PEB1_DROME
ID PEB1_DROME Reviewed; 377 AA.
AC Q9U6L5; Q9W0W1;
DT 28-MAR-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Ejaculatory bulb-specific protein 1;
DE AltName: Full=Ejaculatory bulb-specific protein I;
DE AltName: Full=PEB-me;
DE Flags: Precursor;
GN Name=Ebp {ECO:0000312|FlyBase:FBgn0004181};
GN Synonyms=Peb {ECO:0000312|FlyBase:FBgn0004181};
GN ORFNames=CG2668 {ECO:0000312|FlyBase:FBgn0004181};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|EMBL:AAF47319.2};
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000305}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=10731138; DOI=10.1126/science.287.5461.2222;
RA Rubin G.M., Hong L., Brokstein P., Evans-Holm M., Frise E., Stapleton M.,
RA Harvey D.A.;
RT "A Drosophila complementary DNA resource.";
RL Science 287:2222-2224(2000).
RN [4] {ECO:0000305}
RP PROTEIN SEQUENCE OF 77-90 AND 101-109, FUNCTION, SUBCELLULAR LOCATION, AND
RP TISSUE SPECIFICITY.
RC STRAIN=Canton-S;
RX PubMed=11267893; DOI=10.1016/s0965-1748(00)00154-5;
RA Lung O., Wolfner M.F.;
RT "Identification and characterization of the major Drosophila melanogaster
RT mating plug protein.";
RL Insect Biochem. Mol. Biol. 31:543-551(2001).
CC -!- FUNCTION: Major protein component of the posterior mating plug.
CC Accessory gland proteins constitute, or are required for formation of
CC the anterior mating plug. Posterior mating plug forms before sperm
CC transfer and the anterior mating plug is formed after the start of
CC mating. {ECO:0000269|PubMed:11267893}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:11267893}.
CC -!- TISSUE SPECIFICITY: Specifically expressed in the ejaculatory bulb and
CC seminal fluid. Detected in mated females 3 minutes after the start of
CC mating, and for at least 3 hours after the start of mating.
CC {ECO:0000269|PubMed:11267893}.
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DR EMBL; AE013599; AAF47319.2; -; Genomic_DNA.
DR EMBL; AF184225; AAD55736.1; -; mRNA.
DR RefSeq; NP_001261178.1; NM_001274249.1.
DR RefSeq; NP_611995.1; NM_138151.3.
DR AlphaFoldDB; Q9U6L5; -.
DR BioGRID; 63576; 3.
DR DIP; DIP-19554N; -.
DR IntAct; Q9U6L5; 6.
DR STRING; 7227.FBpp0305377; -.
DR PaxDb; Q9U6L5; -.
DR PRIDE; Q9U6L5; -.
DR DNASU; 38009; -.
DR EnsemblMetazoa; FBtr0072454; FBpp0072356; FBgn0004181.
DR EnsemblMetazoa; FBtr0333174; FBpp0305377; FBgn0004181.
DR GeneID; 38009; -.
DR KEGG; dme:Dmel_CG2668; -.
DR CTD; 10682; -.
DR FlyBase; FBgn0004181; Ebp.
DR VEuPathDB; VectorBase:FBgn0004181; -.
DR eggNOG; ENOG502T8PB; Eukaryota.
DR HOGENOM; CLU_734180_0_0_1; -.
DR InParanoid; Q9U6L5; -.
DR OMA; IGINTPF; -.
DR OrthoDB; 1580312at2759; -.
DR PhylomeDB; Q9U6L5; -.
DR SignaLink; Q9U6L5; -.
DR BioGRID-ORCS; 38009; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 38009; -.
DR PRO; PR:Q9U6L5; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0004181; Expressed in male reproductive gland and 9 other tissues.
DR ExpressionAtlas; Q9U6L5; baseline and differential.
DR Genevisible; Q9U6L5; DM.
DR GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR GO; GO:0005615; C:extracellular space; HDA:FlyBase.
DR GO; GO:0042628; P:mating plug formation; IEP:UniProtKB.
DR GO; GO:0019953; P:sexual reproduction; HEP:FlyBase.
PE 1: Evidence at protein level;
KW Behavior; Direct protein sequencing; Reference proteome; Secreted; Signal.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..377
FT /note="Ejaculatory bulb-specific protein 1"
FT /id="PRO_0000022034"
FT REGION 155..253
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 155..230
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 84
FT /note="G -> GANILAG (in Ref. 4; AA sequence)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 377 AA; 37819 MW; D020F027BD96B221 CRC64;
MVRQLILVLS LILFCGSSHA VVSELARQSE SAIQGLADIK MAPLRYLDVL FGGNPGGLRG
LDGGNSASLS TLQAAKVANI LARGDIASSG YSKISAGVGK GSDLITIIKN TRSYDPYLIP
PGIPGYNYPL GWPLRYPLGP YWPNRPPWLP INSPPIRPGG LFPGGPSPGG PSPGEPSPGE
PSPGGPSPGG PSPGGPSPGG PSPGGPSPGG PSPGGPFPGG SPPSPGGPLG PWQFPWILGG
PRPNRPGRPF PGGILPGHLD GSVVPNSVLN VAGGIFGNGG LFGTGIFGQH GLFGTGFLSG
PSLDPFGIFT PIGNFFGSLG NLFGFSSPSQ IIPIFGGKFG PLGRGLQGSI TLDVGGTVPS
VKGILGQLLH PFLGFLG
