PE2R3_BOVIN
ID PE2R3_BOVIN Reviewed; 417 AA.
AC P34979;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1994, sequence version 1.
DT 03-AUG-2022, entry version 156.
DE RecName: Full=Prostaglandin E2 receptor EP3 subtype;
DE Short=PGE receptor EP3 subtype;
DE Short=PGE2 receptor EP3 subtype;
DE AltName: Full=Prostanoid EP3 receptor;
GN Name=PTGER3;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS EP3A; EP3B; EP3C AND EP3D), FUNCTION,
RP AND SUBCELLULAR LOCATION.
RC TISSUE=Adrenal medulla;
RX PubMed=8396726; DOI=10.1038/365166a0;
RA Namba T., Sugimoto Y., Negish M., Irie A., Ushikubi F., Kakizuka A.,
RA Ito S., Ichikawa A., Narumiya S.S.;
RT "Alternative splicing of C-terminal tail of prostaglandin E receptor
RT subtype EP3 determines G-protein specificity.";
RL Nature 365:166-170(1993).
CC -!- FUNCTION: Receptor for prostaglandin E2 (PGE2) (PubMed:8396726). The
CC various isoforms have identical ligand binding properties but interact
CC with different second messenger systems: isoform EP3A couples to
CC G(i)/G(o) proteins; isoform EP3B and isoform EP3C couple to G(s), and
CC isoform EP3D couples to G(i), G(s) and G(p) (PubMed:8396726). Required
CC for normal development of fever in response to pyrinogens, including
CC IL1B, prostaglandin E2 and bacterial lipopolysaccharide (LPS). Required
CC for normal potentiation of platelet aggregation by prostaglandin E2,
CC and thus plays a role in the regulation of blood coagulation. Required
CC for increased HCO3(-) secretion in the duodenum in response to mucosal
CC acidification, and thereby contributes to the protection of the mucosa
CC against acid-induced ulceration. Not required for normal kidney
CC function, normal urine volume and osmolality (By similarity).
CC {ECO:0000250|UniProtKB:P30557, ECO:0000269|PubMed:8396726}.
CC -!- SUBUNIT: Interacts (via C-terminus) with MKLN1.
CC {ECO:0000250|UniProtKB:P34980}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:8396726};
CC Multi-pass membrane protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Comment=Additional isoforms seem to exist.;
CC Name=EP3A;
CC IsoId=P34979-1; Sequence=Displayed;
CC Name=EP3B;
CC IsoId=P34979-2; Sequence=VSP_001929, VSP_001930;
CC Name=EP3C;
CC IsoId=P34979-3; Sequence=VSP_001931, VSP_001932;
CC Name=EP3D;
CC IsoId=P34979-4; Sequence=VSP_001933, VSP_001934;
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR EMBL; D21345; BAA04811.1; -; mRNA.
DR EMBL; D21346; BAA04812.1; -; mRNA.
DR EMBL; D21347; BAA04813.1; -; mRNA.
DR EMBL; D21348; BAA04814.1; -; mRNA.
DR PIR; S36764; S36764.
DR PIR; S36765; S36765.
DR PIR; S36766; S36766.
DR PIR; S36767; S36767.
DR RefSeq; NP_851375.1; NM_181032.1. [P34979-1]
DR RefSeq; XP_005204501.1; XM_005204444.3. [P34979-3]
DR RefSeq; XP_010801729.1; XM_010803427.2.
DR AlphaFoldDB; P34979; -.
DR SMR; P34979; -.
DR STRING; 9913.ENSBTAP00000025607; -.
DR BindingDB; P34979; -.
DR PaxDb; P34979; -.
DR Ensembl; ENSBTAT00000025607; ENSBTAP00000025607; ENSBTAG00000019230. [P34979-1]
DR Ensembl; ENSBTAT00000064347; ENSBTAP00000056165; ENSBTAG00000019230. [P34979-4]
DR Ensembl; ENSBTAT00000075500; ENSBTAP00000069593; ENSBTAG00000019230. [P34979-2]
DR GeneID; 282330; -.
DR KEGG; bta:282330; -.
DR CTD; 5733; -.
DR VEuPathDB; HostDB:ENSBTAG00000019230; -.
DR eggNOG; KOG3656; Eukaryota.
DR GeneTree; ENSGT01030000234559; -.
DR HOGENOM; CLU_045991_3_0_1; -.
DR InParanoid; P34979; -.
DR OMA; SSKQWGR; -.
DR OrthoDB; 972015at2759; -.
DR TreeFam; TF324982; -.
