ID   PE35_MYCTU              Reviewed;          99 AA.
AC   P9WIG7; L0TGV1; Q79F93; Q8VIR8;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 33.
DE   RecName: Full=PE family immunomodulator PE35 {ECO:0000305};
GN   Name=PE35; OrderedLocusNames=Rv3872;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=16030141; DOI=10.1073/pnas.0504922102;
RA   Fortune S.M., Jaeger A., Sarracino D.A., Chase M.R., Sassetti C.M.,
RA   Sherman D.R., Bloom B.R., Rubin E.J.;
RT   "Mutually dependent secretion of proteins required for mycobacterial
RT   virulence.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:10676-10681(2005).
RN   [3]
RP   DISRUPTION PHENOTYPE.
RX   PubMed=16368961; DOI=10.1128/iai.74.1.88-98.2006;
RA   Brodin P., Majlessi L., Marsollier L., de Jonge M.I., Bottai D.,
RA   Demangel C., Hinds J., Neyrolles O., Butcher P.D., Leclerc C., Cole S.T.,
RA   Brosch R.;
RT   "Dissection of ESAT-6 system 1 of Mycobacterium tuberculosis and impact on
RT   immunogenicity and virulence.";
RL   Infect. Immun. 74:88-98(2006).
RN   [4]
RP   IDENTIFICATION AS A POTENTIAL CANDIDATE FOR SERODIAGNOSIS.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=17328726; DOI=10.1111/j.1469-0691.2006.01660.x;
RA   Mukherjee P., Dutta M., Datta P., Dasgupta A., Pradhan R., Pradhan M.,
RA   Kundu M., Basu J., Chakrabarti P.;
RT   "The RD1-encoded antigen Rv3872 of Mycobacterium tuberculosis as a
RT   potential candidate for serodiagnosis of tuberculosis.";
RL   Clin. Microbiol. Infect. 13:146-152(2007).
RN   [5]
RP   IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RC   STRAIN=ATCC 27294 / TMC 102 / H37Rv;
RX   PubMed=17443846; DOI=10.1002/pmic.200600853;
RA   Malen H., Berven F.S., Fladmark K.E., Wiker H.G.;
RT   "Comprehensive analysis of exported proteins from Mycobacterium
RT   tuberculosis H37Rv.";
RL   Proteomics 7:1702-1718(2007).
RN   [6]
RP   FUNCTION, INTERACTION WITH PPE68 AND TLR2, AND SUBCELLULAR LOCATION.
RC   STRAIN=H37Rv;
RX   PubMed=24467650; DOI=10.1111/febs.12723;
RA   Tiwari B., Soory A., Raghunand T.R.;
RT   "An immunomodulatory role for the Mycobacterium tuberculosis region of
RT   difference 1 locus proteins PE35 (Rv3872) and PPE68 (Rv3873).";
RL   FEBS J. 281:1556-1570(2014).
CC   -!- FUNCTION: Plays a major role in RD1-associated pathogenesis, and may
CC       contribute to the establishment and maintenance of M.tuberculosis
CC       infection. Together with PPE68, stimulates the secretion of IL-10 and
CC       MCP-1 from human macrophages, via the interaction with human Toll-like
CC       receptor 2 (TLR2). {ECO:0000269|PubMed:24467650}.
CC   -!- SUBUNIT: Interacts with PPE68. PE35/PPE68 complex interacts with human
CC       TLR2. {ECO:0000269|PubMed:24467650}.
CC   -!- INTERACTION:
CC       P9WIG7; P9WHW9: PPE68; NbExp=3; IntAct=EBI-9086211, EBI-9086224;
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:17443846}. Cell
CC       surface {ECO:0000269|PubMed:24467650}.
CC   -!- DISRUPTION PHENOTYPE: Inactivation impairs expression of EsxA (ESAT-6)
CC       and EsxB (CFP-10). {ECO:0000269|PubMed:16368961}.
CC   -!- MISCELLANEOUS: Could be used for serodiagnosis of both pulmonary and
CC       extra-pulmonary tuberculosis. {ECO:0000269|PubMed:17328726}.
CC   -!- SIMILARITY: Belongs to the mycobacterial PE family. {ECO:0000305}.
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DR   EMBL; AL123456; CCP46701.1; -; Genomic_DNA.
DR   PIR; F70802; F70802.
DR   RefSeq; WP_003912361.1; NZ_NVQJ01000074.1.
DR   RefSeq; YP_178021.1; NC_000962.3.
DR   AlphaFoldDB; P9WIG7; -.
DR   SMR; P9WIG7; -.
DR   IntAct; P9WIG7; 2.
DR   MINT; P9WIG7; -.
DR   STRING; 83332.Rv3872; -.
DR   PaxDb; P9WIG7; -.
DR   DNASU; 886191; -.
DR   GeneID; 886191; -.
DR   KEGG; mtu:Rv3872; -.
DR   TubercuList; Rv3872; -.
DR   eggNOG; ENOG5031UUI; Bacteria.
DR   OMA; MEIAGIY; -.
DR   PhylomeDB; P9WIG7; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0009986; C:cell surface; IEA:UniProtKB-SubCell.
DR   GO; GO:0005576; C:extracellular region; HDA:MTBBASE.
DR   InterPro; IPR000084; PE-PGRS_N.
DR   Pfam; PF00934; PE; 1.
PE   1: Evidence at protein level;
KW   Reference proteome; Secreted; Virulence.
FT   CHAIN           1..99
FT                   /note="PE family immunomodulator PE35"
FT                   /id="PRO_0000394202"
FT   DOMAIN          1..90
FT                   /note="PE"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   99 AA;  9928 MW;  9ABAEB9988A8BFFB CRC64;
     MEKMSHDPIA ADIGTQVSDN ALHGVTAGST ALTSVTGLVP AGADEVSAQA ATAFTSEGIQ
     LLASNASAQD QLHRAGEAVQ DVARTYSQID DGAAGVFAE