PEPVL_STAAC
ID PEPVL_STAAC Reviewed; 469 AA.
AC Q5HF23; Q9KWZ7;
DT 03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 15-FEB-2005, sequence version 1.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=Putative dipeptidase SACOL1801;
DE EC=3.4.13.-;
GN OrderedLocusNames=SACOL1801;
OS Staphylococcus aureus (strain COL).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=93062;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=10566865; DOI=10.1089/mdr.1999.5.163;
RA de Lencastre H., Wu S.-W., Pinho M.G., Ludovice A.M., Filipe S.,
RA Gardete S., Sobral R., Gill S.R., Chung M., Tomasz A.;
RT "Antibiotic resistance as a stress response: complete sequencing of a large
RT number of chromosomal loci in Staphylococcus aureus strain COL that impact
RT on the expression of resistance to methicillin.";
RL Microb. Drug Resist. 5:163-175(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=COL;
RX PubMed=15774886; DOI=10.1128/jb.187.7.2426-2438.2005;
RA Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J.,
RA Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J.,
RA Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H.,
RA Vamathevan J.J., Khouri H., Utterback T.R., Lee C., Dimitrov G., Jiang L.,
RA Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E.,
RA Fraser C.M.;
RT "Insights on evolution of virulence and resistance from the complete genome
RT analysis of an early methicillin-resistant Staphylococcus aureus strain and
RT a biofilm-producing methicillin-resistant Staphylococcus epidermidis
RT strain.";
RL J. Bacteriol. 187:2426-2438(2005).
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250};
CC Note=Binds 2 Zn(2+) ions per subunit. {ECO:0000250};
CC -!- SIMILARITY: Belongs to the peptidase M20A family. {ECO:0000305}.
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DR EMBL; Y14816; CAB82474.1; -; Genomic_DNA.
DR EMBL; CP000046; AAW38329.1; -; Genomic_DNA.
DR RefSeq; WP_000265417.1; NC_002951.2.
DR PDB; 3KHX; X-ray; 2.30 A; A/B=1-469.
DR PDB; 3KHZ; X-ray; 2.50 A; A/B=1-469.
DR PDB; 3KI9; X-ray; 2.90 A; A=1-469.
DR PDBsum; 3KHX; -.
DR PDBsum; 3KHZ; -.
DR PDBsum; 3KI9; -.
DR AlphaFoldDB; Q5HF23; -.
DR SMR; Q5HF23; -.
DR EnsemblBacteria; AAW38329; AAW38329; SACOL1801.
DR KEGG; sac:SACOL1801; -.
DR HOGENOM; CLU_031786_2_0_9; -.
DR OMA; GYAGHIE; -.
DR EvolutionaryTrace; Q5HF23; -.
DR Proteomes; UP000000530; Chromosome.
DR GO; GO:0016805; F:dipeptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0008237; F:metallopeptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR InterPro; IPR036264; Bact_exopeptidase_dim_dom.
DR InterPro; IPR010964; M20A_pepV-rel.
DR InterPro; IPR002933; Peptidase_M20.
DR Pfam; PF01546; Peptidase_M20; 1.
DR SUPFAM; SSF55031; SSF55031; 1.
DR TIGRFAMs; TIGR01887; dipeptidaselike; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Dipeptidase; Hydrolase; Metal-binding; Metalloprotease;
KW Protease; Zinc.
