PEFA_DICDI
ID PEFA_DICDI Reviewed; 197 AA.
AC Q95YL5; Q54F57; Q95UQ0;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Penta-EF hand domain-containing protein 1;
DE AltName: Full=Apoptosis-linked gene 2 protein homolog A;
DE AltName: Full=Dd-ALG-2a;
DE AltName: Full=DdPEF-1;
GN Name=pefA; Synonyms=alg2A; ORFNames=DDB_G0291081;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE, AND SUBCELLULAR LOCATION.
RC STRAIN=AX2;
RX PubMed=11481037; DOI=10.1093/oxfordjournals.jbchem.a002974;
RA Ohkouchi S., Nishio K., Maeda M., Hitomi K., Adachi H., Maki M.;
RT "Identification and characterization of two penta-EF-hand Ca(2+)-binding
RT proteins in Dictyostelium discoideum.";
RL J. Biochem. 130:207-215(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], SUBUNIT, CALCIUM-BINDING,
RP SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=11927596; DOI=10.1074/jbc.m201718200;
RA Aubry L., Mattei S., Blot B., Sadoul R., Satre M., Klein G.;
RT "Biochemical characterization of two analogues of the apoptosis-linked gene
RT 2 protein in Dictyostelium discoideum and interaction with a physiological
RT partner in mammals, murine Alix.";
RL J. Biol. Chem. 277:21947-21954(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 123-197.
RX PubMed=12198156; DOI=10.1093/emboj/cdf449;
RA Rivero F., Illenberger D., Somesh B.P., Dislich H., Adam N., Meyer A.-K.;
RT "Defects in cytokinesis, actin reorganization and the contractile vacuole
RT in cells deficient in RhoGDI.";
RL EMBO J. 21:4539-4549(2002).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=AX2;
RX PubMed=16926386; DOI=10.1074/mcp.m600113-mcp200;
RA Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M.,
RA Soldati T.;
RT "Proteomics fingerprinting of phagosome maturation and evidence for the
RT role of a Galpha during uptake.";
RL Mol. Cell. Proteomics 5:2228-2243(2006).
CC -!- SUBUNIT: Homodimer; in presence of Ca(2+). May form heterodimers with
CC pefB. {ECO:0000269|PubMed:11927596}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:11481037,
CC ECO:0000269|PubMed:11927596}. Membrane {ECO:0000269|PubMed:11481037,
CC ECO:0000269|PubMed:11927596}; Peripheral membrane protein.
CC Note=Membrane-associated in the presence of Ca(2+).
CC {ECO:0000269|PubMed:11481037}.
CC -!- DEVELOPMENTAL STAGE: Constitutively expressed throughout development
CC (at protein level). Expressed more abundantly in the posterior prespore
CC region of tipped aggregate, slugs and early culminants.
CC {ECO:0000269|PubMed:11481037, ECO:0000269|PubMed:11927596}.
CC -!- DISRUPTION PHENOTYPE: Cells lacking pefA and mutants lacking both pefA
CC and pefB grow normally in axenic medium or on bacterial lawns, and have
CC no major differentiation defect. {ECO:0000269|PubMed:11927596}.
CC -!- SIMILARITY: Belongs to the Peflin/Sorcin family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AB056713; BAB64570.1; -; mRNA.
DR EMBL; AF358911; AAM00237.1; -; mRNA.
DR EMBL; AF358913; AAM00239.1; -; Genomic_DNA.
DR EMBL; AAFI02000175; EAL61888.1; -; Genomic_DNA.
DR EMBL; AY044085; AAK95684.1; -; Genomic_DNA.
DR RefSeq; XP_635397.1; XM_630305.1.
DR AlphaFoldDB; Q95YL5; -.
DR SMR; Q95YL5; -.
DR STRING; 44689.DDB0191092; -.
DR PaxDb; Q95YL5; -.
DR EnsemblProtists; EAL61888; EAL61888; DDB_G0291081.
DR GeneID; 8627981; -.
DR KEGG; ddi:DDB_G0291081; -.
DR dictyBase; DDB_G0291081; pefA.
DR eggNOG; KOG0037; Eukaryota.
DR HOGENOM; CLU_051357_1_1_1; -.
DR InParanoid; Q95YL5; -.
DR PhylomeDB; Q95YL5; -.
DR PRO; PR:Q95YL5; -.
DR Proteomes; UP000002195; Chromosome 5.
DR GO; GO:0005737; C:cytoplasm; IDA:dictyBase.
DR GO; GO:0016020; C:membrane; IDA:dictyBase.
DR GO; GO:0045335; C:phagocytic vesicle; HDA:dictyBase.
DR GO; GO:0005509; F:calcium ion binding; IDA:dictyBase.
DR GO; GO:0048306; F:calcium-dependent protein binding; IPI:dictyBase.
DR GO; GO:0042802; F:identical protein binding; IPI:dictyBase.
DR GO; GO:0009617; P:response to bacterium; HEP:dictyBase.
DR GO; GO:0009611; P:response to wounding; IDA:dictyBase.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR Pfam; PF13202; EF-hand_5; 1.
DR Pfam; PF13499; EF-hand_7; 1.
DR SMART; SM00054; EFh; 3.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS00018; EF_HAND_1; 3.
DR PROSITE; PS50222; EF_HAND_2; 3.
PE 1: Evidence at protein level;
KW Calcium; Cytoplasm; Membrane; Metal-binding; Reference proteome; Repeat.
FT CHAIN 1..197
FT /note="Penta-EF hand domain-containing protein 1"
FT /id="PRO_0000328612"
FT DOMAIN 33..67
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 68..103
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 111..133
FT /note="EF-hand 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 46
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 48
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 50
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 52
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 57
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 81
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 83
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 85
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 87
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 92
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 111
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 113
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 115
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 117
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 122
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
SQ SEQUENCE 197 AA; 22355 MW; 9562C60595C46066 CRC64;
MMSYGYQQTP IVAPPAKFQA LWFYSFYTQI QHQQLHEMSA WFRSIDKDRS GSISAMELQH
LHVGYGPLGI ETATKLIRVF DVDKSGQIDF YEYAALHQFI NILYANFLAN DRNRSGTIDA
QEIHRALGTS GFNLPFNTVN LLFLKASPRG YGLKFSDFLG LCASIAIARS LFEAHDRMRT
GVAHLNVNHI YDIISLV
