PELO_AERPE
ID PELO_AERPE Reviewed; 356 AA.
AC Q9YAZ5;
DT 10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT 14-NOV-2006, sequence version 2.
DT 25-MAY-2022, entry version 106.
DE RecName: Full=Protein pelota homolog {ECO:0000255|HAMAP-Rule:MF_01853};
DE EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_01853};
GN Name=pelA {ECO:0000255|HAMAP-Rule:MF_01853}; OrderedLocusNames=APE_1800.1;
OS Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 /
OS K1).
OC Archaea; Crenarchaeota; Thermoprotei; Desulfurococcales;
OC Desulfurococcaceae; Aeropyrum.
OX NCBI_TaxID=272557;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1;
RX PubMed=10382966; DOI=10.1093/dnares/6.2.83;
RA Kawarabayasi Y., Hino Y., Horikawa H., Yamazaki S., Haikawa Y., Jin-no K.,
RA Takahashi M., Sekine M., Baba S., Ankai A., Kosugi H., Hosoyama A.,
RA Fukui S., Nagai Y., Nishijima K., Nakazawa H., Takamiya M., Masuda S.,
RA Funahashi T., Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A.,
RA Aoki K., Kubota K., Nakamura Y., Nomura N., Sako Y., Kikuchi H.;
RT "Complete genome sequence of an aerobic hyper-thermophilic crenarchaeon,
RT Aeropyrum pernix K1.";
RL DNA Res. 6:83-101(1999).
CC -!- FUNCTION: May function in recognizing stalled ribosomes, interact with
CC stem-loop structures in stalled mRNA molecules, and effect
CC endonucleolytic cleavage of the mRNA. May play a role in the release
CC non-functional ribosomes and degradation of damaged mRNAs. Has
CC endoribonuclease activity. {ECO:0000255|HAMAP-Rule:MF_01853}.
CC -!- COFACTOR:
CC Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01853};
CC -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_01853}.
CC -!- INTERACTION:
CC Q9YAZ5; Q9YAV0: tuf; NbExp=3; IntAct=EBI-15880754, EBI-15880729;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01853}.
CC -!- DOMAIN: The N-terminal domain has the RNA-binding Sm fold. It harbors
CC the endoribonuclease activity. {ECO:0000255|HAMAP-Rule:MF_01853}.
CC -!- SIMILARITY: Belongs to the eukaryotic release factor 1 family. Pelota
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01853}.
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DR EMBL; BA000002; BAA80803.2; -; Genomic_DNA.
DR PIR; F72564; F72564.
DR PDB; 3WXM; X-ray; 2.30 A; B/D/F/H=1-356.
DR PDB; 6JI2; X-ray; 3.00 A; B/F=1-356.
DR PDBsum; 3WXM; -.
DR PDBsum; 6JI2; -.
DR AlphaFoldDB; Q9YAZ5; -.
DR SMR; Q9YAZ5; -.
DR DIP; DIP-59400N; -.
DR IntAct; Q9YAZ5; 1.
DR STRING; 272557.APE_1800.1; -.
DR EnsemblBacteria; BAA80803; BAA80803; APE_1800.1.
DR KEGG; ape:APE_1800.1; -.
DR eggNOG; arCOG01741; Archaea.
DR OMA; DDLWHLK; -.
DR Proteomes; UP000002518; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0070966; P:nuclear-transcribed mRNA catabolic process, no-go decay; IEA:InterPro.
DR GO; GO:0070481; P:nuclear-transcribed mRNA catabolic process, non-stop decay; IEA:InterPro.
DR GO; GO:0071025; P:RNA surveillance; IEA:InterPro.
DR Gene3D; 2.30.30.870; -; 1.
DR Gene3D; 3.30.1330.30; -; 1.
DR Gene3D; 3.30.420.60; -; 1.
DR HAMAP; MF_01853; PelO; 1.
DR InterPro; IPR042226; eFR1_2_sf.
DR InterPro; IPR005140; eRF1_1_Pelota.
DR InterPro; IPR005142; eRF1_3.
DR InterPro; IPR029064; L30e-like.
DR InterPro; IPR038069; Pelota/DOM34_N.
DR InterPro; IPR023521; Pelota_arc.
DR InterPro; IPR004405; Transl-rel_pelota.
DR PANTHER; PTHR10853; PTHR10853; 1.
DR Pfam; PF03463; eRF1_1; 1.
DR Pfam; PF03465; eRF1_3; 1.
