ID   PEPVL_STAS1             Reviewed;         469 AA.
AC   Q49YI2;
DT   03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2005, sequence version 1.
DT   03-AUG-2022, entry version 101.
DE   RecName: Full=Putative dipeptidase SSP1012;
DE            EC=3.4.13.-;
GN   OrderedLocusNames=SSP1012;
OS   Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM
OS   20229 / NCIMB 8711 / NCTC 7292 / S-41).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=342451;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15305 / DSM 20229 / NCIMB 8711 / NCTC 7292 / S-41;
RX   PubMed=16135568; DOI=10.1073/pnas.0502950102;
RA   Kuroda M., Yamashita A., Hirakawa H., Kumano M., Morikawa K., Higashide M.,
RA   Maruyama A., Inose Y., Matoba K., Toh H., Kuhara S., Hattori M., Ohta T.;
RT   "Whole genome sequence of Staphylococcus saprophyticus reveals the
RT   pathogenesis of uncomplicated urinary tract infection.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:13272-13277(2005).
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250};
CC       Note=Binds 2 Zn(2+) ions per subunit. {ECO:0000250};
CC   -!- SIMILARITY: Belongs to the peptidase M20A family. {ECO:0000305}.
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DR   EMBL; AP008934; BAE18157.1; -; Genomic_DNA.
DR   RefSeq; WP_011302862.1; NZ_MTGA01000033.1.
DR   AlphaFoldDB; Q49YI2; -.
DR   SMR; Q49YI2; -.
DR   STRING; 342451.SSP1012; -.
DR   EnsemblBacteria; BAE18157; BAE18157; SSP1012.
DR   KEGG; ssp:SSP1012; -.
DR   PATRIC; fig|342451.11.peg.1011; -.
DR   eggNOG; COG0624; Bacteria.
DR   HOGENOM; CLU_031786_2_0_9; -.
DR   OMA; GYAGHIE; -.
DR   OrthoDB; 906744at2; -.
DR   Proteomes; UP000006371; Chromosome.
DR   GO; GO:0016805; F:dipeptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0008237; F:metallopeptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   InterPro; IPR036264; Bact_exopeptidase_dim_dom.
DR   InterPro; IPR010964; M20A_pepV-rel.
DR   InterPro; IPR002933; Peptidase_M20.
DR   Pfam; PF01546; Peptidase_M20; 1.
DR   SUPFAM; SSF55031; SSF55031; 1.
DR   TIGRFAMs; TIGR01887; dipeptidaselike; 1.
PE   3: Inferred from homology;
KW   Dipeptidase; Hydrolase; Metal-binding; Metalloprotease; Protease;
KW   Reference proteome; Zinc.
FT   CHAIN           1..469
FT                   /note="Putative dipeptidase SSP1012"
FT                   /id="PRO_0000282637"
FT   ACT_SITE        86
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        149
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   BINDING         84
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         115
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         115
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         150
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         173
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         440
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   469 AA;  52699 MW;  6B7169E305904AEA CRC64;
     MWRDKVKEYE DFILEDLKGL LSIESVREDD KASAENPVGP GPRQALDYMY KIAERDGFGT
     HDVDHIAGRI EAGKGDDVFG ILCHVDVVPA GDGWDSDPFN PVVTDDKIIA RGTLDDKGPT
     IAAYYAVKIL NEMNVNWKKR IHIIIGTDEE SDWKCTERYF QTEEMPELGF APDAEFPAIH
     GEKGISTFDV IQNEKADDQD EPEYELRSFV SGQRYNMVPD EAIANVAVKE NMTDVIQNFE
     QYLNEHDVEG ESVVDSGVLV LKVQGKAVHG MDPSIGVNAG LYLLNFLATL NLDKTAANFV
     AFSERYLFES HFGEKMGMKF HTDVMGDVTT NVGIITYDNQ DGGKFGINLR YPEGFEFEES
     LTRFKGEIQS LGFSIELGKN QTPHYVEKDD PFLQSLVQAY RNQTGDDTEP YTIGGGTYAR
     NLDKGVAFGA MFSDSEDLMH QKNEYITKKQ LFNATSIYLE SLYKLCVEE