DR Proteomes; UP000009136; Chromosome 3.
DR Bgee; ENSBTAG00000019230; Expressed in cardiac ventricle and 86 other tissues.
DR ExpressionAtlas; P34979; baseline and differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR GO; GO:0004957; F:prostaglandin E receptor activity; IBA:GO_Central.
DR GO; GO:0007189; P:adenylate cyclase-activating G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0006954; P:inflammatory response; IBA:GO_Central.
DR GO; GO:0014827; P:intestine smooth muscle contraction; IBA:GO_Central.
DR GO; GO:0060455; P:negative regulation of gastric acid secretion; IBA:GO_Central.
DR GO; GO:0007200; P:phospholipase C-activating G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0007204; P:positive regulation of cytosolic calcium ion concentration; IBA:GO_Central.
DR InterPro; IPR003304; EP3_rcpt_1.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR008365; Prostanoid_rcpt.
DR InterPro; IPR001244; Prostglndn_DP_rcpt.
DR InterPro; IPR000265; Prostglndn_EP3_rcpt.
DR PANTHER; PTHR11866; PTHR11866; 1.
DR PANTHER; PTHR11866:SF10; PTHR11866:SF10; 1.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PRINTS; PR00428; PROSTAGLNDNR.
DR PRINTS; PR01788; PROSTANOIDR.
DR PRINTS; PR00583; PRSTNOIDE31R.
DR PRINTS; PR00582; PRSTNOIDEP3R.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell membrane; G-protein coupled receptor;
KW Glycoprotein; Lipoprotein; Membrane; Palmitate; Receptor;
KW Reference proteome; Transducer; Transmembrane; Transmembrane helix.
FT CHAIN 1..417
FT /note="Prostaglandin E2 receptor EP3 subtype"
FT /id="PRO_0000070057"
FT TOPO_DOM 1..52
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 53..77
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 78..90
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 91..111
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 112..130
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 131..152
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 153..174
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 175..196
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 197..226
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 227..252
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 253..282
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 283..306
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 307..326
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 327..348
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 349..417
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 17
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 35
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 216
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 307
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 359..387
FT /note="LLKGHSYGLDTEGGTENKDKEMKENLYIS -> VANAVSSYFNDGPKVPTIS
FT LSNEITQTGA (in isoform EP3B)"
FT /evidence="ECO:0000303|PubMed:8396726"
FT /id="VSP_001929"
FT VAR_SEQ 359..385
FT /note="LLKGHSYGLDTEGGTENKDKEMKENLY -> ASPRSMWDPSSPTRDRTRVPC
FT IGSTES (in isoform EP3D)"
FT /evidence="ECO:0000303|PubMed:8396726"
FT /id="VSP_001933"
FT VAR_SEQ 359..362
FT /note="LLKG -> HVGS (in isoform EP3C)"
FT /evidence="ECO:0000303|PubMed:8396726"
FT /id="VSP_001931"
FT VAR_SEQ 363..417
FT /note="Missing (in isoform EP3C)"
FT /evidence="ECO:0000303|PubMed:8396726"
FT /id="VSP_001932"
FT VAR_SEQ 386..417
FT /note="Missing (in isoform EP3D)"
FT /evidence="ECO:0000303|PubMed:8396726"
FT /id="VSP_001934"
FT VAR_SEQ 388..417
FT /note="Missing (in isoform EP3B)"
FT /evidence="ECO:0000303|PubMed:8396726"
FT /id="VSP_001930"
SQ SEQUENCE 417 AA; 46362 MW; B0EF29238CC0C6EB CRC64;
MKATRDHASA PFCTRFNHSD PGIWAAERAV EAPNNLTLPP EPSEDCGSVS VAFSMTMMIT
GFVGNALAIT LVSKSYRRRE GKRKKSFLLC IGWLALTDMV GQLLTSPVVI VLYLSHQRWE
QLDPSGRLCT FFGLTMTVFG LSSLFIASAM AVERALATRA PHWYSSHMKT SVTRAVLLGV
WLAVLAFALL PVLGVGQYTI QWPGTWCFIS TGPGGNGTNS RQNWGNVFFA SAFAILGLSA
LVVTFACNLA TIKALVSRCR AKATASQSSA QWGRITTETA IQLMGIMCVL SVCWSPLLIM
MLKMIFNHTS VEHCKTYTEN QDECNFFLIA VRLASLNQIL DPWVYLLLRK ILLQKFCQLL
KGHSYGLDTE GGTENKDKEM KENLYISNLS RFFILLGHFT EARRGRGHIY LHTLEHQ
