ID   PEPF1_LACLC             Reviewed;         601 AA.
AC   P54124; P94880;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=Oligoendopeptidase F, plasmid;
DE            EC=3.4.24.-;
GN   Name=pepF1; Synonyms=pepF;
OS   Lactococcus lactis subsp. cremoris (Streptococcus cremoris).
OG   Plasmid pLP763.
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Lactococcus.
OX   NCBI_TaxID=1359;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
RC   STRAIN=NCDO 763 / ML3;
RX   PubMed=7798200; DOI=10.1016/s0021-9258(18)31602-8;
RA   Monnet V., Nardi M., Chopin A., Chopin M.-C., Gripon J.-C.;
RT   "Biochemical and genetic characterization of PepF, an oligopeptidase from
RT   Lactococcus lactis.";
RL   J. Biol. Chem. 269:32070-32076(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=NCDO 763 / ML3;
RX   PubMed=9209029; DOI=10.1128/jb.179.13.4164-4171.1997;
RA   Nardi M., Renault P., Monnet V.;
RT   "Duplication of the pepF gene and shuffling of DNA fragments on the lactose
RT   plasmid of Lactococcus lactis.";
RL   J. Bacteriol. 179:4164-4171(1997).
CC   -!- FUNCTION: Hydrolyzes peptides containing between 7 and 17 amino acids
CC       with a rather wide specificity.
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250};
CC       Note=Binds 1 zinc ion. {ECO:0000250};
CC   -!- SIMILARITY: Belongs to the peptidase M3B family. {ECO:0000305}.
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DR   EMBL; Z32522; CAA83534.1; -; Genomic_DNA.
DR   EMBL; X99798; CAA68133.1; -; Genomic_DNA.
DR   PIR; A55485; A55485.
DR   AlphaFoldDB; P54124; -.
DR   SMR; P54124; -.
DR   MEROPS; M03.007; -.
DR   BRENDA; 3.4.24.B2; 2903.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.1370.20; -; 1.
DR   InterPro; IPR013647; OligopepF_N_dom.
DR   InterPro; IPR042088; OligoPept_F_C.
DR   InterPro; IPR045090; Pept_M3A_M3B.
DR   InterPro; IPR001567; Pept_M3A_M3B_dom.
DR   InterPro; IPR004438; Peptidase_M3B.
DR   PANTHER; PTHR11804; PTHR11804; 1.
DR   Pfam; PF01432; Peptidase_M3; 1.
DR   Pfam; PF08439; Peptidase_M3_N; 1.
DR   TIGRFAMs; TIGR00181; pepF; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Hydrolase; Metal-binding; Metalloprotease;
KW   Plasmid; Protease; Zinc.
FT   CHAIN           1..601
FT                   /note="Oligoendopeptidase F, plasmid"
FT                   /id="PRO_0000078163"
FT   ACT_SITE        388
FT                   /evidence="ECO:0000250"
FT   BINDING         387
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000250"
FT   BINDING         391
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000250"
FT   BINDING         394
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        518
FT                   /note="F -> S (in Ref. 2; CAA68133)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   601 AA;  69675 MW;  C8B5C519FFA2F787 CRC64;
     MAKNRNEIPE KLTWDLTTIY KTDKEWEAEL TRIKSELSLV EETDPGHLLD SAESLLTITE
     KMLSISQQVE KLYVYASMKN DQDTREAKYQ EYQSKATALY VKFGEVYAFY EPEFLKISKE
     VYNKWLGELQ KLKNYDHMFE RLFAKKAHIL SQKEEKLLAA AGEIFESPSE TFEIFDNADI
     KLPMVKNESD EMIQLTHGNY SSLMESKNRG VRKAAYKALY SNYEQYQHTY AKTLQTNVKV
     HNLNAQIRSY DSARQAALAN NFVPEKVYDV LMEAIHQHLP LLHRYIELRK KILGITDLKM
     YGIYTPLSNL GYKFNYEDGV KKAEEVLAIF GKEYKGKVKA AFEQRWIDVE ENIGKRSGAY
     SGGSYDTNAF MLLNWQETLD DLFTLVHETG HSMHSAFTRE NQPYVYGNYP IFLAEIASTT
     NENILTETLL KESKDDKERF ALLNHWLDSF RGTVFRQSQF AEFEQKIHEA DAAGEVLTSE
     YLNSLYGEIN EKYYNLAVKG NPEIQYEWAR IPHFYYNFYV FQYATGFAAA TFLAEKVVHG
     STEDRQKYLE YLKAGSSAYP LEVIAKAGVD MESTDYLDAA FELFENRLSE LEKLVEKGVH
     L