PENV_PENRW
ID PENV_PENRW Reviewed; 832 AA.
AC B6HTR9;
DT 25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT 16-DEC-2008, sequence version 1.
DT 03-AUG-2022, entry version 65.
DE RecName: Full=Vacuolar transmembrane transporter penV {ECO:0000303|PubMed:22777282};
GN Name=penV {ECO:0000303|PubMed:22777282}; ORFNames=Pc22g22150;
OS Penicillium rubens (strain ATCC 28089 / DSM 1075 / NRRL 1951 / Wisconsin
OS 54-1255) (Penicillium chrysogenum).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Penicillium;
OC Penicillium chrysogenum species complex.
OX NCBI_TaxID=500485;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 28089 / DSM 1075 / NRRL 1951 / Wisconsin 54-1255;
RX PubMed=18820685; DOI=10.1038/nbt.1498;
RA van den Berg M.A., Albang R., Albermann K., Badger J.H., Daran J.-M.,
RA Driessen A.J.M., Garcia-Estrada C., Fedorova N.D., Harris D.M.,
RA Heijne W.H.M., Joardar V.S., Kiel J.A.K.W., Kovalchuk A., Martin J.F.,
RA Nierman W.C., Nijland J.G., Pronk J.T., Roubos J.A., van der Klei I.J.,
RA van Peij N.N.M.E., Veenhuis M., von Doehren H., Wagner C., Wortman J.R.,
RA Bovenberg R.A.L.;
RT "Genome sequencing and analysis of the filamentous fungus Penicillium
RT chrysogenum.";
RL Nat. Biotechnol. 26:1161-1168(2008).
RN [2]
RP FUNCTION, DISRUPTION PHENOTYPE, AND SUBCELLULAR LOCATION.
RX PubMed=22777282; DOI=10.1007/s00253-012-4256-0;
RA Fernandez-Aguado M., Teijeira F., Martin J.F., Ullan R.V.;
RT "A vacuolar membrane protein affects drastically the biosynthesis of the
RT ACV tripeptide and the beta-lactam pathway of Penicillium chrysogenum.";
RL Appl. Microbiol. Biotechnol. 97:795-808(2013).
CC -!- FUNCTION: Vacuolar transmembrane transporter that participates in the
CC first stage of the beta-lactam biosynthesis (the formation of the ACV
CC tripeptide), probably taking part in the supply of amino acids from the
CC vacuolar lumen to the vacuole-anchored ACV synthetase.
CC {ECO:0000269|PubMed:22777282}.
CC -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000269|PubMed:22777282};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- DISRUPTION PHENOTYPE: Leads to a drastic reduction in the production of
CC penicillin and its biosynthetic intermediates delta-(L-alpha-
CC aminoadipyl-L-cysteinyl-D-valine) and isopenicillin N.
CC {ECO:0000269|PubMed:22777282}.
CC -!- SIMILARITY: Belongs to the CSC1 (TC 1.A.17) family. {ECO:0000305}.
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DR EMBL; AM920437; CAP99503.1; -; Genomic_DNA.
DR RefSeq; XP_002566110.1; XM_002566064.1.
DR STRING; 1108849.XP_002566110.1; -.
DR EnsemblFungi; CAP99503; CAP99503; PCH_Pc22g22150.
DR GeneID; 8308377; -.
DR KEGG; pcs:Pc22g22150; -.
DR VEuPathDB; FungiDB:PCH_Pc22g22150; -.
DR eggNOG; KOG1134; Eukaryota.
DR HOGENOM; CLU_002458_0_0_1; -.
DR OMA; QGIGMFP; -.
DR OrthoDB; 395194at2759; -.
DR BioCyc; PCHR:PC22G22150-MON; -.
DR Proteomes; UP000000724; Contig Pc00c22.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005227; F:calcium activated cation channel activity; IEA:InterPro.
DR GO; GO:0006865; P:amino acid transport; IEA:UniProtKB-KW.
DR InterPro; IPR045122; Csc1-like.
DR InterPro; IPR032880; Csc1_N.
DR InterPro; IPR027815; PHM7_cyt.
DR InterPro; IPR003864; RSN1_7TM.
DR PANTHER; PTHR13018; PTHR13018; 1.
DR Pfam; PF14703; PHM7_cyt; 1.
DR Pfam; PF02714; RSN1_7TM; 1.
DR Pfam; PF13967; RSN1_TM; 1.
PE 3: Inferred from homology;
KW Amino-acid transport; Glycoprotein; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport; Vacuole.
FT CHAIN 1..832
FT /note="Vacuolar transmembrane transporter penV"
FT /id="PRO_0000455150"
FT TRANSMEM 39..59
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 117..137
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 178..198
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 434..454
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 483..503
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 524..544
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 560..582
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 587..608
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 623..645
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 650..672
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 687..707
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 713..733
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 152..171
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 291..322
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 754..777
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 302..322
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 755..772
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 158
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 214
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 832 AA; 94489 MW; 26352879203881BE CRC64;
MSLTASAASA ALTLLADGPD DGSKRPWRPN DPVKEQRDLY TQFVISTALG LSAFLAFCIL
RPKWTELYAA RRRQRNAASR LPELPDTLFG WIPVLHQITE EEVLQSAGLD AYVFLSFFKF
AIRFLLAVFI FAVAIILPMH YKYTGQYGVP GWDNPPGNKT TSPIDGSEKE KPVTDPAYLW
IYVLFAYVFS GLAIYMLLDE TKVIIRTRQT YLGNQTSTTD RTIRLSGIPH DLGTEDKIKE
FVEGLRVGKV ESITVCRKWR ELDELIDERM KVIRELERAW TKHIGYKRPK NDGNALPLTE
QQPRDADDER SGLLSGHDNE HVSGYSNERP KVRIWYGLFK LRFRMIDAID YYEEKLRKID
EYIQNAREKE YRTTEIAFVT MESIAASQML VQAILDPHPM QMFARLAPAP ADVIWKNTYL
SRTRRMVQSW SITFVIGFLT VFWSVLLVPI ASLLELKTLE TIVPRLAEFL QEHPIIKSLV
QTGLPTLAFS LLTVAVPYLY EWLSNNQGMV SRGDVELSVI SKNFFFSFFN LFLLFTVFGT
ASGFYGFWES LRDAFKDSTT IALALANSLE GLAPFYINLL ILQGLGLFPF RLLEFGSVAL
YPFQFLSART PREYAELSTP PKFSYGFSIP QTILILVICV VYSVFPSSWL ICLFGLIYFT
VGKFIYKYQL LYAMDHQQHS TGRAWPMICN RVFVGLLVHQ LAMIGVLALR RAITRSLLLV
PLLGFTVWFS YWFGRTYEPL MKFIALKSIN RDQPGGGDIS PSPSSTLSPP SGLDRDSLPI
RIGGQDLELR LKKYVNPSLI VPLHAAWLPG RNPARGNGNG ASAFEVHQTQ NV