DR SMART; SM01194; eRF1_1; 1.
DR SUPFAM; SSF159065; SSF159065; 1.
DR SUPFAM; SSF55315; SSF55315; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Endonuclease; Hydrolase; Metal-binding; Nuclease;
KW Reference proteome.
FT CHAIN 1..356
FT /note="Protein pelota homolog"
FT /id="PRO_0000361779"
FT STRAND 2..5
FT /evidence="ECO:0007829|PDB:3WXM"
FT STRAND 12..16
FT /evidence="ECO:0007829|PDB:3WXM"
FT HELIX 20..29
FT /evidence="ECO:0007829|PDB:3WXM"
FT STRAND 35..44
FT /evidence="ECO:0007829|PDB:3WXM"
FT STRAND 47..49
FT /evidence="ECO:0007829|PDB:3WXM"
FT STRAND 52..67
FT /evidence="ECO:0007829|PDB:3WXM"
FT STRAND 73..82
FT /evidence="ECO:0007829|PDB:3WXM"
FT HELIX 85..87
FT /evidence="ECO:0007829|PDB:3WXM"
FT STRAND 92..99
FT /evidence="ECO:0007829|PDB:3WXM"
FT STRAND 104..107
FT /evidence="ECO:0007829|PDB:3WXM"
FT HELIX 114..122
FT /evidence="ECO:0007829|PDB:3WXM"
FT STRAND 128..134
FT /evidence="ECO:0007829|PDB:3WXM"
FT STRAND 136..145
FT /evidence="ECO:0007829|PDB:3WXM"
FT STRAND 148..156
FT /evidence="ECO:0007829|PDB:3WXM"
FT HELIX 167..189
FT /evidence="ECO:0007829|PDB:3WXM"
FT STRAND 192..199
FT /evidence="ECO:0007829|PDB:3WXM"
FT HELIX 202..213
FT /evidence="ECO:0007829|PDB:3WXM"
FT STRAND 217..223
FT /evidence="ECO:0007829|PDB:3WXM"
FT HELIX 228..235
FT /evidence="ECO:0007829|PDB:3WXM"
FT HELIX 239..244
FT /evidence="ECO:0007829|PDB:3WXM"
FT TURN 245..247
FT /evidence="ECO:0007829|PDB:3WXM"
FT HELIX 249..266
FT /evidence="ECO:0007829|PDB:3WXM"
FT HELIX 268..270
FT /evidence="ECO:0007829|PDB:3WXM"
FT STRAND 271..274
FT /evidence="ECO:0007829|PDB:3WXM"
FT HELIX 275..284
FT /evidence="ECO:0007829|PDB:3WXM"
FT STRAND 287..293
FT /evidence="ECO:0007829|PDB:3WXM"
FT HELIX 294..298
FT /evidence="ECO:0007829|PDB:3WXM"
FT HELIX 302..317
FT /evidence="ECO:0007829|PDB:3WXM"
FT STRAND 321..325
FT /evidence="ECO:0007829|PDB:3WXM"
FT STRAND 327..329
FT /evidence="ECO:0007829|PDB:3WXM"
FT HELIX 330..335
FT /evidence="ECO:0007829|PDB:3WXM"
FT HELIX 336..338
FT /evidence="ECO:0007829|PDB:3WXM"
FT STRAND 341..347
FT /evidence="ECO:0007829|PDB:3WXM"
FT HELIX 351..353
FT /evidence="ECO:0007829|PDB:3WXM"
SQ SEQUENCE 356 AA; 39238 MW; 389A5D27B7752986 CRC64;
MRVEVLDNKR RIVRLRPESE EDLWLLRITL RPGDVVRIRT SRDVPVGSGR KERVVMTLRI
RLDSIEFQPF TGKLRISGIV VEGPDEFGVK GRRHSTAVSI GTWLVVERDK GWSEQELERL
ASGRARGTAV IAAVDYDEFA LAVLAGHGMK ILEDTSARLP GKDDPSREQE VEKYVDRAAK
RIVEEAARHR SPIAVIAGPG QLKTSVAEKV QRAMPSLKVA TVDTSMGGVA GVREALRRES
VTRILRELSI VEAEGVLEEF LRRIAKSRDT VAYTPGEVLA VARMGAVDTV LLVDTLLHSP
DDAVREAVDE ALRLVESMGG RVIIIPGDSP AGERLVSFGG VIALLRYPVP QEARRL
