ID   PECA_MYCTU              Reviewed;         558 AA.
AC   P9WIF1; L0T8F8; Q10873;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 34.
DE   RecName: Full=PE cleavage protein A {ECO:0000303|PubMed:31662454};
DE   AltName: Full=PE-PGRS family protein PE_PGRS35 {ECO:0000305};
GN   Name=pecA {ECO:0000303|PubMed:31662454}; Synonyms=PE_PGRS35;
GN   OrderedLocusNames=Rv1983; ORFNames=MTCY39.36c;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   FUNCTION, AND PROTEOLYTIC CLEAVAGE.
RX   PubMed=31662454; DOI=10.1128/mbio.01951-19;
RA   Burggraaf M.J., Speer A., Meijers A.S., Ummels R., van der Sar A.M.,
RA   Korotkov K.V., Bitter W., Kuijl C.;
RT   "Type VII secretion substrates of pathogenic Mycobacteria are processed by
RT   a surface protease.";
RL   MBio 10:E01951-E01951(2019).
CC   -!- FUNCTION: Aspartic protease that processes the lipase LipY and other
CC       PE_PGRS proteins. Can also cleave itself.
CC       {ECO:0000269|PubMed:31662454}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:B2HE92}. Cell
CC       surface {ECO:0000250|UniProtKB:B2HE92}. Note=Secreted via the ESX-5 /
CC       type VII secretion system (T7SS). {ECO:0000250|UniProtKB:B2HE92}.
CC   -!- PTM: Undergoes auto-proteolytic processing.
CC       {ECO:0000269|PubMed:31662454}.
CC   -!- SIMILARITY: Belongs to the mycobacterial PE family. PGRS subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AL123456; CCP44753.1; -; Genomic_DNA.
DR   PIR; E70756; E70756.
DR   RefSeq; WP_003899118.1; NZ_KK339370.1.
DR   RefSeq; YP_177854.1; NC_000962.3.
DR   AlphaFoldDB; P9WIF1; -.
DR   SMR; P9WIF1; -.
DR   STRING; 83332.Rv1983; -.
DR   PaxDb; P9WIF1; -.
DR   DNASU; 885921; -.
DR   GeneID; 885921; -.
DR   KEGG; mtu:Rv1983; -.
DR   PATRIC; fig|83332.111.peg.2206; -.
DR   TubercuList; Rv1983; -.
DR   eggNOG; COG0657; Bacteria.
DR   OMA; TPFDAYF; -.
DR   PhylomeDB; P9WIF1; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0009986; C:cell surface; IEA:UniProtKB-SubCell.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004190; F:aspartic-type endopeptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 2.40.70.10; -; 1.
DR   InterPro; IPR000084; PE-PGRS_N.
DR   InterPro; IPR021109; Peptidase_aspartic_dom_sf.
DR   Pfam; PF00934; PE; 1.
PE   1: Evidence at protein level;
KW   Aspartyl protease; Hydrolase; Protease; Reference proteome; Secreted.
FT   CHAIN           1..558
FT                   /note="PE cleavage protein A"
FT                   /id="PRO_0000216166"
FT   DOMAIN          1..93
FT                   /note="PE"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        297
FT                   /evidence="ECO:0000250|UniProtKB:B2HE92"
SQ   SEQUENCE   558 AA;  53738 MW;  17ECBE43778E021E CRC64;
     MSFLVVVPEF LTSAAADVEN IGSTLRAANA AAAASTTALA AAGADEVSAA VAALFARFGQ
     EYQAVSAQAS AFHQQFVQTL NSASGSYAAA EATIASQLQT AQHDLLGAVN APTETLLGRP
     LIGDGAPGTA TSPNGGAGGL LYGNGGNGYS ATASGVGGGA GGSAGLIGNG GAGGAGGPNA
     PGGAGGNGGW LLGNGGIGGP GGASSIPGMS GGAGGTGGAA GLLGWGANGG AGGLGDGVGV
     DRGTGGAGGR GGLLYGGYGV SGPGGDGRTV PLEIIHVTEP TVHANVNGGP TSTILVDTGS
     AGLVVSPEDV GGILGVLHMG LPTGLSISGY SGGLYYIFAT YTTTVDFGNG IVTAPTAVNV
     VLLSIPTSPF AISTYFSALL ADPTTTPFEA YFGAVGVDGV LGVGPNAVGP GPSIPTMALP
     GDLNQGVLID APAGELVFGP NPLPAPNVEV VGSPITTLYV KIDGGTPIPV PSIIDSGGVT
     GTIPSYVIGS GTLPANTNIE VYTSPGGDRL YAFNTNDYRP TVISSGLMNT GFLPFRFQPV
     YIDYSPSGIG TTVFDHPA