ID   PEPCK_PROFF             Reviewed;        1131 AA.
AC   A0A0C5URS1;
DT   11-NOV-2015, integrated into UniProtKB/Swiss-Prot.
DT   29-APR-2015, sequence version 1.
DT   25-MAY-2022, entry version 19.
DE   RecName: Full=PPi-type phosphoenolpyruvate carboxykinase {ECO:0000303|PubMed:26269598};
DE            Short=PPi-PEPCK {ECO:0000303|PubMed:26269598};
DE            Short=PfPEPCK {ECO:0000303|PubMed:26269598};
DE            EC=4.1.1.38 {ECO:0000269|PubMed:26269598};
DE   AltName: Full=Diphosphate-dependent phosphoenolpyruvate carboxykinase {ECO:0000305};
DE   AltName: Full=PEP carboxyphosphotransferase {ECO:0000305};
GN   ORFNames=RM25_0213 {ECO:0000312|EMBL:AJQ89945.1};
OS   Propionibacterium freudenreichii subsp. freudenreichii.
OC   Bacteria; Actinobacteria; Propionibacteriales; Propionibacteriaceae;
OC   Propionibacterium.
OX   NCBI_TaxID=66712;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 6207 / DSM 20271 / LMG 16412 / NBRC 12424 / NCIMB 5959 / NCTC
RC   10470 / NRRL B-3523 {ECO:0000312|EMBL:AJQ89945.1};
RA   Koskinen P., Deptula P., Smolander O.-P., Kammonen J., Savijoki K.,
RA   Paulin L., Piironen V., Auvinen P., Varmanen P.;
RT   "Complete genome sequence of Propionibacterium freudenreichii DSM 20271T.";
RL   Submitted (DEC-2014) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION, AND CATALYTIC ACTIVITY.
RX   PubMed=26269598; DOI=10.1074/jbc.m115.672907;
RA   Chiba Y., Kamikawa R., Nakada-Tsukui K., Saito-Nakano Y., Nozaki T.;
RT   "Discovery of PPi-type phosphoenolpyruvate carboxykinase genes in
RT   eukaryotes and bacteria.";
RL   J. Biol. Chem. 290:23960-23970(2015).
CC   -!- FUNCTION: Inorganic pyrophosphate (PPi)-dependent phosphoenolpyruvate
CC       carboxykinase, which regulates the carbon flow of the central
CC       metabolism by fixing CO(2) to phosphoenolpyruvate to produce
CC       oxaloacetate. Can also produce pyruvate and diphosphate from
CC       phosphoenolpyruvate and phosphate. {ECO:0000269|PubMed:26269598}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=diphosphate + oxaloacetate = CO2 + phosphate +
CC         phosphoenolpyruvate; Xref=Rhea:RHEA:22356, ChEBI:CHEBI:16452,
CC         ChEBI:CHEBI:16526, ChEBI:CHEBI:33019, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:58702; EC=4.1.1.38;
CC         Evidence={ECO:0000269|PubMed:26269598};
CC   -!- SUBUNIT: Monomer and trimer; forms heterotrimers with PEPCK2 and
CC       PEPCK3. {ECO:0000269|PubMed:26269598}.
CC   -!- SIMILARITY: Belongs to the PPi-type phosphoenolpyruvate carboxykinase
CC       family. {ECO:0000305}.
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DR   EMBL; CP010341; AJQ89945.1; -; Genomic_DNA.
DR   RefSeq; WP_044635901.1; NZ_CP010341.1.
DR   AlphaFoldDB; A0A0C5URS1; -.
DR   SMR; A0A0C5URS1; -.
DR   KEGG; pfre:RM25_0213; -.
DR   PATRIC; fig|66712.6.peg.219; -.
DR   HOGENOM; CLU_275663_0_0_11; -.
DR   GO; GO:0030585; F:phosphoenolpyruvate carboxykinase (diphosphate) activity; IDA:UniProtKB.
PE   1: Evidence at protein level;
KW   Decarboxylase; Lyase.
FT   CHAIN           1..1131
FT                   /note="PPi-type phosphoenolpyruvate carboxykinase"
FT                   /id="PRO_0000434733"
SQ   SEQUENCE   1131 AA;  127040 MW;  C13E3EE1A465BBC9 CRC64;
     MSVVERRQIN AAINLRLSLL GLPHPDSNAE SPDAILVEPL LARQRELSRR LKDRLSAPDL
     RIQRFLDDYL ADCDEHPQLP RTTLVLDEPG LARGLSLPVD GDEFHSDIVA SYRLVNGVLH
     NPKHDRRTTA GVFHISTGGL PIPQDKVEVD KNVYARILAR AFQAPDEELA LPYTANLPEQ
     AHCWASLLMR PTVLPAVPGR TTEKSYEVHF IVPGGLMCNL DFVEGIFGNA GDPYLPENDA
     SLDPDSWTGH TGCVILAPHL TTMTKKSLGM PHYDDATERQ RRDGQCWRHE DDLYNDGKAF
     KVCARDERGV IVTVIADNYF GYCKKEVKTQ ISYSANLLGG AEEEHSGGAE VYPAWNLNQD
     FTDRTPDDFT LADVISTNRE LLDVRPEGYA VYKPEPNIVF IPEHSHYSMR TQTISWTAHG
     AEQTIKLLAG KHYLSPDGYR IHAKHREMDA TQWHLIGTSS RAVTCHKPAT VSGGGKSEIS
     KSISDAFVFG NAFSHDIDSA MDQVQALFDT DFTNRFADAS RNGTDHRPVL SIDRSLGSVI
     KLLTPSIQYN DEYNAFLEGI EPDVKELAFT VKRYYLPEWG EDWRSHFTVG IMNGRHGNMV
     RLDGKKIITN MLRVGFREDG SWRLFTLRPD YSPAVKVQTE DDITASTVTP PWEDAEGLPR
     KYVTNCEHLL FQRPDDAIHR GYDKQAEFDL ASGTDTFISN FEPLTHEQAR DLLTDVQAYS
     EFTKPVRKLI ERVAAMPDDQ SPEFWVCSDD PRHLPDGGRS KNPRYLQVRP TDSNPELTTV
     ADVAGKLARK LPLAGHAPQP IDVVAAGRRN NPPEDKVPAL CAYNPLHYME LPELFMEYIS
     SMTGKSPSTT GAGSEGALTK GPFNALPAVY DLNAALLSYA LTDYDGWLSS AGYIGPNARV
     DHDISMLIPE LFSHMGPNDR NTKRLISEGY LEKMQDFDFD GHRVLASRLG YRINDRFVTH
     YFGRIFLHPD VVFSEEMLRP ELQDEKIFAD SIDVIVKTHQ RVAQMYFDDG TVSLACPPIR
     ALLEIMAHGA SAEGWTLDSP EFRKLFERES VLASDWYAQR LDAKQAEDVK QAEEGVERLK
     EYIGRSDSGS VTGRLHLADR LRELEAQLTY ERSPEYRQSL VGTLGRQPRF V