|PubMed:21821773}.
CC Note=Probably located inside the BMC shell.
CC {ECO:0000269|PubMed:10498708}.
CC -!- INDUCTION: BMC production is induced by growth on 1,2-PD vitamin B12
CC medium (PubMed:10498708). By either propanediol or glycerol.
CC {ECO:0000269|PubMed:10498708, ECO:0000269|PubMed:1312999}.
CC -!- DISRUPTION PHENOTYPE: A triple pduC-pduD-pduE deletion releases no
CC acetaldehyde when grown on propanediol. {ECO:0000269|PubMed:16585748}.
CC -!- MISCELLANEOUS: Bacterial microcompartments (BMC) 100-200 nm in cross
CC section are formed during aerobic growth on minimal 1,2-PD-B12 or
CC anaerobic growth on 1,2-PD-tetrathionate medium, but not during aerobic
CC growth on glucose, anerobic growth on glucose or pyruvate-tetrathionate
CC (PubMed:10498708). BMCs can constitute up to 10% of total cell protein
CC (PubMed:12923081). {ECO:0000269|PubMed:10498708,
CC ECO:0000269|PubMed:12923081}.
CC -!- SIMILARITY: Belongs to the diol/glycerol dehydratase small subunit
CC family. {ECO:0000305}.
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DR EMBL; AF026270; AAB84104.1; -; Genomic_DNA.
DR EMBL; AE006468; AAL20946.1; -; Genomic_DNA.
DR RefSeq; NP_460987.1; NC_003197.2.
DR RefSeq; WP_001090597.1; NC_003197.2.
DR AlphaFoldDB; O31042; -.
DR SMR; O31042; -.
DR STRING; 99287.STM2042; -.
DR PaxDb; O31042; -.
DR EnsemblBacteria; AAL20946; AAL20946; STM2042.
DR GeneID; 1253563; -.
DR KEGG; stm:STM2042; -.
DR PATRIC; fig|99287.12.peg.2164; -.
DR HOGENOM; CLU_120853_0_0_6; -.
DR OMA; CAAWFEE; -.
DR PhylomeDB; O31042; -.
DR BioCyc; MetaCyc:STM2042-MON; -.
DR BioCyc; SENT99287:STM2042-MON; -.
DR UniPathway; UPA00621; -.
DR Proteomes; UP000001014; Chromosome.
DR GO; GO:0031472; C:propanediol degradation polyhedral organelle; IDA:UniProtKB.
DR GO; GO:0031419; F:cobalamin binding; IEA:UniProtKB-KW.
DR GO; GO:0050215; F:propanediol dehydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0051144; P:propanediol catabolic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 1.10.1510.20; -; 1.
DR InterPro; IPR003207; Ppandiol/glycerol_DeHydtase_su.
DR InterPro; IPR036091; Prodiol/glycerol_DeHase__sf_su.
DR Pfam; PF02287; Dehydratase_SU; 1.
DR PIRSF; PIRSF018505; Prpndl_dhdrts_sm; 1.
DR SUPFAM; SSF47148; SSF47148; 1.
PE 1: Evidence at protein level;
KW Bacterial microcompartment; Cobalamin; Cobalt; Direct protein sequencing;
KW Lyase; Reference proteome.
FT CHAIN 1..173
FT /note="Propanediol dehydratase small subunit"
FT /id="PRO_0000391460"
FT MUTAGEN 2..35
FT /note="Missing: Enzyme associates with BMCs, 25% decrease
FT in enzyme activity."
FT /evidence="ECO:0000269|PubMed:21821773"
SQ SEQUENCE 173 AA; 19144 MW; 4E0012588EFB3916 CRC64;
MNTDAIESMV RDVLSRMNSL QGDAPAAAPA AGGTSRSAKV SDYPLANKHP EWVKTATNKT
LDDFTLENVL SNKVTAQDMR ITPETLRLQA SIAKDAGRDR LAMNFERAAE LTAVPDDRIL
EIYNALRPYR STKEELLAIA DDLENRYQAK ICAAFVREAA GLYVERKKLK GDD
