ID   PE1A_PHOEV              Reviewed;          34 AA.
AC   P0DQN1;
DT   02-DEC-2020, integrated into UniProtKB/Swiss-Prot.
DT   02-DEC-2020, sequence version 1.
DT   25-MAY-2022, entry version 6.
DE   RecName: Full=Beta/mu-theraphotoxin-Pe1a {ECO:0000303|PubMed:32511987};
DE            Short=Beta/mu-TRTX-Pe1a {ECO:0000303|PubMed:32511987};
OS   Phormingochilus everetti (Malaysian purple earth tiger tarantula).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Mygalomorphae; Theraphosidae; Phormingochilus.
OX   NCBI_TaxID=2751878;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, AND SUBCELLULAR LOCATION.
RC   TISSUE=Venom;
RX   PubMed=32511987; DOI=10.1016/j.bcp.2020.114080;
RA   Rupasinghe D.B., Herzig V., Vetter I., Dekan Z., Gilchrist J., Bosmans F.,
RA   Alewood P.F., Lewis R.J., King G.F.;
RT   "Mutational analysis of ProTx-I and the novel venom peptide Pe1b provide
RT   insight into residues responsible for selective inhibition of the analgesic
RT   drug target NaV1.7.";
RL   Biochem. Pharmacol. 181:114080-114080(2020).
CC   -!- FUNCTION: Ion channel impairing toxin that inhibits voltage-gated
CC       sodium channels. The recombinantly expressed toxin shows a weak
CC       activity against Nav1.7/SCN9A (25% inhibition at 10 uM), and shifts the
CC       voltage dependence of channel activation to more depolarized
CC       potentials. {ECO:0000269|PubMed:32511987}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:32511987}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:32511987}.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin.
CC       {ECO:0000250|UniProtKB:P83480}.
CC   -!- SIMILARITY: Belongs to the neurotoxin 10 (Hwtx-1) family. 54 (ProTx-1)
CC       subfamily. {ECO:0000305}.
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DR   AlphaFoldDB; P0DQN1; -.
DR   SMR; P0DQN1; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008200; F:ion channel inhibitor activity; IEA:InterPro.
DR   GO; GO:0017080; F:sodium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR011696; Huwentoxin-1.
DR   Pfam; PF07740; Toxin_12; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Ion channel impairing toxin;
KW   Knottin; Neurotoxin; Secreted; Toxin;
KW   Voltage-gated sodium channel impairing toxin.
FT   CHAIN           1..34
FT                   /note="Beta/mu-theraphotoxin-Pe1a"
FT                   /evidence="ECO:0000269|PubMed:32511987"
FT                   /id="PRO_0000451451"
FT   DISULFID        2..16
FT                   /evidence="ECO:0000250|UniProtKB:P83480"
FT   DISULFID        9..21
FT                   /evidence="ECO:0000250|UniProtKB:P83480"
FT   DISULFID        15..28
FT                   /evidence="ECO:0000250|UniProtKB:P83480"
SQ   SEQUENCE   34 AA;  4075 MW;  81576C766787C24B CRC64;
     ECKYLWGTCS KDEDCCAHLG CNRKHDWCGW DYTF