PDV2_ARATH
ID PDV2_ARATH Reviewed; 307 AA.
AC Q9XII1; Q93ZL0;
DT 05-APR-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 25-MAY-2022, entry version 109.
DE RecName: Full=Plastid division protein PDV2 {ECO:0000303|PubMed:16998069};
DE AltName: Full=Protein PLASTID DIVISION2 {ECO:0000303|PubMed:16998069};
GN Name=PDV2 {ECO:0000303|PubMed:16998069};
GN Synonyms=PD116 {ECO:0000303|PubMed:27988788};
GN OrderedLocusNames=At2g16070 {ECO:0000312|Araport:AT2G16070};
GN ORFNames=F7H1.9 {ECO:0000312|EMBL:AAD26950.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1), FUNCTION, AND
RP DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia;
RX PubMed=16998069; DOI=10.1105/tpc.106.045484;
RA Miyagishima S.-Y., Froehlich J.E., Osteryoung K.W.;
RT "PDV1 and PDV2 mediate recruitment of the dynamin-related protein ARC5 to
RT the plastid division site.";
RL Plant Cell 18:2517-2530(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION, INTERACTION WITH ARC6, SUBCELLULAR LOCATION, DISRUPTION
RP PHENOTYPE, AND MUTAGENESIS OF GLY-307.
RC STRAIN=cv. Columbia;
RX PubMed=18812496; DOI=10.1105/tpc.108.061440;
RA Glynn J.M., Froehlich J.E., Osteryoung K.W.;
RT "Arabidopsis ARC6 coordinates the division machineries of the inner and
RT outer chloroplast membranes through interaction with PDV2 in the
RT intermembrane space.";
RL Plant Cell 20:2460-2470(2008).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-50, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=cv. Columbia;
RX PubMed=19245862; DOI=10.1016/j.jprot.2009.02.004;
RA Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E.,
RA Rathjen J.P., Peck S.C.;
RT "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis
RT thaliana.";
RL J. Proteomics 72:439-451(2009).
RN [8]
RP SUBCELLULAR LOCATION.
RX PubMed=19453460; DOI=10.1111/j.1365-313x.2009.03905.x;
RA Glynn J.M., Yang Y., Vitha S., Schmitz A.J., Hemmes M., Miyagishima S.-Y.,
RA Osteryoung K.W.;
RT "PARC6, a novel chloroplast division factor, influences FtsZ assembly and
RT is required for recruitment of PDV1 during chloroplast division in
RT Arabidopsis.";
RL Plant J. 59:700-711(2009).
RN [9]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
RN [10]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22223895; DOI=10.1074/mcp.m111.015131;
RA Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T.,
RA Giglione C.;
RT "Comparative large-scale characterisation of plant vs. mammal proteins
RT reveals similar and idiosyncratic N-alpha acetylation features.";
RL Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012).
RN [11]
RP INDUCTION BY GIBBERELLIC ACID.
RX PubMed=23020316; DOI=10.1111/j.1365-313x.2012.05118.x;
RA Jiang X., Li H., Wang T., Peng C., Wang H., Wu H., Wang X.;
RT "Gibberellin indirectly promotes chloroplast biogenesis as a means to
RT maintain the chloroplast population of expanded cells.";
RL Plant J. 72:768-780(2012).
RN [12]
RP FUNCTION.
RC STRAIN=cv. Columbia;
RX PubMed=25736058; DOI=10.1105/tpc.115.136234;
RA Okazaki K., Miyagishima S.-Y., Wada H.;
RT "Phosphatidylinositol 4-phosphate negatively regulates chloroplast division
RT in Arabidopsis.";
RL Plant Cell 27:663-674(2015).
RN [13]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RC STRAIN=cv. Columbia, and cv. Landsberg erecta;
RX PubMed=27988788; DOI=10.1007/s00299-016-2096-6;
RA Chang N., Sun Q., Li Y., Mu Y., Hu J., Feng Y., Liu X., Gao H.;
RT "PDV2 has a dosage effect on chloroplast division in Arabidopsis.";
RL Plant Cell Rep. 36:471-480(2017).
