ID   PELD_PSEMA              Reviewed;         312 AA.
AC   P27027;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   25-MAY-2022, entry version 72.
DE   RecName: Full=Pectin lyase;
DE            EC=4.2.2.10;
GN   Name=pnl;
OS   Pseudomonas marginalis.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=298;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 2-34.
RC   STRAIN=N6301;
RX   PubMed=1731776; DOI=10.1016/s0006-291x(05)80105-6;
RA   Nikaidou N., Kamio Y., Izaki K.;
RT   "Molecular cloning and nucleotide sequence of a pectin lyase gene from
RT   Pseudomonas marginalis N6301.";
RL   Biochem. Biophys. Res. Commun. 182:14-19(1992).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Eliminative cleavage of (1->4)-alpha-D-galacturonan methyl
CC         ester to give oligosaccharides with 4-deoxy-6-O-methyl-alpha-D-
CC         galact-4-enuronosyl groups at their non-reducing ends.; EC=4.2.2.10;
CC   -!- INDUCTION: By DNA-damaging agents such as mitomycin C.
CC   -!- SIMILARITY: Belongs to the polysaccharide lyase 1 family.
CC       {ECO:0000305}.
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DR   EMBL; D32121; BAA06847.1; -; Genomic_DNA.
DR   PIR; JQ1318; JQ1318.
DR   AlphaFoldDB; P27027; -.
DR   SMR; P27027; -.
DR   CAZy; PL1; Polysaccharide Lyase Family 1.
DR   GO; GO:0030570; F:pectate lyase activity; IEA:InterPro.
DR   GO; GO:0047490; F:pectin lyase activity; IEA:UniProtKB-EC.
DR   Gene3D; 2.160.20.10; -; 1.
DR   InterPro; IPR002022; Pec_lyase.
DR   InterPro; IPR012334; Pectin_lyas_fold.
DR   InterPro; IPR011050; Pectin_lyase_fold/virulence.
DR   InterPro; IPR045032; PEL.
DR   PANTHER; PTHR31683; PTHR31683; 1.
DR   Pfam; PF00544; Pectate_lyase_4; 1.
DR   SMART; SM00656; Amb_all; 1.
DR   SUPFAM; SSF51126; SSF51126; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Lyase.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:1731776"
FT   CHAIN           2..312
FT                   /note="Pectin lyase"
FT                   /id="PRO_0000212996"
FT   REGION          254..274
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        201
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   312 AA;  34103 MW;  0A5CFF5610A09D45 CRC64;
     MSYPESKLTG LTGFALAAKV TGGWAGPVVS ITNLDQLKAN IGTVTPQVLV INSNISASSL
     TKVNMGANKT LIGSFQNRTL ENIHLRATAQ SQNIILQNLI FKHSANIKAN DDIQVYLNYG
     SKYWIDHCSF VGHSWSTTDG SEDKLLYIGE KADYATISNC FFGSHKYGLI FGHPADDNNA
     AFNGYPRLTL CHNRFDNMEV RAPGLMRYGY FHVYNNYINK FHLGFTLAQN ANILSESNYF
     GEGSQNNGML DDKGSGTFTD TNSVPPITNQ KSPKAQWTAT SNYAYTLKTA AQAKDFTQKN
     AGAQAAALVF GS