ID   PE35_MYCS2              Reviewed;          97 AA.
AC   A0QNJ3;
DT   30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 1.
DT   25-MAY-2022, entry version 83.
DE   RecName: Full=Uncharacterized PE family protein PE35;
GN   Name=PE35; Synonyms=MsPE35 {ECO:0000303|PubMed:15314236};
GN   OrderedLocusNames=MSMEG_0063, MSMEI_0064;
OS   Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS   smegmatis).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycolicibacterium.
OX   NCBI_TaxID=246196;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RA   Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA   Fraser C.M.;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA   Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA   Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT   "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT   mutations or sequencing errors?";
RL   Genome Biol. 8:R20.1-R20.9(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=18955433; DOI=10.1101/gr.081901.108;
RA   Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA   Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT   "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT   and a new MS-based protocol.";
RL   Genome Res. 19:128-135(2009).
RN   [4]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=15314236; DOI=10.1073/pnas.0404892101;
RA   Flint J.L., Kowalski J.C., Karnati P.K., Derbyshire K.M.;
RT   "The RD1 virulence locus of Mycobacterium tuberculosis regulates DNA
RT   transfer in Mycobacterium smegmatis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:12598-12603(2004).
CC   -!- FUNCTION: Part of the ESX-1 / type VII specialized secretion system
CC       (T7SS), which exports several proteins including EsxA and EsxB
CC       (Probable). Plays a role in DNA conjugation, in at least a donor strain
CC       (PubMed:15314236). {ECO:0000269|PubMed:15314236, ECO:0000305}.
CC   -!- DISRUPTION PHENOTYPE: Increases efficiency of DNA conjugation when
CC       disrupted in donor strain (PubMed:15314236).
CC       {ECO:0000269|PubMed:15314236}.
CC   -!- MISCELLANEOUS: DNA conjugation in M.smegmatis is unidirectional with
CC       distinct donor and recipient strains; mc(2)155 is a donor strain while
CC       MKD8 is a recipient strain. Mutations in a donor strain that alter DNA
CC       transfer do not always alter DNA transfer in a recipient strain.
CC       {ECO:0000305|PubMed:15314236}.
CC   -!- SIMILARITY: Belongs to the mycobacterial PE family. {ECO:0000305}.
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DR   EMBL; CP000480; ABK69830.1; -; Genomic_DNA.
DR   EMBL; CP001663; AFP36546.1; -; Genomic_DNA.
DR   RefSeq; WP_003891390.1; NZ_SIJM01000058.1.
DR   RefSeq; YP_884481.1; NC_008596.1.
DR   AlphaFoldDB; A0QNJ3; -.
DR   SMR; A0QNJ3; -.
DR   STRING; 246196.MSMEI_0064; -.
DR   EnsemblBacteria; ABK69830; ABK69830; MSMEG_0063.
DR   EnsemblBacteria; AFP36546; AFP36546; MSMEI_0064.
DR   GeneID; 66738253; -.
DR   KEGG; msg:MSMEI_0064; -.
DR   KEGG; msm:MSMEG_0063; -.
DR   PATRIC; fig|246196.19.peg.61; -.
DR   eggNOG; ENOG5031PX8; Bacteria.
DR   OMA; MEIAGIY; -.
DR   OrthoDB; 2046595at2; -.
DR   Proteomes; UP000000757; Chromosome.
DR   Proteomes; UP000006158; Chromosome.
DR   InterPro; IPR000084; PE-PGRS_N.
DR   Pfam; PF00934; PE; 1.
PE   3: Inferred from homology;
KW   Reference proteome.
FT   CHAIN           1..97
FT                   /note="Uncharacterized PE family protein PE35"
FT                   /id="PRO_0000438348"
SQ   SEQUENCE   97 AA;  9285 MW;  F4387CEBCBC8019D CRC64;
     MQPMTHNPGA EAVAAQVIAN AARGLAGGTT ASAAVTALVP AGADEVSALA AVAFASEGVE
     ALAANAFAQE ELTRAGAAFA EIAGIYNAVD AANAATM