RN [14]
RP FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, AND INTERACTION WITH
RP ARC5/DRP5B.
RC STRAIN=cv. Columbia, and cv. Landsberg erecta;
RX PubMed=32005784; DOI=10.1104/pp.19.01490;
RA Sun B., Zhang Q.-Y., Yuan H., Gao W., Han B., Zhang M.;
RT "PDV1 and PDV2 differentially affect remodeling and assembly of the
RT chloroplast DRP5B ring.";
RL Plant Physiol. 182:1966-1978(2020).
RN [15]
RP X-RAY CRYSTALLOGRAPHY (2.87 ANGSTROMS) OF 284-307 IN COMPLEX WITH ARC6,
RP FUNCTION, MUTAGENESIS OF ARG-288, DISRUPTION PHENOTYPE, AND INTERACTION
RP WITH ARC6.
RX PubMed=28248291; DOI=10.1038/nplants.2017.11;
RA Wang W., Li J., Sun Q., Yu X., Zhang W., Jia N., An C., Li Y., Dong Y.,
RA Han F., Chang N., Liu X., Zhu Z., Yu Y., Fan S., Yang M., Luo S.-Z.,
RA Gao H., Feng Y.;
RT "Structural insights into the coordination of plastid division by the ARC6-
RT PDV2 complex.";
RL Nat. Plants 3:17011-17011(2017).
CC -!- FUNCTION: Component of the plastid division machinery consisting in a
CC binary fission accomplished by the simultaneous constriction of the
CC FtsZ ring on the stromal side of the inner envelope membrane, and the
CC ARC5/DRP5B ring on the cytosolic side of the outer envelope membrane
CC (PubMed:28248291). Positive factor of chloroplast division required,
CC with a dosage effect, to mediate the recruitment and dimerization of
CC ARC5/DRP5B at the midplastid constriction site in the cytoplasm at
CC plastid outer envelope membranes (OEMs) (PubMed:28248291,
CC PubMed:27988788, PubMed:32005784). Prevents ARC5/DRP5B GTPase acrivity
CC (PubMed:27988788). Relays plastid division site position between stroma
CC and outer surface via interactions with the cytoplasmic ARC5/DRP5B and
CC the inner membrane ARC6 that recruits stromal FtsZ ring
CC (PubMed:28248291). Binding to phosphatidylinositol 4-phosphate (PI4P)
CC modulates negatively chloroplast division (PubMed:25736058).
CC {ECO:0000269|PubMed:16998069, ECO:0000269|PubMed:18812496,
CC ECO:0000269|PubMed:25736058, ECO:0000269|PubMed:27988788,
CC ECO:0000269|PubMed:28248291, ECO:0000269|PubMed:32005784}.
CC -!- SUBUNIT: Interacts (via C-terminus) with ARC6 (via C-terminus) in the
CC chloroplast intermembrane space; this interaction induces ARC6
CC homodimerization and leads to the formation of an heterotetramer
CC containing two ARC6 and two PDV2 subunits (PubMed:18812496,
CC PubMed:28248291). Interacts with ARC5/DRP5B (PubMed:32005784).
CC {ECO:0000269|PubMed:18812496, ECO:0000269|PubMed:28248291,
CC ECO:0000269|PubMed:32005784}.
CC -!- INTERACTION:
CC Q9XII1; Q9FIG9: ARC6; NbExp=3; IntAct=EBI-2000823, EBI-2000800;
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast outer membrane
CC {ECO:0000269|PubMed:18812496, ECO:0000269|PubMed:19453460,
CC ECO:0000269|PubMed:32005784}; Single-pass membrane protein
CC {ECO:0000269|PubMed:18812496, ECO:0000269|PubMed:19453460}.
