ID   PE03_MYCTU              Reviewed;         468 AA.
AC   Q79G04; I6XUK4; L0T2V2;
DT   02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 88.
DE   RecName: Full=PE family protein PE3 {ECO:0000305};
GN   Name=PE3 {ECO:0000312|EMBL:CCP42885.1};
GN   OrderedLocusNames=Rv0159c {ECO:0000312|EMBL:CCP42885.1};
GN   ORFNames=LH57_00895 {ECO:0000312|EMBL:AIR12882.1};
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 27294 / TMC 102 / H37Rv;
RA   Hazbon M.H., Riojas M.A., Damon A.M., Alalade R.O., Cantwell B.J.,
RA   Monaco A., King S., Sohrabi A.;
RT   "Phylogenetic analysis of Mycobacterial species using whole genome
RT   sequences.";
RL   Submitted (SEP-2014) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   FUNCTION, SUBCELLULAR LOCATION, INDUCTION, AND BIOTECHNOLOGY.
RC   STRAIN=H37Rv;
RX   PubMed=23708146; DOI=10.1007/s00430-013-0299-9;
RA   Singh S.K., Kumari R., Singh D.K., Tiwari S., Singh P.K., Sharma S.,
RA   Srivastava K.K.;
RT   "Putative roles of a proline-glutamic acid-rich protein (PE3) in
RT   intracellular survival and as a candidate for subunit vaccine against
RT   Mycobacterium tuberculosis.";
RL   Med. Microbiol. Immunol. 202:365-377(2013).
CC   -!- FUNCTION: Plays significant roles in mycobacterial persistence during
CC       infection and modulates host immune response.
CC       {ECO:0000269|PubMed:23708146}.
CC   -!- SUBCELLULAR LOCATION: Secreted, cell wall
CC       {ECO:0000269|PubMed:23708146}.
CC   -!- INDUCTION: Expression is high during exponential phase. Induced during
CC       hypoxic conditions. Induced in the lung and in spleen tissues of mice
CC       during the early and chronic stages of infection.
CC       {ECO:0000269|PubMed:23708146}.
CC   -!- BIOTECHNOLOGY: Could be a prospective candidate for the development of
CC       subunit vaccine against tuberculosis. {ECO:0000269|PubMed:23708146}.
CC   -!- MISCELLANEOUS: The immunization of mice with recombinant PE3 protein
CC       stimulates the secretion of TNF, IL-6 and IL-2 cytokines and generates
CC       strong protective immunity against challenge with live mycobacteria.
CC       {ECO:0000269|PubMed:23708146}.
CC   -!- SIMILARITY: Belongs to the mycobacterial PE family. {ECO:0000305}.
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DR   EMBL; AL123456; CCP42885.1; -; Genomic_DNA.
DR   EMBL; CP009480; AIR12882.1; -; Genomic_DNA.
DR   RefSeq; WP_003911094.1; NZ_NVQJ01000001.1.
DR   RefSeq; YP_177697.1; NC_000962.3.
DR   AlphaFoldDB; Q79G04; -.
DR   STRING; 83332.Rv0159c; -.
DR   ESTHER; myctu-Rv0159c; PE-PPE.
DR   PaxDb; Q79G04; -.
DR   DNASU; 886826; -.
DR   GeneID; 886826; -.
DR   KEGG; mtu:Rv0159c; -.
DR   PATRIC; fig|83332.111.peg.186; -.
DR   TubercuList; Rv0159c; -.
DR   eggNOG; COG0657; Bacteria.
DR   eggNOG; COG5651; Bacteria.
DR   HOGENOM; CLU_028265_0_1_11; -.
DR   OMA; MMIGNIN; -.
DR   PhylomeDB; Q79G04; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR000084; PE-PGRS_N.
DR   InterPro; IPR013228; PE-PPE_C.
DR   Pfam; PF00934; PE; 1.
DR   Pfam; PF08237; PE-PPE; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   1: Evidence at protein level;
KW   Cell wall; Reference proteome; Secreted; Virulence.
FT   CHAIN           1..468
FT                   /note="PE family protein PE3"
FT                   /id="PRO_0000438129"
FT   DOMAIN          1..92
FT                   /note="PE"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          154..375
FT                   /note="PE-PPE"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   468 AA;  48608 MW;  BF63E94B466D9468 CRC64;
     MSYVIAAPEM LATTAADVDG IGSAIRAASA SAAGPTTGLL AAAADEVSSA AAALFSEYAR
     ECQEVLKQAA AFHGEFTRAL AAAGAAYAQA EASNTAAMSG TAGSSGALGS VGMLSGNPLT
     ALMMGGTGEP ILSDRVLAII DSAYIRPIFG PNNPVAQYTP EQWWPFIGNL SLDQSIAQGV
     TLLNNGINAE LQNGHDVVVF GYSQSAAVAT NEIRALMALP PGQAPDPSRL AFTLIGNINN
     PNGGVLERYV GLYLPFLDMS FNGATPPDSP YQTYMYTGQY DGYAHNPQYP LNILSDLNAF
     MGIRWVHNAY PFTAAEVANA VPLPTSPGYT GNTHYYMFLT QDLPLLQPIR AIPFVGTPIA
     ELIQPDLRVL VDLGYGYGYA DVPTPASLFA PINPIAVASA LATGTVQGPQ AALVSIGLLP
     QSALPNTYPY LPSANPGLMF NFGQSSVTEL SVLSGALGSV ARLIPPIA