ID   PEPF_LACLA              Reviewed;         601 AA.
AC   Q9CEV7;
DT   26-SEP-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   03-AUG-2022, entry version 112.
DE   RecName: Full=Oligoendopeptidase F homolog;
DE            EC=3.4.24.-;
GN   Name=pepF; OrderedLocusNames=LL1727; ORFNames=L166370;
OS   Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Lactococcus.
OX   NCBI_TaxID=272623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=IL1403;
RX   PubMed=11337471; DOI=10.1101/gr.gr-1697r;
RA   Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J.,
RA   Ehrlich S.D., Sorokin A.;
RT   "The complete genome sequence of the lactic acid bacterium Lactococcus
RT   lactis ssp. lactis IL1403.";
RL   Genome Res. 11:731-753(2001).
CC   -!- FUNCTION: Hydrolyzes peptides containing between 7 and 17 amino acids
CC       with a rather wide specificity. {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250};
CC       Note=Binds 1 zinc ion. {ECO:0000250};
CC   -!- SIMILARITY: Belongs to the peptidase M3 family. {ECO:0000305}.
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DR   EMBL; AE005176; AAK05825.1; -; Genomic_DNA.
DR   PIR; G86840; G86840.
DR   RefSeq; NP_267883.1; NC_002662.1.
DR   RefSeq; WP_010906109.1; NC_002662.1.
DR   AlphaFoldDB; Q9CEV7; -.
DR   SMR; Q9CEV7; -.
DR   STRING; 272623.L166370; -.
DR   MEROPS; M03.007; -.
DR   PaxDb; Q9CEV7; -.
DR   EnsemblBacteria; AAK05825; AAK05825; L166370.
DR   KEGG; lla:L166370; -.
DR   PATRIC; fig|272623.7.peg.1852; -.
DR   eggNOG; COG1164; Bacteria.
DR   HOGENOM; CLU_021290_2_0_9; -.
DR   OMA; YRQTMFA; -.
DR   Proteomes; UP000002196; Chromosome.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.1370.20; -; 1.
DR   InterPro; IPR013647; OligopepF_N_dom.
DR   InterPro; IPR042088; OligoPept_F_C.
DR   InterPro; IPR045090; Pept_M3A_M3B.
DR   InterPro; IPR001567; Pept_M3A_M3B_dom.
DR   InterPro; IPR004438; Peptidase_M3B.
DR   PANTHER; PTHR11804; PTHR11804; 1.
DR   Pfam; PF01432; Peptidase_M3; 1.
DR   Pfam; PF08439; Peptidase_M3_N; 1.
DR   TIGRFAMs; TIGR00181; pepF; 1.
DR   PROSITE; PS00142; ZINC_PROTEASE; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Metal-binding; Metalloprotease; Protease; Reference proteome;
KW   Zinc.
FT   CHAIN           1..601
FT                   /note="Oligoendopeptidase F homolog"
FT                   /id="PRO_0000078162"
FT   ACT_SITE        388
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10095"
FT   BINDING         387
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10095"
FT   BINDING         391
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10095"
FT   BINDING         394
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10095"
SQ   SEQUENCE   601 AA;  69884 MW;  045E6873F10FE19A CRC64;
     MAKNRNEITE KLTWDLTTIY KTDKEWEAEL TRIKSELSLV EETDPGHLLD SAESLLTITE
     KMLSISQQVE KLYVYASMKN DQDTREAKYQ EYQSKATALY VKFGEVYAFY EPEFLKISKE
     VYNKWLGELQ KLKNYDHMFE RLFAKKAHIL SQKEEKLLAA AGEIFESPSE TFEIFDNADI
     KLPMVKNESD EMIQLTHGNY SSLMESKNRG VRKAAYKALY SNYEQYQHTY AKTLQTNVKV
     HNLKAQIRSY DSARQAALAN NFVPEKVYDV LMEAIHQHLP LLHRYIELRK KILGITDLKM
     YDIYTPLSNL DYKFNYEDGV KKAEEVLAIF GKEYKGKVKA AFEQRWIDVE ENIGKRSGAY
     SGGSYDTNAF MLLNWQETLD DLFTLVHEMG HSMHSAFTRE NQPYVYGDYP IFLAEIASTT
     NENILTETLL KESKDDKERF ALLNHWLDSF RGTVFRQSQF AEFEQKIHEA DAAGEVLTSE
     YLNSLYGEIN EKYYNLAAKE NPEIQYEWAR IPHFYYNFYV FQYATGFAAA TFLAEKVVHG
     STEDRQKYLE YLKAGSSAYP LEVIAKAGVD MESTDYLDAA FELFENRLSE LEKLVEKGVH
     L