ID   A43_VACCW               Reviewed;         194 AA.
AC   P26671; Q76ZN4;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 1.
DT   02-JUN-2021, entry version 55.
DE   RecName: Full=Protein A43;
DE   Flags: Precursor;
GN   OrderedLocusNames=VACWR168; ORFNames=A43R;
OS   Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain
OS   WR)).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=10254;
OH   NCBI_TaxID=9913; Bos taurus (Bovine).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1870190; DOI=10.1128/jvi.65.9.4598-4608.1991;
RA   Blasco R., Cole N.B., Moss B.;
RT   "Sequence analysis, expression, and deletion of a vaccinia virus gene
RT   encoding a homolog of profilin, a eukaryotic actin-binding protein.";
RL   J. Virol. 65:4598-4608(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2045793; DOI=10.1099/0022-1317-72-6-1349;
RA   Smith G.L., Chan Y.S., Howard S.T.;
RT   "Nucleotide sequence of 42 kbp of vaccinia virus strain WR from near the
RT   right inverted terminal repeat.";
RL   J. Gen. Virol. 72:1349-1376(1991).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J.,
RA   Wohlhueter R.;
RT   "Sequencing of the coding region of Vaccinia-WR to an average 9-fold
RT   redundancy and an error rate of 0.16/10kb.";
RL   Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   SUBCELLULAR LOCATION, AND GLYCOSYLATION.
RX   PubMed=19900687; DOI=10.1016/j.virol.2009.10.025;
RA   Sood C.L., Moss B.;
RT   "Vaccinia virus A43R gene encodes an orthopoxvirus-specific late non-virion
RT   type-1 membrane protein that is dispensable for replication but enhances
RT   intradermal lesion formation.";
RL   Virology 396:160-168(2010).
CC   -!- SUBCELLULAR LOCATION: Host membrane {ECO:0000305}; Single-pass type I
CC       membrane protein {ECO:0000305}. Host cell surface
CC       {ECO:0000269|PubMed:19900687}.
CC   -!- INDUCTION: Expressed in the late phase of the viral replicative cycle.
CC   -!- SIMILARITY: Belongs to the orthopoxvirus A43R protein family.
CC       {ECO:0000305}.
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DR   EMBL; M72474; AAA48309.1; -; Genomic_DNA.
DR   EMBL; D11079; BAA01816.1; -; Genomic_DNA.
DR   EMBL; AY243312; AAO89447.1; -; Genomic_DNA.
DR   PIR; JQ1780; JQ1780.
DR   RefSeq; YP_233050.1; NC_006998.1.
DR   iPTMnet; P26671; -.
DR   DNASU; 3707698; -.
DR   GeneID; 3707698; -.
DR   KEGG; vg:3707698; -.
DR   Proteomes; UP000000344; Genome.
DR   GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0044228; C:host cell surface; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   InterPro; IPR009487; Orthopox_A43R.
DR   Pfam; PF06517; Orthopox_A43R; 1.
PE   1: Evidence at protein level;
KW   Glycoprotein; Host membrane; Late protein; Membrane; Reference proteome;
KW   Signal; Transmembrane; Transmembrane helix.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..194
FT                   /note="Protein A43"
FT                   /id="PRO_0000099332"
FT   TOPO_DOM        23..165
FT                   /note="Extracellular"
FT   TRANSMEM        166..186
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        187..194
FT                   /note="Cytoplasmic"
FT   CARBOHYD        65
FT                   /note="N-linked (GlcNAc...) asparagine; by host"
FT                   /evidence="ECO:0000269|PubMed:19900687"
FT   CARBOHYD        114
FT                   /note="N-linked (GlcNAc...) asparagine; by host"
FT                   /evidence="ECO:0000269|PubMed:19900687"
SQ   SEQUENCE   194 AA;  22577 MW;  639990229C06692C CRC64;
     MMMMKWIISI LTMSIMPVLA YSSSIFRFHS EDVELCYGHL YFDRIYNVVN IKYNPHIPYR
     YNFINRTLTV DELDDNVFFT HGYFLKHKYG SLNPSLIVSL SGNLKYNDIQ CSVNVSCLIK
     NLATSTSTIL TSKHKTYSLH RSTCITIIGY DSIIWYKDIN DKYNGIYDFT AICMLIASTL
     IVTIYVFKKI KMNS