PENKV_MEIAT
ID PENKV_MEIAT Reviewed; 238 AA.
AC P0DP56;
DT 07-JUN-2017, integrated into UniProtKB/Swiss-Prot.
DT 07-JUN-2017, sequence version 1.
DT 25-MAY-2022, entry version 11.
DE RecName: Full=Proenkephalin-A;
DE AltName: Full=Proenkephalin {ECO:0000303|PubMed:28366739};
DE Contains:
DE RecName: Full=Synenkephalin {ECO:0000305};
DE Contains:
DE RecName: Full=Met-enkephalin {ECO:0000305};
DE AltName: Full=Opioid growth factor;
DE Short=OGF;
DE Contains:
DE RecName: Full=Met-enkephalin-His-Asp {ECO:0000305};
DE Contains:
DE RecName: Full=Met-enkephalin-Ser {ECO:0000305};
DE Flags: Precursor;
OS Meiacanthus atrodorsalis (Forktail blenny) (Petroscirtes atrodorsalis).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Ovalentaria; Blenniimorphae; Blenniiformes; Blennioidei; Blenniidae;
OC Blenniinae; Meiacanthus.
OX NCBI_TaxID=1405650;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX PubMed=28366739; DOI=10.1016/j.cub.2017.02.067;
RA Casewell N.R., Visser J.C., Baumann K., Dobson J., Han H., Kuruppu S.,
RA Morgan M., Romilio A., Weisbecker V., Ali S.A., Debono J., Koludarov I.,
RA Que I., Bird G.C., Cooke G.M., Nouwens A., Hodgson W.C., Wagstaff S.C.,
RA Cheney K.L., Vetter I., van der Weerd L., Richardson M.K., Fry B.G.;
RT "The evolution of fangs, venom, and mimicry systems in blenny fishes.";
RL Curr. Biol. 27:1184-1191(2017).
CC -!- FUNCTION: Met-enkephalins compete with and mimic the effects of opiate
CC drugs. They play a role in a number of physiologic functions, including
CC pain perception and responses to stress. Enkephalin peptides found in
CC Meiacanthus fangblennies induce physiological effects via their
CC interaction with delta-type opioid receptors (OPRD1) (tested on
CC M.grammistes). Therefore, finding a proenkephalin sequence in
CC M.atrodorsalis venom suggests that this protein act in the same manner
CC (Probable). {ECO:0000305|PubMed:28366739}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:28366739}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland. Moderately expressed
CC in the venom gland transcriptome. {ECO:0000269|PubMed:28366739}.
CC -!- MISCELLANEOUS: In contrast to venom of most venomous species, the venom
CC of Meiacanthus fangblennies is relatively painless, when tested on
CC mammals. While species-specific nociceptive effects are possible, this
CC defensive venom is surprisingly multifunctional, being markedly
CC hypotensive (via neuropeptide Y and/ or enkephalins), weakly neurotoxic
CC (unknown components, possibly PLA2s), and perhaps also pro-inflammatory
CC (PLA2s and/or enkephalins). The pronounced hypotensive effects induced
CC by venom peptides seem highly likely to affect the coordination and/or
CC swim performance of envenomed fishes and therefore likely confer a
CC fitness advantage to the fangblenny by facilitating escape from
CC predators. {ECO:0000269|PubMed:28366739}.
CC -!- SIMILARITY: Belongs to the opioid neuropeptide precursor family.
CC {ECO:0000305}.
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DR AlphaFoldDB; P0DP56; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0001515; F:opioid peptide activity; IEA:UniProtKB-KW.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR GO; GO:0007218; P:neuropeptide signaling pathway; IEA:UniProtKB-KW.
DR InterPro; IPR006024; Opioid_neupept.
DR InterPro; IPR000703; Proenkphlin_A.
DR PANTHER; PTHR11438; PTHR11438; 1.
DR PANTHER; PTHR11438:SF3; PTHR11438:SF3; 1.
DR Pfam; PF01160; Opiods_neuropep; 1.
DR PRINTS; PR01028; OPIOIDPRCRSR.
PE 2: Evidence at transcript level;
KW Cleavage on pair of basic residues; Disulfide bond; Endorphin;
KW G-protein coupled receptor impairing toxin; Neuropeptide; Opioid peptide;
KW Secreted; Signal; Toxin.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT PEPTIDE 26..106
FT /note="Synenkephalin"
FT /id="PRO_0000440267"
FT PEPTIDE 109..113
FT /note="Met-enkephalin"
FT /id="PRO_0000440268"
FT PEPTIDE 116..121
FT /note="Met-enkephalin-Ser"
FT /evidence="ECO:0000305"
FT /id="PRO_0000440269"
FT PROPEP 124..167
FT /evidence="ECO:0000305"
FT /id="PRO_0000440270"
FT PEPTIDE 170..174
FT /note="Met-enkephalin"
FT /id="PRO_0000440271"
FT PROPEP 177..195
FT /evidence="ECO:0000305"
FT /id="PRO_0000440272"
FT PEPTIDE 199..203
FT /note="Met-enkephalin"
FT /id="PRO_0000440273"
FT PROPEP 206..230
FT /evidence="ECO:0000305"
FT /id="PRO_0000440274"
FT PEPTIDE 233..238
FT /note="Met-enkephalin-His-Asp"
FT /evidence="ECO:0000305"
FT /id="PRO_0000440275"
FT REGION 76..103
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 88..103
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT DISULFID 27..50
FT /evidence="ECO:0000250|UniProtKB:P01210"
FT DISULFID 31..54
FT /evidence="ECO:0000250|UniProtKB:P01210"
FT DISULFID 34..66
FT /evidence="ECO:0000250|UniProtKB:P01210"
SQ SEQUENCE 238 AA; 26014 MW; 039E4388E6E790C4 CRC64;
MAASALSTCL WMLVLGTCVS LVVGTDCGKE CALCVYRLLG QQSTLSSLTC SLECDGGLDS
QKLRLCQDVL LEEENQSPLA SQQDQERVDA MMADEEDATS PEHQMAKKYG GFMKRYGGFM
SRRSSASSLE EAGNQDEEQS IRTEILKILN AATEHGGEGD GQEAEAVKRY GGFMRRADRG
AAQGNLVEAV LGRVLKKRYG GFMRRVGRPE WLVDSSKKVG VLKENGSELQ KRHGGFMD
