ID   PEPX_BACAN              Reviewed;         597 AA.
AC   Q81PE9; Q6HXL3; Q6KRN8;
DT   22-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 126.
DE   RecName: Full=Putative Xaa-Pro dipeptidyl-peptidase;
DE            Short=X-Pro dipeptidyl-peptidase;
DE            EC=3.4.14.11;
DE   AltName: Full=X-prolyl-dipeptidyl aminopeptidase;
DE            Short=X-PDAP;
GN   OrderedLocusNames=BA_2860, GBAA_2860, BAS2667;
OS   Bacillus anthracis.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=1392;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Ames / isolate Porton;
RX   PubMed=12721629; DOI=10.1038/nature01586;
RA   Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T.,
RA   Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R.,
RA   Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M.,
RA   Kolonay J.F., Beanan M.J., Dodson R.J., Brinkac L.M., Gwinn M.L.,
RA   DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C.,
RA   Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y.,
RA   Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M.,
RA   Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E.,
RA   White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M.,
RA   Hanna P.C., Kolstoe A.-B., Fraser C.M.;
RT   "The genome sequence of Bacillus anthracis Ames and comparison to closely
RT   related bacteria.";
RL   Nature 423:81-86(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Ames ancestor;
RX   PubMed=18952800; DOI=10.1128/jb.01347-08;
RA   Ravel J., Jiang L., Stanley S.T., Wilson M.R., Decker R.S., Read T.D.,
RA   Worsham P., Keim P.S., Salzberg S.L., Fraser-Liggett C.M., Rasko D.A.;
RT   "The complete genome sequence of Bacillus anthracis Ames 'Ancestor'.";
RL   J. Bacteriol. 191:445-446(2009).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Sterne;
RA   Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K.,
RA   Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R.,
RA   Richardson P., Rubin E., Tice H.;
RT   "Complete genome sequence of Bacillus anthracis Sterne.";
RL   Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolyzes Xaa-Pro-|- bonds to release unblocked, N-terminal
CC         dipeptides from substrates including Ala-Pro-|-p-nitroanilide and
CC         (sequentially) Tyr-Pro-|-Phe-Pro-|-Gly-Pro-|-Ile.; EC=3.4.14.11;
CC   -!- SIMILARITY: Belongs to the peptidase S15 family. {ECO:0000305}.
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DR   EMBL; AE016879; AAP26688.1; -; Genomic_DNA.
DR   EMBL; AE017334; AAT31978.1; -; Genomic_DNA.
DR   EMBL; AE017225; AAT54976.1; -; Genomic_DNA.
DR   RefSeq; NP_845202.1; NC_003997.3.
DR   RefSeq; WP_000516719.1; NZ_WXXJ01000020.1.
DR   RefSeq; YP_028925.1; NC_005945.1.
DR   AlphaFoldDB; Q81PE9; -.
DR   SMR; Q81PE9; -.
DR   IntAct; Q81PE9; 1.
DR   STRING; 260799.BAS2667; -.
DR   ESTHER; baccr-pepx; Lactobacillus_peptidase.
DR   DNASU; 1088416; -.
DR   EnsemblBacteria; AAP26688; AAP26688; BA_2860.
DR   EnsemblBacteria; AAT31978; AAT31978; GBAA_2860.
DR   GeneID; 45022692; -.
DR   KEGG; ban:BA_2860; -.
DR   KEGG; bar:GBAA_2860; -.
DR   KEGG; bat:BAS2667; -.
DR   PATRIC; fig|198094.11.peg.2844; -.
DR   eggNOG; COG2936; Bacteria.
DR   HOGENOM; CLU_011800_1_0_9; -.
DR   OMA; WDKIKNW; -.
DR   Proteomes; UP000000427; Chromosome.
DR   Proteomes; UP000000594; Chromosome.
DR   GO; GO:0004177; F:aminopeptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0008239; F:dipeptidyl-peptidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0008236; F:serine-type peptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.1820; -; 2.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR005674; CocE/Ser_esterase.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR008252; Pept_S15_Xpro.
DR   InterPro; IPR000383; Xaa-Pro-like_dom.
DR   InterPro; IPR013736; Xaa-Pro_dipept_C.
DR   Pfam; PF02129; Peptidase_S15; 1.
DR   Pfam; PF08530; PepX_C; 1.
DR   PRINTS; PR00923; LACTOPTASE.
DR   SMART; SM00939; PepX_C; 1.
DR   SUPFAM; SSF49785; SSF49785; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
DR   TIGRFAMs; TIGR00976; NonD; 1.
PE   3: Inferred from homology;
KW   Aminopeptidase; Hydrolase; Protease; Reference proteome; Serine protease.
FT   CHAIN           1..597
FT                   /note="Putative Xaa-Pro dipeptidyl-peptidase"
FT                   /id="PRO_0000220236"
FT   ACT_SITE        224
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        336
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        367
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   597 AA;  66296 MW;  F203893D24166E18 CRC64;
     MGKKKIATLS AILSLSITSG VSSTTAYADN KETNYINEND VKLNGKVSEA LQSNMKIELE
     NGMTKPIYSL DEAIIENLFV ETEVDSDRDG KKDRVSVKVM RPKTDSNVKV PVIYEMSPYR
     AGLKDVPVYN VDEELYAYEG KPYGAVNLGS YGNYYVPRGY AVILGESIGT GKSEGCPTTG
     DEQEILGTKS VIDWVNGRAK AYKENGEEVK ADWSTGNVGM TGVSYNGTLP NAVATTGVEG
     LKTIIPIAAI SSWYDYYRAN GAVIAPGGYQ GEDTDNMAEA VLTRENPEVC GQVIKELTAG
     QDRKTGNYND FWDKRNYVKD AKNVKASVFV VHGLNDWNVK TKQFAQWWEA LGENNVPRKM
     WLHQGGHGGT STNDWQRTQN KWFDYWLYGI ENGIMNEPMV DVQRENKTWD KMKNWPDPSA
     VPSKVRMYLS NKAVNLPLSM GSVNKMFSFV DDAKMKSNQL VANPELEVAN RLVYTMPVLQ
     KDTRISGTPK ISITGNIDRS VSNLTALLVD YGGAKPEIVT RGWMDPQNVK SIENSTAIQP
     GKDYTFTWDM QPDDYVFKAG HQIGVVLMAS DYDYTIRPKA GTKLTVKLSE VTLPIVK