PEB3_DROME
ID PEB3_DROME Reviewed; 126 AA.
AC Q9W1C9; Q23981; Q3LB28;
DT 28-MAR-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 2.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=Ejaculatory bulb-specific protein 3;
DE AltName: Full=Ejaculatory bulb-specific protein III;
DE AltName: Full=PEB-meIII;
DE AltName: Full=PEBmelIII;
DE Flags: Precursor;
GN Name=EbpIII {ECO:0000312|FlyBase:FBgn0011695};
GN Synonyms=PebIII {ECO:0000312|FlyBase:FBgn0011695};
GN ORFNames=CG11390 {ECO:0000312|FlyBase:FBgn0011695};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|EMBL:AAF47140.2};
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=16581978; DOI=10.1093/chemse/bjj050;
RA Zhou J.-J., Kan Y., Antoniw J., Pickett J.A., Field L.M.;
RT "Genome and EST analyses and expression of a gene family with putative
RT functions in insect chemoreception.";
RL Chem. Senses 31:453-465(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3] {ECO:0000305}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [MRNA] OF 6-126, AND TISSUE SPECIFICITY.
RC STRAIN=Oregon-RC; TISSUE=Ejaculatory bulb;
RX PubMed=7619487;
RA Dyanov H.M., Dzitoeva S.G.;
RT "Method for attachment of microscopic preparations on glass for in situ
RT hybridization, PRINS and in situ PCR studies.";
RL BioTechniques 18:822-824(1995).
CC -!- FUNCTION: Protein component of the posterior mating plug.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Specifically expressed in the ejaculatory bulb and
CC seminal fluid. {ECO:0000269|PubMed:7619487}.
CC -!- SIMILARITY: Belongs to the insect A10/OS-D protein family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AJ973478; CAJ01525.1; -; mRNA.
DR EMBL; AE013599; AAF47140.2; -; Genomic_DNA.
DR EMBL; AY113640; AAM29645.1; -; mRNA.
DR EMBL; U08281; AAA87058.1; -; mRNA.
DR RefSeq; NP_001286808.1; NM_001299879.1.
DR RefSeq; NP_001286809.1; NM_001299880.1.
DR RefSeq; NP_524966.1; NM_080227.2.
DR AlphaFoldDB; Q9W1C9; -.
DR SMR; Q9W1C9; -.
DR BioGRID; 72483; 20.
DR DIP; DIP-17564N; -.
DR IntAct; Q9W1C9; 3.
DR STRING; 7227.FBpp0072073; -.
DR PaxDb; Q9W1C9; -.
DR DNASU; 49821; -.
DR EnsemblMetazoa; FBtr0072164; FBpp0072073; FBgn0011695.
DR EnsemblMetazoa; FBtr0343340; FBpp0309998; FBgn0011695.
DR EnsemblMetazoa; FBtr0343341; FBpp0309999; FBgn0011695.
DR GeneID; 49821; -.
DR KEGG; dme:Dmel_CG11390; -.
DR CTD; 49821; -.
DR FlyBase; FBgn0011695; EbpIII.
DR VEuPathDB; VectorBase:FBgn0011695; -.
DR eggNOG; ENOG502S48A; Eukaryota.
DR GeneTree; ENSGT00390000011556; -.
DR HOGENOM; CLU_126727_0_0_1; -.
DR InParanoid; Q9W1C9; -.
DR OMA; LMDQGRC; -.
DR OrthoDB; 1553421at2759; -.
DR PhylomeDB; Q9W1C9; -.
DR BioGRID-ORCS; 49821; 0 hits in 1 CRISPR screen.
DR ChiTaRS; EbpIII; fly.
DR GenomeRNAi; 49821; -.
DR PRO; PR:Q9W1C9; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0011695; Expressed in crop (Drosophila) and 30 other tissues.
DR ExpressionAtlas; Q9W1C9; baseline and differential.
DR Genevisible; Q9W1C9; DM.
DR GO; GO:0005576; C:extracellular region; IDA:FlyBase.
DR GO; GO:0042628; P:mating plug formation; ISS:UniProtKB.
DR GO; GO:0007552; P:metamorphosis; IEP:FlyBase.
DR GO; GO:0009615; P:response to virus; IEP:FlyBase.
DR Gene3D; 1.10.2080.10; -; 1.
DR InterPro; IPR005055; A10/PebIII.
DR InterPro; IPR036682; OS_D_A10/PebIII_sf.
DR PANTHER; PTHR11257; PTHR11257; 1.
DR Pfam; PF03392; OS-D; 1.
DR SUPFAM; SSF100910; SSF100910; 1.
PE 2: Evidence at transcript level;
KW Behavior; Reference proteome; Secreted; Signal.
FT SIGNAL 1..17
FT /evidence="ECO:0000255"
FT CHAIN 18..126
FT /note="Ejaculatory bulb-specific protein 3"
FT /id="PRO_0000022035"
FT CONFLICT 6
FT /note="A -> P (in Ref. 5; AAA87058)"
FT /evidence="ECO:0000305"
FT CONFLICT 95..101
FT /note="PEEWKQL -> ARGVEAA (in Ref. 5; AAA87058)"
FT /evidence="ECO:0000305"
FT CONFLICT 108
FT /note="D -> E (in Ref. 5; AAA87058)"
FT /evidence="ECO:0000305"
FT CONFLICT 117..126
FT /note="ATAEASGIKV -> QPRGSDQGVRRIRFGNTWYMHCIRSTLYLIIKLFLTVG
FT LDLRVWFFP (in Ref. 5; AAA87058)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 126 AA; 14540 MW; 776BF77650F1B14D CRC64;
MKMILALVVL GLVLVAAEDK YTTKYDNIDV DEILKSDRLF GNYFKCLVDN GKCTPEGREL
KKSLPDALKT ECSKCSEKQR QNTDKVIRYI IENKPEEWKQ LQAKYDPDEI YIKRYRATAE
ASGIKV
