PEN5_ARATH
ID PEN5_ARATH Reviewed; 761 AA.
AC Q9FJV8;
DT 03-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Marneral synthase;
DE Short=AtMRN1;
DE EC=5.4.99.53;
DE AltName: Full=Pentacyclic triterpene synthase 5;
DE Short=AtPEN5;
GN Name=MRN1; Synonyms=PEN5; OrderedLocusNames=At5g42600; ORFNames=MFO20.1;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9734815; DOI=10.1093/dnares/5.3.203;
RA Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence
RT features of the regions of 1,367,185 bp covered by 19 physically assigned
RT P1 and TAC clones.";
RL DNA Res. 5:203-216(1998).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Cheuk R.F., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP IDENTIFICATION, AND NOMENCLATURE.
RX PubMed=11247608; DOI=10.1023/a:1006476123930;
RA Husselstein-Muller T., Schaller H., Benveniste P.;
RT "Molecular cloning and expression in yeast of 2,3-oxidosqualene-
RT triterpenoid cyclases from Arabidopsis thaliana.";
RL Plant Mol. Biol. 45:75-92(2001).
RN [5]
RP FUNCTION, AND CATALYTIC ACTIVITY.
RX PubMed=16425307; DOI=10.1002/anie.200503420;
RA Xiong Q., Wilson W.K., Matsuda S.P.T.;
RT "An Arabidopsis oxidosqualene cyclase catalyzes iridal skeleton formation
RT by Grob fragmentation.";
RL Angew. Chem. Int. Ed. 45:1285-1288(2006).
CC -!- FUNCTION: Converts oxidosqualene to marneral.
CC {ECO:0000269|PubMed:16425307}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(S)-2,3-epoxysqualene = marneral; Xref=Rhea:RHEA:31875,
CC ChEBI:CHEBI:15441, ChEBI:CHEBI:63465; EC=5.4.99.53;
CC Evidence={ECO:0000269|PubMed:16425307};
CC -!- SIMILARITY: Belongs to the terpene cyclase/mutase family.
CC {ECO:0000305}.
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DR EMBL; AB013391; BAB10498.1; -; Genomic_DNA.
DR EMBL; CP002688; AED94834.1; -; Genomic_DNA.
DR EMBL; BT020312; AAV85667.1; -; mRNA.
DR EMBL; BT020456; AAW30034.1; -; mRNA.
DR RefSeq; NP_199074.1; NM_123624.4.
DR AlphaFoldDB; Q9FJV8; -.
DR SMR; Q9FJV8; -.
DR STRING; 3702.AT5G42600.1; -.
DR PaxDb; Q9FJV8; -.
DR PRIDE; Q9FJV8; -.
DR ProteomicsDB; 236722; -.
DR EnsemblPlants; AT5G42600.1; AT5G42600.1; AT5G42600.
DR GeneID; 834267; -.
DR Gramene; AT5G42600.1; AT5G42600.1; AT5G42600.
DR KEGG; ath:AT5G42600; -.
DR Araport; AT5G42600; -.
DR TAIR; locus:2163837; AT5G42600.
DR eggNOG; KOG0497; Eukaryota.
DR HOGENOM; CLU_009074_2_0_1; -.
DR InParanoid; Q9FJV8; -.
DR OrthoDB; 365003at2759; -.
DR PhylomeDB; Q9FJV8; -.
DR BioCyc; MetaCyc:AT5G42600-MON; -.
DR BRENDA; 5.4.99.53; 399.
DR PRO; PR:Q9FJV8; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FJV8; baseline and differential.
DR Genevisible; Q9FJV8; AT.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:TAIR.
DR GO; GO:0005811; C:lipid droplet; IEA:InterPro.
DR GO; GO:0042300; F:beta-amyrin synthase activity; IBA:GO_Central.
DR GO; GO:0034074; F:marneral synthase activity; IDA:TAIR.
DR GO; GO:0016104; P:triterpenoid biosynthetic process; IDA:TAIR.
DR CDD; cd02892; SQCY_1; 1.
DR InterPro; IPR032696; SQ_cyclase_C.
DR InterPro; IPR032697; SQ_cyclase_N.
DR InterPro; IPR018333; Squalene_cyclase.
DR InterPro; IPR002365; Terpene_synthase_CS.
DR InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR PANTHER; PTHR11764; PTHR11764; 1.
DR Pfam; PF13243; SQHop_cyclase_C; 1.
DR Pfam; PF13249; SQHop_cyclase_N; 1.
DR SFLD; SFLDG01016; Prenyltransferase_Like_2; 1.
DR SUPFAM; SSF48239; SSF48239; 2.
DR TIGRFAMs; TIGR01787; squalene_cyclas; 1.
DR PROSITE; PS01074; TERPENE_SYNTHASES; 1.
PE 1: Evidence at protein level;
KW Isomerase; Reference proteome; Repeat.
FT CHAIN 1..761
FT /note="Marneral synthase"
FT /id="PRO_0000366140"
FT REPEAT 149..190
FT /note="PFTB 1"
FT REPEAT 516..568
FT /note="PFTB 2"
FT REPEAT 642..683
FT /note="PFTB 3"
FT ACT_SITE 487
FT /note="Proton donor"
FT /evidence="ECO:0000250|UniProtKB:P48449"
SQ SEQUENCE 761 AA; 87207 MW; B92CB9DE5791E195 CRC64;
MWRLRIGAEA RQDPHLFTTN NFAGRQIWEF DANGGSPEEL AEVEEARLNF ANNKSRFKAS
PDLFWRRQFL REKKFEQKIP RVRIEDAEKI TYEDAKTALR RGVLYYAACQ ANDGHWPSEV
SGSMFLDAPF VICLYITGHL EKIFTLEHVK ELLRYMYNTQ NEDGGWGLDV ESHSVMFCTV
LNYICLRILG VEPDHDGQKS ACARARKWIL DHGGATYAPM VAKAWLSVLG VYDWSGCKPL
PPEIWMLPSF SPINGGTLWI YIRDLLMGMS YLYGKKFVAT PTALILQLRE ELYPQPYSKI
IWSKARNRCA KEDLLYPKSF GQDLFWEGVH MLSENIINRW PLNKFVRQRA LRTTMELVHY
HDETTHYITG ACVAKPFHML ACWVEDPDGD YFKKHLARVP DFIWIAEDGL KFQLMGMQSW
NAALSLQVML AANMDDEIRS TLIKGYDFLK QSQISENPQG DHLKMFRDIT KGGWTFQDRE
QGLPISDGTA ESIECCIHFH RMPSEFIGEK MDVEKLYDAV NFLIYLQSDN GGMPVWEPAP
GKKWLEWLSP VEHVENTVVE QEYLECTGSV IAGLVCFKKE FPDHRPKEIE KLIKKGLKYI
EDLQMPDGSW YGNWGVCFTY GTLFAVRGLA AAGKTFGNSE AIRRAVQFIL NTQNAEGGWG
ESALSCPNKK YIPSKGNVTN VVNTGQAMMV LLIGGQMERD PSPVHRAAKV LINSQLDIGD
FPQQERRGIY MNMLLHYPTY RNMFSLWALA LYTNALRLLV S