CC Note=Plastid equatorial positioning in a discontinuous ring mediated by
CC ARC6 (PubMed:18812496). Colocalizes with ARC5/DRP5B at the chloroplast
CC division site (PubMed:32005784). {ECO:0000269|PubMed:18812496,
CC ECO:0000269|PubMed:32005784}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9XII1-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9XII1-2; Sequence=VSP_040897;
CC -!- TISSUE SPECIFICITY: Mostly expressed in young leaves.
CC {ECO:0000269|PubMed:27988788}.
CC -!- DEVELOPMENTAL STAGE: Highest levels in young developing leaves with a
CC sharp expression decrease in fast-growing and mature leaves (at protein
CC level). {ECO:0000269|PubMed:27988788}.
CC -!- INDUCTION: Slightly induced by gibberellic acid (GA).
CC {ECO:0000269|PubMed:23020316}.
CC -!- DISRUPTION PHENOTYPE: Reduced number of constricted and large
CC chloroplasts (PubMed:16998069, PubMed:18812496). Attenuated PDV2-
CC induced ARC6 dimerization in the chloroplast intermembrane space
CC leading to an abnormal morphology of ARC6 rings thus compromizing
CC plastidial division (PubMed:28248291). Altered localization of
CC ARC5/DRP5B in cytosol only rather than at the constriction sites of
CC enlarged chloroplasts (PubMed:32005784). In pd116, reduced number of
CC dumbbell-shaped and large chloroplasts in mesophyll cells, with
CC sometimes unique giant chloroplasts (PubMed:27988788).
CC {ECO:0000269|PubMed:16998069, ECO:0000269|PubMed:18812496,
CC ECO:0000269|PubMed:27988788, ECO:0000269|PubMed:28248291,
CC ECO:0000269|PubMed:32005784}.
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DR EMBL; AB252217; BAF36495.1; -; Genomic_DNA.
DR EMBL; AB252219; BAF36497.1; -; mRNA.
DR EMBL; AC007134; AAD26950.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC06464.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC06465.1; -; Genomic_DNA.
DR EMBL; CP002685; ANM62193.1; -; Genomic_DNA.
DR EMBL; AY056812; AAL10503.1; -; mRNA.
DR EMBL; AY080829; AAL87305.1; -; mRNA.
DR EMBL; AY113976; AAM45024.1; -; mRNA.
DR EMBL; AY088807; AAM67117.1; -; mRNA.
DR PIR; D84536; D84536.
DR RefSeq; NP_001324370.1; NM_001335463.1. [Q9XII1-2]
DR RefSeq; NP_028242.1; NM_127166.4. [Q9XII1-1]
DR RefSeq; NP_849959.1; NM_179628.4. [Q9XII1-2]
DR PDB; 5GTB; X-ray; 2.87 A; B=284-307.
DR PDBsum; 5GTB; -.
DR AlphaFoldDB; Q9XII1; -.
DR SMR; Q9XII1; -.
DR BioGRID; 1463; 6.
DR IntAct; Q9XII1; 3.
DR STRING; 3702.AT2G16070.2; -.
DR iPTMnet; Q9XII1; -.
DR PaxDb; Q9XII1; -.
DR PRIDE; Q9XII1; -.
DR ProteomicsDB; 251401; -. [Q9XII1-1]
DR EnsemblPlants; AT2G16070.1; AT2G16070.1; AT2G16070. [Q9XII1-2]
DR EnsemblPlants; AT2G16070.2; AT2G16070.2; AT2G16070. [Q9XII1-1]
DR EnsemblPlants; AT2G16070.3; AT2G16070.3; AT2G16070. [Q9XII1-2]
DR GeneID; 816104; -.
DR Gramene; AT2G16070.1; AT2G16070.1; AT2G16070. [Q9XII1-2]
DR Gramene; AT2G16070.2; AT2G16070.2; AT2G16070. [Q9XII1-1]
DR Gramene; AT2G16070.3; AT2G16070.3; AT2G16070. [Q9XII1-2]
DR KEGG; ath:AT2G16070; -.
