PE05_MYCTU
ID PE05_MYCTU Reviewed; 102 AA.
AC L7N695; I6Y7F5;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 06-MAR-2013, sequence version 1.
DT 03-AUG-2022, entry version 61.
DE RecName: Full=PE family immunomodulator PE5 {ECO:0000305};
GN Name=PE5 {ECO:0000303|PubMed:23284742, ECO:0000312|EMBL:CCP43015.1};
GN OrderedLocusNames=Rv0285 {ECO:0000312|EMBL:CCP43015.1};
GN ORFNames=LH57_01560 {ECO:0000312|EMBL:AIR13008.1};
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27294 / TMC 102 / H37Rv;
RA Hazbon M.H., Riojas M.A., Damon A.M., Alalade R.O., Cantwell B.J.,
RA Monaco A., King S., Sohrabi A.;
RT "Phylogenetic analysis of Mycobacterial species using whole genome
RT sequences.";
RL Submitted (SEP-2014) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT mass spectrometry.";
RL Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
RN [4]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=23284742; DOI=10.1371/journal.pone.0051686;
RA Tiwari B.M., Kannan N., Vemu L., Raghunand T.R.;
RT "The Mycobacterium tuberculosis PE proteins Rv0285 and Rv1386 modulate
RT innate immunity and mediate bacillary survival in macrophages.";
RL PLoS ONE 7:E51686-E51686(2012).
RN [5]
RP FUNCTION IN IRON ACQUISITION, SUBCELLULAR LOCATION, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=26729876; DOI=10.1073/pnas.1523321113;
RA Tufariello J.M., Chapman J.R., Kerantzas C.A., Wong K.W., Vilcheze C.,
RA Jones C.M., Cole L.E., Tinaztepe E., Thompson V., Fenyoe D., Niederweis M.,
RA Ueberheide B., Philips J.A., Jacobs W.R. Jr.;
RT "Separable roles for Mycobacterium tuberculosis ESX-3 effectors in iron
RT acquisition and virulence.";
RL Proc. Natl. Acad. Sci. U.S.A. 113:E348-E357(2016).
CC -!- FUNCTION: Important for the siderophore-mediated iron-acquisition
CC function of ESX-3 (PubMed:26729876). May play a pivotal role in the
CC evasion of host immune response by M.tuberculosis. Mediates production
CC of IL-10 via activation of the p38 and ERK1/2 mitogen-activated protein
CC kinase (MAPK) signaling pathways (PubMed:23284742).
CC {ECO:0000269|PubMed:23284742, ECO:0000269|PubMed:26729876}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:26729876}. Cell
CC envelope {ECO:0000269|PubMed:23284742}. Cell surface
CC {ECO:0000269|PubMed:23284742}. Note=Secreted via the ESX-3 / type VII
CC secretion system (T7SS) (PubMed:26729876). Secretion is dependent on
CC EsxG and EsxH (PubMed:26729876). {ECO:0000269|PubMed:26729876}.
CC -!- DISRUPTION PHENOTYPE: PE5-PPE4 double mutant exhibits a severe iron
CC phenotype but secretes EsxG and EsxH normally. Mildly attenuated in
CC vivo. {ECO:0000269|PubMed:26729876}.
CC -!- SIMILARITY: Belongs to the mycobacterial PE family. {ECO:0000305}.
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DR EMBL; AL123456; CCP43015.1; -; Genomic_DNA.
DR EMBL; CP009480; AIR13008.1; -; Genomic_DNA.
DR RefSeq; WP_003401485.1; NZ_NVQJ01000026.1.
DR RefSeq; YP_177710.1; NC_000962.3.
DR PDB; 6UUJ; X-ray; 3.00 A; A/D/G/J=6-102.
DR PDB; 6VHR; X-ray; 3.30 A; A=6-102.
DR PDBsum; 6UUJ; -.
DR PDBsum; 6VHR; -.
DR AlphaFoldDB; L7N695; -.
DR SASBDB; L7N695; -.
DR SMR; L7N695; -.
DR STRING; 83332.Rv0285; -.
DR PaxDb; L7N695; -.
DR PRIDE; L7N695; -.
DR DNASU; 886608; -.
DR GeneID; 45424259; -.
DR GeneID; 886608; -.
DR KEGG; mtu:Rv0285; -.
DR PATRIC; fig|83332.111.peg.321; -.
DR TubercuList; Rv0285; -.
DR eggNOG; ENOG50320GU; Bacteria.
DR HOGENOM; CLU_162350_0_0_11; -.
DR OMA; QEHSAMA; -.
DR PhylomeDB; L7N695; -.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0009986; C:cell surface; IEA:UniProtKB-SubCell.
DR GO; GO:0031975; C:envelope; IEA:UniProtKB-SubCell.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR InterPro; IPR000084; PE-PGRS_N.
DR Pfam; PF00934; PE; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Reference proteome; Secreted; Virulence.
FT CHAIN 1..102
FT /note="PE family immunomodulator PE5"
FT /id="PRO_5007687871"
FT DOMAIN 3..92
FT /note="PE"
FT /evidence="ECO:0000255"
FT HELIX 11..30
FT /evidence="ECO:0007829|PDB:6UUJ"
FT HELIX 32..36
FT /evidence="ECO:0007829|PDB:6UUJ"
FT STRAND 41..44
FT /evidence="ECO:0007829|PDB:6UUJ"
FT HELIX 45..73
FT /evidence="ECO:0007829|PDB:6UUJ"
SQ SEQUENCE 102 AA; 9566 MW; 29F298118C7CAE07 CRC64;
MTLRVVPEGL AAASAAVEAL TARLAAAHAS AAPVITAVVP PAADPVSLQT AAGFSAQGVE
HAVVTAEGVE ELGRAGVGVG ESGASYLAGD AAAAATYGVV GG
