ID   PENKA_XENLA             Reviewed;         262 AA.
AC   P01212;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 2.
DT   03-AUG-2022, entry version 77.
DE   RecName: Full=Proenkephalin-A-A;
DE   Contains:
DE     RecName: Full=Synenkephalin;
DE   Contains:
DE     RecName: Full=Met-enkephalin;
DE   Contains:
DE     RecName: Full=Met-enkephalin-Arg-Gly-Tyr;
DE   Contains:
DE     RecName: Full=Met-enkephalin-Arg-Phe;
DE   Flags: Precursor;
GN   Name=penk-a;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1721992; DOI=10.1016/0169-328x(91)90028-v;
RA   Wong M., Rius R.A., Loh Y.P.;
RT   "Characterization of Xenopus laevis proenkephalin gene.";
RL   Brain Res. Mol. Brain Res. 11:197-205(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 47-262.
RX   PubMed=6547769; DOI=10.1038/310251a0;
RA   Martens G.J.M., Herbert E.;
RT   "Polymorphism and absence of Leu-enkephalin sequences in proenkephalin
RT   genes in Xenopus laevis.";
RL   Nature 310:251-254(1984).
CC   -!- FUNCTION: Enkephalin neuropeptides compete with and mimic the effects
CC       of opiate drugs. They play a role in a number of physiologic functions,
CC       including pain perception and responses to stress.
CC       {ECO:0000250|UniProtKB:P01210}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P01210}.
CC   -!- PTM: The N-terminal domain contains 6 conserved cysteines thought to be
CC       involved in disulfide bonding and/or processing.
CC       {ECO:0000250|UniProtKB:P01210}.
CC   -!- SIMILARITY: Belongs to the opioid neuropeptide precursor family.
CC       {ECO:0000305}.
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DR   EMBL; S72932; AAB20686.1; -; Genomic_DNA.
DR   EMBL; S72930; AAB20686.1; JOINED; Genomic_DNA.
DR   EMBL; X00852; CAA25405.1; -; Genomic_DNA.
DR   PIR; A43949; EQXL.
DR   AlphaFoldDB; P01212; -.
DR   Proteomes; UP000186698; Genome assembly.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0001515; F:opioid peptide activity; IEA:UniProtKB-KW.
DR   GO; GO:0007218; P:neuropeptide signaling pathway; IEA:UniProtKB-KW.
DR   InterPro; IPR006024; Opioid_neupept.
DR   InterPro; IPR000703; Proenkphlin_A.
DR   PANTHER; PTHR11438; PTHR11438; 1.
DR   PANTHER; PTHR11438:SF3; PTHR11438:SF3; 1.
DR   Pfam; PF01160; Opiods_neuropep; 1.
DR   PRINTS; PR01028; OPIOIDPRCRSR.
DR   PRINTS; PR01029; PENKAPRCRSR.
DR   PROSITE; PS01252; OPIOIDS_PRECURSOR; 1.
PE   3: Inferred from homology;
KW   Cleavage on pair of basic residues; Disulfide bond; Endorphin;
KW   Neuropeptide; Opioid peptide; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   PEPTIDE         25..93
FT                   /note="Synenkephalin"
FT                   /id="PRO_0000008294"
FT   PEPTIDE         96..100
FT                   /note="Met-enkephalin"
FT                   /id="PRO_0000008295"
FT   PEPTIDE         103..107
FT                   /note="Met-enkephalin"
FT                   /id="PRO_0000008296"
FT   PROPEP          110..131
FT                   /id="PRO_0000008297"
FT   PEPTIDE         132..136
FT                   /note="Met-enkephalin"
FT                   /id="PRO_0000008298"
FT   PROPEP          139..177
FT                   /id="PRO_0000008299"
FT   PEPTIDE         180..187
FT                   /note="Met-enkephalin-Arg-Gly-Tyr"
FT                   /id="PRO_0000008300"
FT   PROPEP          190..201
FT                   /id="PRO_0000008301"
FT   PEPTIDE         204..208
FT                   /note="Met-enkephalin"
FT                   /id="PRO_0000008302"
FT   PROPEP          211..221
FT                   /id="PRO_0000008303"
FT   PEPTIDE         224..228
FT                   /note="Met-enkephalin"
FT                   /id="PRO_0000008304"
FT   PROPEP          229..253
FT                   /id="PRO_0000008305"
FT   PEPTIDE         256..262
FT                   /note="Met-enkephalin-Arg-Phe"
FT                   /id="PRO_0000008306"
FT   DISULFID        26..48
FT                   /evidence="ECO:0000250|UniProtKB:P01210"
FT   DISULFID        30..52
FT                   /evidence="ECO:0000250|UniProtKB:P01210"
FT   DISULFID        33..65
FT                   /evidence="ECO:0000250|UniProtKB:P01210"
SQ   SEQUENCE   262 AA;  29932 MW;  5B51C6133218B072 CRC64;
     MGLEARHCCM FLLVFASLSV EIRADCSKDC ASCALHLGQQ REINSLACTL ECEGKLPSAK
     AWGTCKELLL LTKVDNVQDG EKYQDNNDSH YAAKKYGGFM KRYGGFMKKM DELYHAEPEE
     DDAGGEILAK NYGGFMKKEY DSDRDAADLL RELLATSGDP ESSIYHDNNS ETPGEINKRY
     GGFMRGYRRS TDLEDETSGI QKRYGGFMRR VGRPEWWEDY QKRYGGFMTR FTDSFLPSDE
     DGESYSKENP DMEKRYGGFM RF