PE2R3_PIG
ID PE2R3_PIG Reviewed; 373 AA.
AC P50131; Q28967;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Prostaglandin E2 receptor EP3 subtype;
DE Short=PGE receptor EP3 subtype;
DE Short=PGE2 receptor EP3 subtype;
DE AltName: Full=Prostanoid EP3 receptor;
GN Name=PTGER3;
OS Sus scrofa (Pig).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX NCBI_TaxID=9823;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Heart;
RA Meyer J., Schroer K.;
RL Submitted (MAY-1995) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 323-365 (ISOFORM SHORT).
RC TISSUE=Heart;
RA Meyer J., Schroer K.;
RL Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Receptor for prostaglandin E2 (PGE2). Required for normal
CC development of fever in response to pyrinogens, including IL1B,
CC prostaglandin E2 and bacterial lipopolysaccharide (LPS). Required for
CC normal potentiation of platelet aggregation by prostaglandin E2, and
CC thus plays a role in the regulation of blood coagulation. Required for
CC increased HCO3(-) secretion in the duodenum in response to mucosal
CC acidification, and thereby contributes to the protection of the mucosa
CC against acid-induced ulceration. Not required for normal kidney
CC function, normal urine volume and osmolality.
CC {ECO:0000250|UniProtKB:P30557}.
CC -!- SUBUNIT: Interacts (via C-terminus) with MKLN1.
CC {ECO:0000250|UniProtKB:P34980}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:P30557};
CC Multi-pass membrane protein {ECO:0000250|UniProtKB:P30557}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=Long;
CC IsoId=P50131-1; Sequence=Displayed;
CC Name=Short;
CC IsoId=P50131-2; Sequence=VSP_001942;
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR EMBL; U27083; AAC63408.1; -; mRNA.
DR EMBL; U30374; AAA75308.1; -; mRNA.
DR RefSeq; NP_998999.1; NM_213834.1. [P50131-1]
DR AlphaFoldDB; P50131; -.
DR SMR; P50131; -.
DR STRING; 9823.ENSSSCP00000004097; -.
DR PaxDb; P50131; -.
DR PRIDE; P50131; -.
DR Ensembl; ENSSSCT00015008308; ENSSSCP00015003345; ENSSSCG00015006184. [P50131-1]
DR Ensembl; ENSSSCT00050026947; ENSSSCP00050011152; ENSSSCG00050019943. [P50131-1]
DR GeneID; 396811; -.
DR KEGG; ssc:396811; -.
DR CTD; 5733; -.
DR eggNOG; KOG3656; Eukaryota.
DR HOGENOM; CLU_045991_3_1_1; -.
DR InParanoid; P50131; -.
DR OrthoDB; 972015at2759; -.
DR TreeFam; TF324982; -.
DR Proteomes; UP000008227; Unplaced.
DR Proteomes; UP000314985; Unplaced.
DR Genevisible; P50131; SS.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR GO; GO:0004957; F:prostaglandin E receptor activity; IBA:GO_Central.
DR GO; GO:0007189; P:adenylate cyclase-activating G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0006954; P:inflammatory response; IBA:GO_Central.
DR GO; GO:0014827; P:intestine smooth muscle contraction; IBA:GO_Central.
DR GO; GO:0060455; P:negative regulation of gastric acid secretion; IBA:GO_Central.
DR GO; GO:0007200; P:phospholipase C-activating G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0007204; P:positive regulation of cytosolic calcium ion concentration; IBA:GO_Central.
DR InterPro; IPR001481; EP3_rcpt_2.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR008365; Prostanoid_rcpt.
DR InterPro; IPR001244; Prostglndn_DP_rcpt.
DR InterPro; IPR000265; Prostglndn_EP3_rcpt.
DR PANTHER; PTHR11866; PTHR11866; 1.
DR PANTHER; PTHR11866:SF10; PTHR11866:SF10; 1.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PRINTS; PR00428; PROSTAGLNDNR.
DR PRINTS; PR01788; PROSTANOIDR.
DR PRINTS; PR00584; PRSTNOIDE32R.
DR PRINTS; PR00582; PRSTNOIDEP3R.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell membrane; Disulfide bond;
KW G-protein coupled receptor; Glycoprotein; Membrane; Receptor;
KW Reference proteome; Transducer; Transmembrane; Transmembrane helix.
FT CHAIN 1..373
FT /note="Prostaglandin E2 receptor EP3 subtype"
FT /id="PRO_0000070060"
FT TOPO_DOM 1..30
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 31..55
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 56..68
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 69..89
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 90..108
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 109..130
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 131..152
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 153..174
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 175..204
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 205..230
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 231..260
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 261..284
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 285..304
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 305..326
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 327..373
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 1..23
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 13
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 194
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 107..185
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
FT VAR_SEQ 337..373
FT /note="AVSQKQREEAATLIFTHLSISRTEPGEARVLFSKSKC -> VANAVCSCSKN
FT GQKVQTISLSHEITQTEA (in isoform Short)"
FT /evidence="ECO:0000303|Ref.2"
FT /id="VSP_001942"
SQ SEQUENCE 373 AA; 41417 MW; 4CB7CC14B6C68186 CRC64;
MWAPERSAEE QGNLTRSLGS SEDCGSVSVV FPMTMLITGF VGNALAMLLV SQSYRRRESK
RKKSFLLCIG WLALTDMVGQ LLTSPVVIVL YLSHQRWEQL DPSGRLCTFF GLTMTAFGLS
SLFIASAMAV ERALAIRAPH WYSSHMKTSA TRAVLLGVWL AVLAFALLPV LGVGQYTIQW
PGTWCFISTR TGGNETSSEN NWGNIFFASA FSFLGLSALV VTFACNLATI KALVSRCRAK
ATASQSSAQW GRITTETAIQ LMGIMCVLSV CWSPLLIMML KTIFNQTSVE YCKVYTEKQN
ECNFFLIAVR LASLNQILDP WVYLLLRKIL LQKFCQAVSQ KQREEAATLI FTHLSISRTE
PGEARVLFSK SKC