FT CHAIN 1..469
FT /note="Putative dipeptidase SACOL1801"
FT /id="PRO_0000282625"
FT ACT_SITE 86
FT /evidence="ECO:0000250"
FT ACT_SITE 149
FT /note="Proton acceptor"
FT /evidence="ECO:0000250"
FT BINDING 84
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 115
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 115
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 150
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 173
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 440
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT HELIX 3..7
FT /evidence="ECO:0007829|PDB:3KHX"
FT HELIX 10..21
FT /evidence="ECO:0007829|PDB:3KHX"
FT STRAND 29..31
FT /evidence="ECO:0007829|PDB:3KHX"
FT STRAND 34..36
FT /evidence="ECO:0007829|PDB:3KHX"
FT HELIX 40..55
FT /evidence="ECO:0007829|PDB:3KHX"
FT STRAND 59..63
FT /evidence="ECO:0007829|PDB:3KHX"
FT TURN 64..66
FT /evidence="ECO:0007829|PDB:3KHX"
FT STRAND 67..73
FT /evidence="ECO:0007829|PDB:3KHX"
FT STRAND 78..84
FT /evidence="ECO:0007829|PDB:3KHX"
FT STRAND 105..110
FT /evidence="ECO:0007829|PDB:3KHX"
FT TURN 111..116
FT /evidence="ECO:0007829|PDB:3KHX"
FT HELIX 117..132
FT /evidence="ECO:0007829|PDB:3KHX"
FT STRAND 138..146
FT /evidence="ECO:0007829|PDB:3KHX"
FT TURN 149..151
FT /evidence="ECO:0007829|PDB:3KI9"
FT HELIX 158..162
FT /evidence="ECO:0007829|PDB:3KHX"
FT STRAND 167..170
FT /evidence="ECO:0007829|PDB:3KHX"
FT STRAND 179..182
FT /evidence="ECO:0007829|PDB:3KI9"
FT STRAND 184..192
FT /evidence="ECO:0007829|PDB:3KHX"
FT STRAND 203..211
FT /evidence="ECO:0007829|PDB:3KHX"
FT STRAND 214..217
FT /evidence="ECO:0007829|PDB:3KHZ"
FT STRAND 221..228
FT /evidence="ECO:0007829|PDB:3KHX"
FT HELIX 232..245
FT /evidence="ECO:0007829|PDB:3KHX"
FT STRAND 249..255
FT /evidence="ECO:0007829|PDB:3KHX"
FT STRAND 258..264
FT /evidence="ECO:0007829|PDB:3KHX"
FT HELIX 273..275
FT /evidence="ECO:0007829|PDB:3KHX"
FT HELIX 279..287
FT /evidence="ECO:0007829|PDB:3KHX"
FT HELIX 294..306
FT /evidence="ECO:0007829|PDB:3KHX"
FT TURN 307..309
FT /evidence="ECO:0007829|PDB:3KHX"
FT HELIX 314..316
FT /evidence="ECO:0007829|PDB:3KHX"
FT TURN 323..325
FT /evidence="ECO:0007829|PDB:3KHZ"
FT STRAND 329..338
FT /evidence="ECO:0007829|PDB:3KHX"
FT TURN 339..341
FT /evidence="ECO:0007829|PDB:3KHX"
FT STRAND 344..351
FT /evidence="ECO:0007829|PDB:3KHX"
FT HELIX 357..368
FT /evidence="ECO:0007829|PDB:3KHX"
FT HELIX 369..371
FT /evidence="ECO:0007829|PDB:3KHX"
FT STRAND 373..381
FT /evidence="ECO:0007829|PDB:3KHX"
FT HELIX 387..389
FT /evidence="ECO:0007829|PDB:3KHX"
FT HELIX 391..401
FT /evidence="ECO:0007829|PDB:3KHX"
FT STRAND 411..413
FT /evidence="ECO:0007829|PDB:3KI9"
FT HELIX 419..421
FT /evidence="ECO:0007829|PDB:3KI9"
FT STRAND 445..447
FT /evidence="ECO:0007829|PDB:3KHX"
FT HELIX 448..466
FT /evidence="ECO:0007829|PDB:3KHX"
SQ SEQUENCE 469 AA; 52824 MW; 589267D3EEC82350 CRC64;
MWKEKVQQYE DQIINDLKGL LAIESVRDDA KASEDAPVGP GPRKALDYMY EIAHRDGFTT
HDVDHIAGRI EAGKGNDVLG ILCHVDVVPA GDGWDSNPFE PVVTEDAIIA RGTLDDKGPT
IAAYYAIKIL EDMNVDWKKR IHMIIGTDEE SDWKCTDRYF KTEEMPTLGF APDAEFPCIH
GEKGITTFDL VQNKLTEDQD EPDYELITFK SGERYNMVPD HAEARVLVKE NMTDVIQDFE
YFLEQNHLQG DSTVDSGILV LTVEGKAVHG MDPSIGVNAG LYLLKFLASL NLDNNAQAFV
AFSNRYLFNS DFGEKMGMKF HTDVMGDVTT NIGVITYDNE NAGLFGINLR YPEGFEFEKA
MDRFANEIQQ YGFEVKLGKV QPPHYVDKND PFVQKLVTAY RNQTNDMTEP YTIGGGTYAR
NLDKGVAFGA MFSDSEDLMH QKNEYITKKQ LFNATSIYLE AIYSLCVEE
