PEP8_YEAST
ID PEP8_YEAST Reviewed; 379 AA.
AC P40335; D6VWC9; E9P910;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 2.
DT 03-AUG-2022, entry version 180.
DE RecName: Full=Carboxypeptidase Y-deficient protein 8;
DE AltName: Full=Vacuolar protein sorting-associated protein 26;
DE AltName: Full=Vacuolar protein-targeting protein 4;
GN Name=PEP8; Synonyms=GRD6, VPS26, VPT4; OrderedLocusNames=YJL053W;
GN ORFNames=J1152;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 26109 / X2180;
RX PubMed=7926738; DOI=10.1101/gad.8.12.1379;
RA Bachhawat A.K., Suhan J., Jones E.W.;
RT "The yeast homolog of H58, a mouse gene essential for embryogenesis,
RT performs a role in the delivery of proteins to the vacuole.";
RL Genes Dev. 8:1379-1387(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8641269; DOI=10.1002/j.1460-2075.1996.tb00557.x;
RA Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C.,
RA Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D.,
RA Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J.,
RA Heumann K., Hilger F., Hollenberg C.P., Huang M.-E., Jacq C.,
RA Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E.,
RA Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T.,
RA Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R.,
RA Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N.,
RA To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H.,
RA von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.";
RL EMBO J. 15:2031-2049(1996).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP IDENTIFICATION IN THE RETROMER COMPLEX.
RX PubMed=9700157; DOI=10.1083/jcb.142.3.665;
RA Seaman M.N., McCaffery J.M., Emr S.D.;
RT "A membrane coat complex essential for endosome-to-Golgi retrograde
RT transport in yeast.";
RL J. Cell Biol. 142:665-681(1998).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-216, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- FUNCTION: Plays a role in vesicular protein sorting. Required for the
CC endosome-to-Golgi retrieval of the vacuolar protein sorting receptor
CC VPS10. Component of the membrane-associated retromer complex which is
CC essential in endosome-to-Golgi retrograde transport. The VPS29-VPS26-
CC VPS35 subcomplex may be involved in cargo selection.
CC -!- SUBUNIT: Component of the retromer complex which consists of VPS29,
CC VPS26, VPS35, VPS5 and VPS17. Component of a retromer subcomplex
CC consisting of VPS29, VPS26 and VPS35. {ECO:0000269|PubMed:9700157}.
CC -!- SIMILARITY: Belongs to the VPS26 family. {ECO:0000305}.
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DR EMBL; L11623; AAA34853.1; -; Genomic_DNA.
DR EMBL; Z49328; CAA89344.1; -; Genomic_DNA.
DR EMBL; AY693007; AAT93026.1; -; Genomic_DNA.
DR EMBL; BK006943; DAA08745.1; -; Genomic_DNA.
DR PIR; S56825; S56825.
DR RefSeq; NP_012482.1; NM_001181486.1.
DR AlphaFoldDB; P40335; -.
DR SMR; P40335; -.
DR BioGRID; 33701; 369.
DR ComplexPortal; CPX-1653; Retromer complex.
DR DIP; DIP-4572N; -.
DR IntAct; P40335; 12.
DR MINT; P40335; -.
DR STRING; 4932.YJL053W; -.
DR TCDB; 9.A.63.1.1; the retromer-dependent vacuolar protein sorting (r-vps) family.
DR iPTMnet; P40335; -.
DR MaxQB; P40335; -.
DR PaxDb; P40335; -.
DR PRIDE; P40335; -.
DR EnsemblFungi; YJL053W_mRNA; YJL053W; YJL053W.
DR GeneID; 853393; -.
DR KEGG; sce:YJL053W; -.
DR SGD; S000003589; PEP8.
DR VEuPathDB; FungiDB:YJL053W; -.
DR eggNOG; KOG3063; Eukaryota.
DR GeneTree; ENSGT00950000183064; -.
DR HOGENOM; CLU_031077_2_0_1; -.
DR InParanoid; P40335; -.
DR OMA; LWVHSYR; -.
DR BioCyc; YEAST:G3O-31516-MON; -.
DR Reactome; R-SCE-3238698; WNT ligand biogenesis and trafficking.
DR PRO; PR:P40335; -.
DR Proteomes; UP000002311; Chromosome X.
DR RNAct; P40335; protein.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0005829; C:cytosol; IEA:GOC.
DR GO; GO:0005768; C:endosome; IDA:SGD.
DR GO; GO:0000329; C:fungal-type vacuole membrane; HDA:SGD.
DR GO; GO:0030904; C:retromer complex; IMP:SGD.
DR GO; GO:0030906; C:retromer, cargo-selective complex; IMP:SGD.
DR GO; GO:0140318; F:protein transporter activity; IMP:SGD.
DR GO; GO:0006886; P:intracellular protein transport; IBA:GO_Central.
DR GO; GO:0045053; P:protein retention in Golgi apparatus; IMP:SGD.
DR GO; GO:0042147; P:retrograde transport, endosome to Golgi; IPI:SGD.
DR Gene3D; 2.60.40.640; -; 2.
DR InterPro; IPR014752; Arrestin-like_C.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR028934; Vps26-related.
DR PANTHER; PTHR12233; PTHR12233; 1.
DR Pfam; PF03643; Vps26; 1.
DR SUPFAM; SSF81296; SSF81296; 1.
PE 1: Evidence at protein level;
KW Phosphoprotein; Protein transport; Reference proteome; Transport.
FT CHAIN 1..379
FT /note="Carboxypeptidase Y-deficient protein 8"
FT /id="PRO_0000073015"
FT REGION 84..108
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 216
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT CONFLICT 189
FT /note="A -> E (in Ref. 1; AAA34853)"
FT /evidence="ECO:0000305"
FT CONFLICT 286
FT /note="R -> K (in Ref. 4; AAT93026)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 379 AA; 42547 MW; 40D15594E70A9B58 CRC64;
MSIFFKPPID IEILFDNEES RKHVDIATRS SNSSYKSMKE SLPVYEDGES LGGIVTLRVR
DSKKVDHLGI KVSVIGSIDM LKSHGSGNSS SKKVTSSTSS SSSNGSVDVR KNSVDQFLCQ
SYDLCPAGEL QHSQSFPFLF RDLSKRYESY KGKNVDVAYY VKVTVMRKST DISKIKRFWV
YLYNSVTTAP NTLSANETKA TTNDIAGGNY AADNASDNTQ TKSTQGEAAD VNQVLPISHS
NNEPKPVRLD IGIENCLHIE FEYAKSQYSL KEVIVGRIYF LLTRLRIKHM ELSLITRESS
GLQTSNVMTD STAIRYEIMD GSSVKGETIP IRLFLSGYDL TPNMSCNYFN VKNYLSLVII
DEDGRRYFKQ SEITLYRTR
