PE2R1_HUMAN
ID PE2R1_HUMAN Reviewed; 402 AA.
AC P34995; Q5U5U4; Q86UH3; Q86VB5; Q8NHB2;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2005, sequence version 3.
DT 03-AUG-2022, entry version 173.
DE RecName: Full=Prostaglandin E2 receptor EP1 subtype;
DE Short=PGE receptor EP1 subtype;
DE Short=PGE2 receptor EP1 subtype;
DE AltName: Full=Prostanoid EP1 receptor;
GN Name=PTGER1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND VARIANT THR-71.
RX PubMed=8253813; DOI=10.1016/s0021-9258(19)74379-8;
RA Funk C.D., Furci L., Fitzgerald G.A., Grygorczyk R., Rochette C.,
RA Bayne M.A., Abramovitz M., Adam M., Metters K.M.;
RT "Cloning and expression of a cDNA for the human prostaglandin E receptor
RT EP1 subtype.";
RL J. Biol. Chem. 268:26767-26772(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Suwa M., Sato T., Okouchi I., Arita M., Futami K., Matsumoto S.,
RA Tsutsumi S., Aburatani H., Asai K., Akiyama Y.;
RT "Genome-wide discovery and analysis of human seven transmembrane helix
RT receptor genes.";
RL Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT THR-71.
RC TISSUE=Kidney;
RA Warren C.N., Aronstam R.S., Sharma S.V.;
RT "cDNA clones of human proteins involved in signal transduction sequenced by
RT the Guthrie cDNA resource center (www.cdna.org).";
RL Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Ovary, and Pancreas;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Receptor for prostaglandin E2 (PGE2). The activity of this
CC receptor is mediated by G(q) proteins which activate a
CC phosphatidylinositol-calcium second messenger system. May play a role
CC as an important modulator of renal function. Implicated the smooth
CC muscle contractile response to PGE2 in various tissues.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Abundant in kidney. Lower level expression in lung,
CC skeletal muscle and spleen, lowest expression in testis and not
CC detected in liver brain and heart.
CC -!- PTM: Phosphorylated. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC05723.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; L22647; AAC37539.1; -; mRNA.
DR EMBL; AB065464; BAC05723.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AY275470; AAP32302.1; -; mRNA.
DR EMBL; BC039034; AAH39034.1; -; mRNA.
DR EMBL; BC051286; AAH51286.1; -; mRNA.
DR CCDS; CCDS12309.1; -.
DR PIR; A49690; A49690.
DR RefSeq; NP_000946.2; NM_000955.2.
DR AlphaFoldDB; P34995; -.
DR SMR; P34995; -.
DR BioGRID; 111703; 3.
DR STRING; 9606.ENSP00000292513; -.
DR BindingDB; P34995; -.
DR ChEMBL; CHEMBL1811; -.
DR DrugBank; DB00770; Alprostadil.
DR DrugBank; DB00905; Bimatoprost.
DR DrugBank; DB00297; Bupivacaine.
DR DrugBank; DB00429; Carboprost tromethamine.
DR DrugBank; DB00917; Dinoprostone.
DR DrugBank; DB01088; Iloprost.
DR DrugBank; DB09211; Limaprost.
DR DrugBank; DB00929; Misoprostol.
DR DrugCentral; P34995; -.
DR GuidetoPHARMACOLOGY; 340; -.
DR TCDB; 9.A.14.9.1; the g-protein-coupled receptor (gpcr) family.
DR GlyGen; P34995; 2 sites.
DR PhosphoSitePlus; P34995; -.
DR BioMuta; PTGER1; -.
DR DMDM; 83287886; -.
DR PaxDb; P34995; -.
DR PeptideAtlas; P34995; -.
DR PRIDE; P34995; -.
DR Antibodypedia; 13701; 263 antibodies from 32 providers.
DR DNASU; 5731; -.
DR Ensembl; ENST00000292513.4; ENSP00000292513.3; ENSG00000160951.4.
DR GeneID; 5731; -.
DR KEGG; hsa:5731; -.
DR MANE-Select; ENST00000292513.4; ENSP00000292513.3; NM_000955.3; NP_000946.2.
DR UCSC; uc002mys.3; human.
DR CTD; 5731; -.
DR DisGeNET; 5731; -.
DR GeneCards; PTGER1; -.
DR HGNC; HGNC:9593; PTGER1.
DR HPA; ENSG00000160951; Tissue enhanced (kidney, lymphoid tissue).
DR MIM; 176802; gene.
DR neXtProt; NX_P34995; -.
DR OpenTargets; ENSG00000160951; -.
DR PharmGKB; PA286; -.