DR Araport; AT2G16070; -.
DR TAIR; locus:2052996; AT2G16070.
DR eggNOG; ENOG502REYJ; Eukaryota.
DR HOGENOM; CLU_075429_0_0_1; -.
DR InParanoid; Q9XII1; -.
DR OMA; ANHERSE; -.
DR OrthoDB; 1188785at2759; -.
DR PhylomeDB; Q9XII1; -.
DR PRO; PR:Q9XII1; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9XII1; baseline and differential.
DR Genevisible; Q9XII1; AT.
DR GO; GO:0009707; C:chloroplast outer membrane; IDA:UniProtKB.
DR GO; GO:0031359; C:integral component of chloroplast outer membrane; IDA:UniProtKB.
DR GO; GO:0005739; C:mitochondrion; HDA:TAIR.
DR GO; GO:0009536; C:plastid; HDA:TAIR.
DR GO; GO:0070273; F:phosphatidylinositol-4-phosphate binding; IDA:UniProtKB.
DR GO; GO:0010020; P:chloroplast fission; IMP:UniProtKB.
DR GO; GO:0009739; P:response to gibberellin; IEP:UniProtKB.
DR InterPro; IPR038939; PDV1/PDV2.
DR PANTHER; PTHR33600; PTHR33600; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Alternative splicing; Chloroplast; Coiled coil;
KW Membrane; Phosphoprotein; Plastid; Plastid outer membrane;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..307
FT /note="Plastid division protein PDV2"
FT /id="PRO_0000406944"
FT TOPO_DOM 1..213
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 214..234
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 235..307
FT /note="Chloroplast intermembrane"
FT /evidence="ECO:0000255"
FT REGION 28..66
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 174..206
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 235..307
FT /note="ARC6 binding"
FT /evidence="ECO:0000269|PubMed:18812496,
FT ECO:0000269|PubMed:28248291, ECO:0007744|PDB:5GTB"
FT COILED 76..103
FT /evidence="ECO:0000255"
FT COMPBIAS 41..62
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0007744|PubMed:22223895"
FT MOD_RES 50
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19245862"
FT VAR_SEQ 1..93
FT /note="MEDEEGIGLILARATELRLKISDCIDNSSTTVSDNGDGNEDLSPGEGRKSEI
FT IGNQDKDFDSISSEDVDEAEAERLLRIRDALEALESQLASL -> MWGWFLVCGE (in
FT isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172"
FT /id="VSP_040897"
FT MUTAGEN 288
FT /note="R->K: In pdv2-4; impaired interaction with PDV2
FT leading to altered chloroplast division and fewer but
FT larger chloroplasts."
FT /evidence="ECO:0000269|PubMed:28248291"
FT MUTAGEN 307
FT /note="G->D: Impaired ARC6 interaction, and reduced number
FT of constricted and large chloroplasts."
FT /evidence="ECO:0000269|PubMed:18812496"
FT STRAND 289..291
FT /evidence="ECO:0007829|PDB:5GTB"
SQ SEQUENCE 307 AA; 33641 MW; F2EE488B3257303B CRC64;
MEDEEGIGLI LARATELRLK ISDCIDNSST TVSDNGDGNE DLSPGEGRKS EIIGNQDKDF
DSISSEDVDE AEAERLLRIR DALEALESQL ASLQNLRQRQ QYEKQLALSE IDYSRKMLLE
KLKEYKGKDF EVLRETTTFA GERVDYENDL LLPPYPVHPP LSLGLDNNNG YLSHLPSKKK
SDANGFGSGH VRNEAEAKSP NGGSGGSSHG VIRFLGSVAK IVLPIIGVIS LLSASGYGPE
MRKRGASLNL FGLLPHRATR GKRTPNQCPP GKVLVIEDGE ARCLVKERVE IPFDSVVAKR
DVTYGYG
