PEPX_LACHE
ID PEPX_LACHE Reviewed; 793 AA.
AC Q59485; Q48571;
DT 22-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Xaa-Pro dipeptidyl-peptidase {ECO:0000255|HAMAP-Rule:MF_00698};
DE EC=3.4.14.11 {ECO:0000255|HAMAP-Rule:MF_00698};
DE AltName: Full=X-Pro dipeptidyl-peptidase {ECO:0000255|HAMAP-Rule:MF_00698};
DE AltName: Full=X-prolyl-dipeptidyl aminopeptidase {ECO:0000255|HAMAP-Rule:MF_00698};
DE Short=X-PDAP {ECO:0000255|HAMAP-Rule:MF_00698};
GN Name=pepX {ECO:0000255|HAMAP-Rule:MF_00698};
OS Lactobacillus helveticus (Lactobacillus suntoryeus).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC Lactobacillus.
OX NCBI_TaxID=1587;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=CNRZ 32;
RX PubMed=8867635; DOI=10.1007/bf00178616;
RA Yuksel G.U., Steele J.L.;
RT "DNA sequence analysis, expression, distribution, and physiological role of
RT the Xaa-prolyldipeptidyl aminopeptidase gene from Lactobacillus helveticus
RT CNRZ32.";
RL Appl. Microbiol. Biotechnol. 44:766-773(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND SUBUNIT.
RX PubMed=8574400; DOI=10.1099/13500872-141-12-3067;
RA Vesanto E., Savijoki K., Rantanen T., Steele J.L., Palva A.;
RT "An X-prolyl dipeptidyl aminopeptidase (pepX) gene from Lactobacillus
RT helveticus.";
RL Microbiology 141:3067-3075(1995).
CC -!- FUNCTION: Removes N-terminal dipeptides sequentially from polypeptides
CC having unsubstituted N-termini provided that the penultimate residue is
CC proline.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolyzes Xaa-Pro-|- bonds to release unblocked, N-terminal
CC dipeptides from substrates including Ala-Pro-|-p-nitroanilide and
CC (sequentially) Tyr-Pro-|-Phe-Pro-|-Gly-Pro-|-Ile.; EC=3.4.14.11;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00698};
CC -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_00698,
CC ECO:0000269|PubMed:8574400}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00698}.
CC -!- SIMILARITY: Belongs to the peptidase S15 family. {ECO:0000255|HAMAP-
CC Rule:MF_00698}.
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DR EMBL; U22900; AAB50275.1; -; Genomic_DNA.
DR EMBL; Z48236; CAA88273.1; -; Genomic_DNA.
DR RefSeq; WP_003629197.1; NZ_SCLV01000151.1.
DR PDB; 6NFF; X-ray; 2.00 A; A=1-793.
DR PDBsum; 6NFF; -.
DR AlphaFoldDB; Q59485; -.
DR SMR; Q59485; -.
DR STRING; 326425.lhe_1355; -.
DR ESTHER; lache-pepx; Lactobacillus_peptidase.
DR MEROPS; S15.001; -.
DR eggNOG; COG2936; Bacteria.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004177; F:aminopeptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0008239; F:dipeptidyl-peptidase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008236; F:serine-type peptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.1820; -; 1.
DR HAMAP; MF_00698; Aminopeptidase_S15; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR005674; CocE/Ser_esterase.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR008252; Pept_S15_Xpro.
DR InterPro; IPR015251; PepX_N_dom.
DR InterPro; IPR036313; PepX_N_dom_sf.
DR InterPro; IPR000383; Xaa-Pro-like_dom.
DR InterPro; IPR013736; Xaa-Pro_dipept_C.
DR Pfam; PF02129; Peptidase_S15; 1.
DR Pfam; PF08530; PepX_C; 1.
DR Pfam; PF09168; PepX_N; 1.
DR PRINTS; PR00923; LACTOPTASE.
DR SMART; SM00939; PepX_C; 1.
DR SMART; SM00940; PepX_N; 1.
DR SUPFAM; SSF49785; SSF49785; 1.
DR SUPFAM; SSF53474; SSF53474; 1.
DR SUPFAM; SSF81761; SSF81761; 1.
DR TIGRFAMs; TIGR00976; NonD; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Aminopeptidase; Cytoplasm; Hydrolase; Protease;
KW Serine protease.
