PEN3_ARATH
ID PEN3_ARATH Reviewed; 760 AA.
AC Q9LVY2;
DT 03-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 22-SEP-2009, sequence version 2.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Tirucalladienol synthase;
DE EC=5.4.99.56;
DE AltName: Full=Pentacyclic triterpene synthase 3;
DE Short=AtPEN3;
GN Name=PEN3; OrderedLocusNames=At5g36150; ORFNames=MAB16.10;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:31-63(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP IDENTIFICATION, AND NOMENCLATURE.
RX PubMed=11247608; DOI=10.1023/a:1006476123930;
RA Husselstein-Muller T., Schaller H., Benveniste P.;
RT "Molecular cloning and expression in yeast of 2,3-oxidosqualene-
RT triterpenoid cyclases from Arabidopsis thaliana.";
RL Plant Mol. Biol. 45:75-92(2001).
RN [4]
RP FUNCTION, AND CATALYTIC ACTIVITY.
RX PubMed=19445469; DOI=10.1021/ol9005745;
RA Morlacchi P., Wilson W.K., Xiong Q., Bhaduri A., Sttivend D.,
RA Kolesnikova M.D., Matsuda S.P.T.;
RT "Product profile of PEN3: the last unexamined oxidosqualene cyclase in
RT Arabidopsis thaliana.";
RL Org. Lett. 11:2627-2630(2009).
CC -!- FUNCTION: Converts oxidosqualene to tirucalladienol.
CC {ECO:0000269|PubMed:19445469}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(S)-2,3-epoxysqualene = tirucalladienol; Xref=Rhea:RHEA:31887,
CC ChEBI:CHEBI:15441, ChEBI:CHEBI:63468; EC=5.4.99.56;
CC Evidence={ECO:0000269|PubMed:19445469};
CC -!- SIMILARITY: Belongs to the terpene cyclase/mutase family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAA96890.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB018112; BAA96890.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED94049.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM70931.1; -; Genomic_DNA.
DR RefSeq; NP_001318680.1; NM_001344127.1.
DR RefSeq; NP_198464.4; NM_123006.4.
DR AlphaFoldDB; Q9LVY2; -.
DR SMR; Q9LVY2; -.
DR STRING; 3702.AT5G36150.1; -.
DR iPTMnet; Q9LVY2; -.
DR PaxDb; Q9LVY2; -.
DR PRIDE; Q9LVY2; -.
DR ProteomicsDB; 235113; -.
DR EnsemblPlants; AT5G36150.1; AT5G36150.1; AT5G36150.
DR EnsemblPlants; AT5G36150.2; AT5G36150.2; AT5G36150.
DR GeneID; 833612; -.
DR Gramene; AT5G36150.1; AT5G36150.1; AT5G36150.
DR Gramene; AT5G36150.2; AT5G36150.2; AT5G36150.
DR KEGG; ath:AT5G36150; -.
DR Araport; AT5G36150; -.
DR TAIR; locus:2158921; AT5G36150.
DR eggNOG; KOG0497; Eukaryota.
DR HOGENOM; CLU_009074_2_0_1; -.
DR InParanoid; Q9LVY2; -.
DR OMA; ESHSFMF; -.
DR OrthoDB; 365003at2759; -.
DR PhylomeDB; Q9LVY2; -.
DR BioCyc; ARA:AT5G36150-MON; -.
DR BioCyc; MetaCyc:AT5G36150-MON; -.
DR PRO; PR:Q9LVY2; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LVY2; baseline and differential.
DR Genevisible; Q9LVY2; AT.
DR GO; GO:0005811; C:lipid droplet; IEA:InterPro.
DR GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR GO; GO:0042300; F:beta-amyrin synthase activity; IBA:GO_Central.
DR GO; GO:0042299; F:lupeol synthase activity; ISS:TAIR.
DR GO; GO:0016104; P:triterpenoid biosynthetic process; IBA:GO_Central.
DR CDD; cd02892; SQCY_1; 1.
DR InterPro; IPR032696; SQ_cyclase_C.
DR InterPro; IPR032697; SQ_cyclase_N.
DR InterPro; IPR018333; Squalene_cyclase.
DR InterPro; IPR002365; Terpene_synthase_CS.
DR InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR PANTHER; PTHR11764; PTHR11764; 1.
DR Pfam; PF13243; SQHop_cyclase_C; 1.
DR Pfam; PF13249; SQHop_cyclase_N; 1.
DR SUPFAM; SSF48239; SSF48239; 2.
DR TIGRFAMs; TIGR01787; squalene_cyclas; 1.
DR PROSITE; PS01074; TERPENE_SYNTHASES; 1.
PE 1: Evidence at protein level;
KW Isomerase; Reference proteome; Repeat.
FT CHAIN 1..760
FT /note="Tirucalladienol synthase"
FT /id="PRO_0000366138"
FT REPEAT 149..190
FT /note="PFTB 1"
FT REPEAT 641..682
FT /note="PFTB 2"
FT ACT_SITE 486
FT /note="Proton donor"
FT /evidence="ECO:0000250|UniProtKB:P48449"
SQ SEQUENCE 760 AA; 87804 MW; D30F08C6501FCE4B CRC64;
MWRLRIGAKA GDDPHLCTTN NFLGRQIWEF DANAGSPAEL SEVDQARQNF SNNRSQYKAC
ADLLWRMQFL REKNFEQKIP RVRIEDAKKI TFEDAKNTLR RGIHYMAALQ SDDGHWPSEN
AGCIFFNAPF VICLYITGHL DKVFSEEHRK EMLRYMYNHQ NDDGGWGIDV ESHSFMFCTV
INYICLRIFG VDPDHDGESA CARARKWIID HGGATYTPLF GKAWLSVLGV YEWSGCKPIP
PEFWFFPSYF PINGGTLWIY LRDTFMAMSY LYGKKFVAKP TPLILQLREE LYPQPYAEIV
WSQARSRCAK EDLYYPQSLV QDLFWKLVHM FSENILNRWP FNKLIREKAI RTAMELIHYH
DEATRYITGG AVPKVFHMLA CWVEDPESDY FKKHLARVSH FIWIAEDGLK IQTFGSQIWD
TAFVLQVMLA ADVDDEIRPT LIKGYSYLRK SQFTENPPGD YINMFRDISK GGWGYSDKDQ
GWPVSDCISE SLECCLIFES MSSEFIGEKM EVERLYDAVN MLLYMQSRNG GISIWEAASG
KKWLEWLSPI EFIEDTILEH EYLECTGSAI VVLARFMKQF PGHRTEEVKK FITKGVKYIE
SLQIADGSWY GNWGICFIYG TFFAVRGLVA AGNTYDNCEA IRRAVRFLLD IQNGEGGWGE
SFLSCPNKNY IPLEGNKTDV VNTGQALMVL IMGGQMDRDP LPVHRAAKVL INSQMDNGDF
PQQEIRGVYK MNVMLNFPTF RNSFTLWALT HYTKAIRLLL