DR VEuPathDB; HostDB:ENSG00000160951; -.
DR eggNOG; KOG3656; Eukaryota.
DR GeneTree; ENSGT01030000234559; -.
DR HOGENOM; CLU_045991_3_0_1; -.
DR InParanoid; P34995; -.
DR OMA; IYLIAKC; -.
DR OrthoDB; 972015at2759; -.
DR PhylomeDB; P34995; -.
DR TreeFam; TF324982; -.
DR PathwayCommons; P34995; -.
DR Reactome; R-HSA-391908; Prostanoid ligand receptors.
DR Reactome; R-HSA-416476; G alpha (q) signalling events.
DR SignaLink; P34995; -.
DR SIGNOR; P34995; -.
DR BioGRID-ORCS; 5731; 11 hits in 1072 CRISPR screens.
DR GeneWiki; EP1_receptor; -.
DR GenomeRNAi; 5731; -.
DR Pharos; P34995; Tclin.
DR PRO; PR:P34995; -.
DR Proteomes; UP000005640; Chromosome 19.
DR RNAct; P34995; protein.
DR Bgee; ENSG00000160951; Expressed in metanephros cortex and 96 other tissues.
DR Genevisible; P34995; HS.
DR GO; GO:0005887; C:integral component of plasma membrane; TAS:ProtInc.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0031748; F:D1 dopamine receptor binding; IEA:Ensembl.
DR GO; GO:0004957; F:prostaglandin E receptor activity; IBA:GO_Central.
DR GO; GO:0007191; P:adenylate cyclase-activating dopamine receptor signaling pathway; IEA:Ensembl.
DR GO; GO:0007189; P:adenylate cyclase-activating G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; TAS:ProtInc.
DR GO; GO:0006954; P:inflammatory response; IBA:GO_Central.
DR GO; GO:0007204; P:positive regulation of cytosolic calcium ion concentration; IBA:GO_Central.
DR GO; GO:0032496; P:response to lipopolysaccharide; IEA:Ensembl.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR008365; Prostanoid_rcpt.
DR InterPro; IPR001244; Prostglndn_DP_rcpt.
DR InterPro; IPR000708; Prostglndn_EP1_rcpt.
DR PANTHER; PTHR11866; PTHR11866; 1.
DR PANTHER; PTHR11866:SF3; PTHR11866:SF3; 1.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00428; PROSTAGLNDNR.
DR PRINTS; PR01788; PROSTANOIDR.
DR PRINTS; PR00580; PRSTNOIDEP1R.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Disulfide bond; G-protein coupled receptor; Glycoprotein;
KW Membrane; Phosphoprotein; Receptor; Reference proteome; Transducer;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..402
FT /note="Prostaglandin E2 receptor EP1 subtype"
FT /id="PRO_0000070050"
FT TOPO_DOM 1..35
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 36..62
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 63..72
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 73..96
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 97..111
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 112..133
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 134..155
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 156..177
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 178..201
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 202..227
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 228..294
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 295..321
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 322..332
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 333..354
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 355..402
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 238..266
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 8
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 25
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 110..188
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
FT VARIANT 71
FT /note="A -> T (in dbSNP:rs1057362)"
FT /evidence="ECO:0000269|PubMed:8253813, ECO:0000269|Ref.3"
FT /id="VAR_017190"
FT VARIANT 223
FT /note="T -> M (in dbSNP:rs28364042)"
FT /id="VAR_029216"
FT VARIANT 256
FT /note="H -> R (in dbSNP:rs7249305)"
FT /id="VAR_029217"
FT CONFLICT 230
FT /note="L -> H (in Ref. 1; AAC37539)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 402 AA; 41801 MW; B925359ABD86880C CRC64;
MSPCGPLNLS LAGEATTCAA PWVPNTSAVP PSGASPALPI FSMTLGAVSN LLALALLAQA
AGRLRRRRSA ATFLLFVASL LATDLAGHVI PGALVLRLYT AGRAPAGGAC HFLGGCMVFF
GLCPLLLGCG MAVERCVGVT RPLLHAARVS VARARLALAA VAAVALAVAL LPLARVGRYE
LQYPGTWCFI GLGPPGGWRQ ALLAGLFASL GLVALLAALV CNTLSGLALL RARWRRRSRR
PPPASGPDSR RRWGAHGPRS ASASSASSIA SASTFFGGSR SSGSARRARA HDVEMVGQLV
GIMVVSCICW SPMLVLVALA VGGWSSTSLQ RPLFLAVRLA SWNQILDPWV YILLRQAVLR
QLLRLLPPRA GAKGGPAGLG LTPSAWEASS LRSSRHSGLS HF
