PEN2_DICDI
ID PEN2_DICDI Reviewed; 97 AA.
AC Q54BR1;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 08-APR-2008, sequence version 2.
DT 25-MAY-2022, entry version 64.
DE RecName: Full=Probable gamma-secretase subunit PEN-2;
GN Name=psenen; ORFNames=DDB_G0293484;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=10048482; DOI=10.1093/dnares/5.6.335;
RA Morio T., Urushihara H., Saito T., Ugawa Y., Mizuno H., Yoshida M.,
RA Yoshino R., Mitra B.N., Pi M., Sato T., Takemoto K., Yasukawa H.,
RA Williams J., Maeda M., Takeuchi I., Ochiai H., Tanaka Y.;
RT "The Dictyostelium developmental cDNA project: generation and analysis of
RT expressed sequence tags from the first-finger stage of development.";
RL DNA Res. 5:335-340(1998).
CC -!- FUNCTION: Essential subunit of the gamma-secretase complex, an
CC endoprotease complex that catalyzes the intramembrane cleavage of
CC integral membrane proteins such as Notch receptors. The gamma-secretase
CC complex plays a role in Notch and Wnt signaling cascades and regulation
CC of downstream processes via its role in processing key regulatory
CC proteins. {ECO:0000250|UniProtKB:Q9NZ42}.
CC -!- SUBUNIT: The functional gamma-secretase complex is composed of at least
CC four polypeptides: a presenilin homodimer, nicastrin, aph1 and psenen.
CC {ECO:0000250|UniProtKB:Q9NZ42}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000250|UniProtKB:Q9NZ42}; Multi-pass membrane protein
CC {ECO:0000250|UniProtKB:Q9NZ42}. Golgi apparatus, Golgi stack membrane
CC {ECO:0000250|UniProtKB:Q9NZ42}; Multi-pass membrane protein
CC {ECO:0000250|UniProtKB:Q9NZ42}. Cell membrane
CC {ECO:0000250|UniProtKB:Q9NZ42}; Multi-pass membrane protein
CC {ECO:0000250|UniProtKB:Q9NZ42}. Membrane
CC {ECO:0000250|UniProtKB:Q9NZ42}; Multi-pass membrane protein
CC {ECO:0000250|UniProtKB:Q9NZ42}. Note=Predominantly located in the
CC endoplasmic reticulum and in the cis-Golgi.
CC {ECO:0000250|UniProtKB:Q9NZ42}.
CC -!- SIMILARITY: Belongs to the PEN-2 family. {ECO:0000305}.
CC -!- CAUTION: 3D-structure analysis of the human homolog indicates that the
CC membrane topology differs from the predictions. Contrary to
CC predictions, the N-terminus contains two short helices that dip into
CC the membrane, but do not cross it. The C-terminus contains the single
CC transmembrane helix. This gives rise to a topology where the N-terminus
CC is cytoplasmic and the C-terminus is lumenal. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AAFI02000213; EAL60704.1; -; Genomic_DNA.
DR EMBL; C92437; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_629118.1; XM_629116.1.
DR AlphaFoldDB; Q54BR1; -.
DR SMR; Q54BR1; -.
DR STRING; 44689.DDB0266398; -.
DR PaxDb; Q54BR1; -.
DR EnsemblProtists; EAL60704; EAL60704; DDB_G0293484.
DR GeneID; 8629248; -.
DR KEGG; ddi:DDB_G0293484; -.
DR dictyBase; DDB_G0293484; psenen.
DR eggNOG; KOG3402; Eukaryota.
DR InParanoid; Q54BR1; -.
DR PhylomeDB; Q54BR1; -.
DR PRO; PR:Q54BR1; -.
DR Proteomes; UP000002195; Chromosome 6.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0070765; C:gamma-secretase complex; IBA:GO_Central.
DR GO; GO:0032580; C:Golgi cisterna membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0007219; P:Notch signaling pathway; IEA:UniProtKB-KW.
DR InterPro; IPR019379; Gamma_Secretase_Asp_P_PEN2.
DR PANTHER; PTHR16318; PTHR16318; 1.
DR Pfam; PF10251; PEN-2; 1.
PE 3: Inferred from homology;
KW Cell membrane; Endoplasmic reticulum; Golgi apparatus; Membrane;
KW Notch signaling pathway; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..97
FT /note="Probable gamma-secretase subunit PEN-2"
FT /id="PRO_0000328268"
FT TOPO_DOM 1..20
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q9NZ42"
FT INTRAMEM 21..39
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:Q9NZ42"
FT TOPO_DOM 40..55
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q9NZ42"
FT TRANSMEM 56..76
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:Q9NZ42"
FT TOPO_DOM 77..97
FT /note="Lumenal"
FT /evidence="ECO:0000250|UniProtKB:Q9NZ42"
FT CONFLICT 30
FT /note="P -> T (in Ref. 1; EAL60704)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 97 AA; 11585 MW; F9CF2280E92F9625 CRC64;
MLIPEDDKLD DEKMINIAKK LWFIGFFFLP WVWLINILYF IPYRNSLNDK VKWYLKFSLI
GFLGYSTIFM GWMGIYLVNR NKWGAFGDDI SITIPFG
