ASSY_DICCH
ID ASSY_DICCH Reviewed; 242 AA.
AC P0C1A0; P42181; Q9KHB9;
DT 18-APR-2006, integrated into UniProtKB/Swiss-Prot.
DT 18-APR-2006, sequence version 1.
DT 25-MAY-2022, entry version 46.
DE RecName: Full=Argininosuccinate synthase;
DE EC=6.3.4.5;
DE AltName: Full=Citrulline--aspartate ligase;
DE Flags: Fragment;
GN Name=argG;
OS Dickeya chrysanthemi (Pectobacterium chrysanthemi) (Erwinia chrysanthemi).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Pectobacteriaceae; Dickeya.
OX NCBI_TaxID=556;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=RA3B;
RA Huang H.-C., Chu M.-K., Hsu S.-T., Tzeng K.-C., Lin R.-H.;
RT "Molecular cloning of genes involved in blue pigment synthesis from Erwinia
RT chrysanthemi RA3B.";
RL Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-aspartate + L-citrulline = 2-(N(omega)-L-
CC arginino)succinate + AMP + diphosphate + H(+); Xref=Rhea:RHEA:10932,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:29991, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:57472, ChEBI:CHEBI:57743,
CC ChEBI:CHEBI:456215; EC=6.3.4.5;
CC -!- PATHWAY: Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine
CC from L-ornithine and carbamoyl phosphate: step 2/3.
CC -!- SUBUNIT: Homotetramer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the argininosuccinate synthase family. Type 2
CC subfamily. {ECO:0000305}.
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DR EMBL; AF265211; AAF74776.1; -; Genomic_DNA.
DR AlphaFoldDB; P0C1A0; -.
DR SMR; P0C1A0; -.
DR UniPathway; UPA00068; UER00113.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004055; F:argininosuccinate synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0042803; F:protein homodimerization activity; IEA:InterPro.
DR GO; GO:0006526; P:arginine biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 1.10.287.400; -; 1.
DR Gene3D; 3.90.1260.10; -; 1.
DR InterPro; IPR001518; Arginosuc_synth.
DR InterPro; IPR024074; AS_cat/multimer_dom_body.
DR InterPro; IPR024073; AS_multimer_C_tail.
DR PANTHER; PTHR11587; PTHR11587; 1.
DR Pfam; PF00764; Arginosuc_synth; 1.
DR SUPFAM; SSF69864; SSF69864; 1.
PE 3: Inferred from homology;
KW Amino-acid biosynthesis; Arginine biosynthesis; ATP-binding; Cytoplasm;
KW Ligase; Nucleotide-binding.
FT CHAIN <1..242
FT /note="Argininosuccinate synthase"
FT /id="PRO_0000148699"
FT NON_TER 1
SQ SEQUENCE 242 AA; 27349 MW; BF3C91A7FD7D49D5 CRC64;
EFLNSSVKIV NPIMGVKFWD ENVRIPAEEV TVRFERGHPV ALNGQTFSDD VELLLEANRI
GGRHGLGMSD QIENRIIEAK SRGIYEAPGM ALLHIAYERL VTGIHNEDTI EQYHAHGRQL
GRLLYQGRWF DPQALMLRDA LQRWVASEIT GEVTLELRRG NDYSILNTVS DNLTYKPERL
TMEKGESVFS PDDRIGQLTM RNLDITDTRE KLFNYVESGL ISSGNAGLPQ VANPLLQDKS
AK