ID   PELB_ASPNC              Reviewed;         379 AA.
AC   A2QFN7;
DT   18-MAY-2010, integrated into UniProtKB/Swiss-Prot.
DT   06-MAR-2007, sequence version 1.
DT   25-MAY-2022, entry version 67.
DE   RecName: Full=Probable pectin lyase B;
DE            Short=PLB;
DE            EC=4.2.2.10;
DE   Flags: Precursor;
GN   Name=pelB; ORFNames=An03g00190;
OS   Aspergillus niger (strain CBS 513.88 / FGSC A1513).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Circumdati.
OX   NCBI_TaxID=425011;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CBS 513.88 / FGSC A1513 / ATCC MYA-4892;
RX   PubMed=17259976; DOI=10.1038/nbt1282;
RA   Pel H.J., de Winde J.H., Archer D.B., Dyer P.S., Hofmann G., Schaap P.J.,
RA   Turner G., de Vries R.P., Albang R., Albermann K., Andersen M.R.,
RA   Bendtsen J.D., Benen J.A.E., van den Berg M., Breestraat S., Caddick M.X.,
RA   Contreras R., Cornell M., Coutinho P.M., Danchin E.G.J., Debets A.J.M.,
RA   Dekker P., van Dijck P.W.M., van Dijk A., Dijkhuizen L., Driessen A.J.M.,
RA   d'Enfert C., Geysens S., Goosen C., Groot G.S.P., de Groot P.W.J.,
RA   Guillemette T., Henrissat B., Herweijer M., van den Hombergh J.P.T.W.,
RA   van den Hondel C.A.M.J.J., van der Heijden R.T.J.M., van der Kaaij R.M.,
RA   Klis F.M., Kools H.J., Kubicek C.P., van Kuyk P.A., Lauber J., Lu X.,
RA   van der Maarel M.J.E.C., Meulenberg R., Menke H., Mortimer M.A.,
RA   Nielsen J., Oliver S.G., Olsthoorn M., Pal K., van Peij N.N.M.E.,
RA   Ram A.F.J., Rinas U., Roubos J.A., Sagt C.M.J., Schmoll M., Sun J.,
RA   Ussery D., Varga J., Vervecken W., van de Vondervoort P.J.J., Wedler H.,
RA   Woesten H.A.B., Zeng A.-P., van Ooyen A.J.J., Visser J., Stam H.;
RT   "Genome sequencing and analysis of the versatile cell factory Aspergillus
RT   niger CBS 513.88.";
RL   Nat. Biotechnol. 25:221-231(2007).
CC   -!- FUNCTION: Pectinolytic enzymes consist of four classes of enzymes:
CC       pectin lyase, polygalacturonase, pectin methylesterase and
CC       rhamnogalacturonase. Among pectinolytic enzymes, pectin lyase is the
CC       most important in depolymerization of pectin, since it cleaves internal
CC       glycosidic bonds of highly methylated pectins (By similarity).
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Eliminative cleavage of (1->4)-alpha-D-galacturonan methyl
CC         ester to give oligosaccharides with 4-deoxy-6-O-methyl-alpha-D-
CC         galact-4-enuronosyl groups at their non-reducing ends.; EC=4.2.2.10;
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the polysaccharide lyase 1 family.
CC       {ECO:0000305}.
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DR   EMBL; AM270043; CAK37997.1; -; Genomic_DNA.
DR   RefSeq; XP_001389926.1; XM_001389889.1.
DR   AlphaFoldDB; A2QFN7; -.
DR   SMR; A2QFN7; -.
DR   CAZy; PL1; Polysaccharide Lyase Family 1.
DR   PaxDb; A2QFN7; -.
DR   EnsemblFungi; CAK37997; CAK37997; An03g00190.
DR   GeneID; 4980695; -.
DR   KEGG; ang:ANI_1_16034; -.
DR   VEuPathDB; FungiDB:An03g00190; -.
DR   HOGENOM; CLU_021980_0_1_1; -.
DR   Proteomes; UP000006706; Chromosome 6R.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0030570; F:pectate lyase activity; IEA:InterPro.
DR   GO; GO:0047490; F:pectin lyase activity; ISS:UniProtKB.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR   GO; GO:0045490; P:pectin catabolic process; ISS:UniProtKB.
DR   Gene3D; 2.160.20.10; -; 1.
DR   InterPro; IPR002022; Pec_lyase.
DR   InterPro; IPR012334; Pectin_lyas_fold.
DR   InterPro; IPR011050; Pectin_lyase_fold/virulence.
DR   InterPro; IPR045032; PEL.
DR   PANTHER; PTHR31683; PTHR31683; 1.
DR   Pfam; PF00544; Pectate_lyase_4; 1.
DR   SMART; SM00656; Amb_all; 1.
DR   SUPFAM; SSF51126; SSF51126; 1.
PE   3: Inferred from homology;
KW   Carbohydrate metabolism; Cell wall biogenesis/degradation; Disulfide bond;
KW   Glycoprotein; Lyase; Polysaccharide degradation; Reference proteome;
KW   Secreted; Signal.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   CHAIN           21..379
FT                   /note="Probable pectin lyase B"
FT                   /id="PRO_5000219710"
FT   ACT_SITE        256
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        129
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        252
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        83..102
FT                   /evidence="ECO:0000250"
FT   DISULFID        92..226
FT                   /evidence="ECO:0000250"
FT   DISULFID        323..331
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   379 AA;  39854 MW;  FC398DC1C935F222 CRC64;
     MHYKLLFAAA AASLASAVSA AGVVGAAEGF AHGVTGGGSA SPVYPTTTDE LVSYLGDNEP
     RVIILDRTFD FTGTEGTETT TGCAPWGTAS QCQVAINLHS WCDNYQASAP KVSVTYDKAG
     ILPITVNSNK SIVGQGTKGV IKGKGLRVVS GAKNVIIQNI AVTDINPKYV WGGDAITVDD
     SDLVWIDHVT TARIGRQHIV LGTSADNRVT ISYSLIDGRS DYSATCNGHH YWGVYLDGSN
     DMVTLKGNYF YNLSGRMPKV QGNTLLHAVN NLFHNFDGHA FEIGTGGYVL AEGNVFQDVN
     TVVETPISGQ LFSSPDANTN QQCASVFGRS CQLNAFGNSG SMSGSDTSII SKFAGKTIAA
     AHPPGNIAQW TMKNAGQGK