FT CHAIN 1..793
FT /note="Xaa-Pro dipeptidyl-peptidase"
FT /id="PRO_0000220219"
FT ACT_SITE 363
FT /note="Charge relay system"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00698"
FT ACT_SITE 483
FT /note="Charge relay system"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00698"
FT ACT_SITE 514
FT /note="Charge relay system"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00698"
FT CONFLICT 85
FT /note="K -> E (in Ref. 2; CAA88273)"
FT /evidence="ECO:0000305"
FT CONFLICT 120
FT /note="L -> V (in Ref. 2; CAA88273)"
FT /evidence="ECO:0000305"
FT CONFLICT 259
FT /note="V -> I (in Ref. 2; CAA88273)"
FT /evidence="ECO:0000305"
FT CONFLICT 385
FT /note="S -> A (in Ref. 2; CAA88273)"
FT /evidence="ECO:0000305"
FT CONFLICT 520
FT /note="L -> F (in Ref. 2; CAA88273)"
FT /evidence="ECO:0000305"
FT CONFLICT 528
FT /note="L -> F (in Ref. 2; CAA88273)"
FT /evidence="ECO:0000305"
FT CONFLICT 640
FT /note="T -> I (in Ref. 2; CAA88273)"
FT /evidence="ECO:0000305"
FT CONFLICT 686
FT /note="H -> R (in Ref. 2; CAA88273)"
FT /evidence="ECO:0000305"
FT HELIX 13..22
FT /evidence="ECO:0007829|PDB:6NFF"
FT HELIX 30..32
FT /evidence="ECO:0007829|PDB:6NFF"
FT HELIX 35..46
FT /evidence="ECO:0007829|PDB:6NFF"
FT HELIX 53..59
FT /evidence="ECO:0007829|PDB:6NFF"
FT HELIX 60..62
FT /evidence="ECO:0007829|PDB:6NFF"
FT STRAND 64..66
FT /evidence="ECO:0007829|PDB:6NFF"
FT HELIX 71..76
FT /evidence="ECO:0007829|PDB:6NFF"
FT HELIX 84..94
FT /evidence="ECO:0007829|PDB:6NFF"
FT TURN 100..102
FT /evidence="ECO:0007829|PDB:6NFF"
FT HELIX 108..114
FT /evidence="ECO:0007829|PDB:6NFF"
FT HELIX 126..137
FT /evidence="ECO:0007829|PDB:6NFF"
FT STRAND 144..146
FT /evidence="ECO:0007829|PDB:6NFF"
FT HELIX 147..153
FT /evidence="ECO:0007829|PDB:6NFF"
FT HELIX 156..160
FT /evidence="ECO:0007829|PDB:6NFF"
FT HELIX 179..181
FT /evidence="ECO:0007829|PDB:6NFF"
FT STRAND 183..190
FT /evidence="ECO:0007829|PDB:6NFF"
FT STRAND 198..200
FT /evidence="ECO:0007829|PDB:6NFF"
FT STRAND 203..210
FT /evidence="ECO:0007829|PDB:6NFF"
FT HELIX 212..216
FT /evidence="ECO:0007829|PDB:6NFF"
FT STRAND 220..226
FT /evidence="ECO:0007829|PDB:6NFF"
FT TURN 228..231
FT /evidence="ECO:0007829|PDB:6NFF"
FT HELIX 237..239
FT /evidence="ECO:0007829|PDB:6NFF"
FT HELIX 250..252
FT /evidence="ECO:0007829|PDB:6NFF"
FT HELIX 287..293
FT /evidence="ECO:0007829|PDB:6NFF"
FT TURN 294..296
FT /evidence="ECO:0007829|PDB:6NFF"
FT STRAND 298..302
FT /evidence="ECO:0007829|PDB:6NFF"
FT HELIX 319..332
FT /evidence="ECO:0007829|PDB:6NFF"
FT STRAND 338..341
FT /evidence="ECO:0007829|PDB:6NFF"
FT STRAND 353..359
FT /evidence="ECO:0007829|PDB:6NFF"
FT HELIX 364..374
FT /evidence="ECO:0007829|PDB:6NFF"
FT STRAND 380..387
FT /evidence="ECO:0007829|PDB:6NFF"
FT HELIX 393..396
FT /evidence="ECO:0007829|PDB:6NFF"
FT HELIX 413..419
FT /evidence="ECO:0007829|PDB:6NFF"
FT HELIX 422..425
FT /evidence="ECO:0007829|PDB:6NFF"
FT HELIX 427..448
FT /evidence="ECO:0007829|PDB:6NFF"
FT TURN 450..452
FT /evidence="ECO:0007829|PDB:6NFF"
FT HELIX 457..460
FT /evidence="ECO:0007829|PDB:6NFF"
FT HELIX 464..470
FT /evidence="ECO:0007829|PDB:6NFF"
FT STRAND 473..480
FT /evidence="ECO:0007829|PDB:6NFF"
FT STRAND 484..486
FT /evidence="ECO:0007829|PDB:6NFF"
FT HELIX 489..498
FT /evidence="ECO:0007829|PDB:6NFF"
FT STRAND 501..503
FT /evidence="ECO:0007829|PDB:6NFF"
FT STRAND 505..511
FT /evidence="ECO:0007829|PDB:6NFF"
FT STRAND 520..522
FT /evidence="ECO:0007829|PDB:6NFF"
FT HELIX 525..538
FT /evidence="ECO:0007829|PDB:6NFF"
FT HELIX 544..546
FT /evidence="ECO:0007829|PDB:6NFF"
FT STRAND 550..554
FT /evidence="ECO:0007829|PDB:6NFF"
FT STRAND 562..566
FT /evidence="ECO:0007829|PDB:6NFF"
FT HELIX 570..572
FT /evidence="ECO:0007829|PDB:6NFF"
FT STRAND 573..580
FT /evidence="ECO:0007829|PDB:6NFF"
FT STRAND 584..587
FT /evidence="ECO:0007829|PDB:6NFF"
FT STRAND 595..600
FT /evidence="ECO:0007829|PDB:6NFF"
FT HELIX 604..609
FT /evidence="ECO:0007829|PDB:6NFF"
FT HELIX 613..622
FT /evidence="ECO:0007829|PDB:6NFF"
FT HELIX 625..628
FT /evidence="ECO:0007829|PDB:6NFF"
FT STRAND 631..635
FT /evidence="ECO:0007829|PDB:6NFF"
FT STRAND 643..660
FT /evidence="ECO:0007829|PDB:6NFF"
FT STRAND 663..674
FT /evidence="ECO:0007829|PDB:6NFF"
FT STRAND 682..695
FT /evidence="ECO:0007829|PDB:6NFF"
FT STRAND 697..702
FT /evidence="ECO:0007829|PDB:6NFF"
FT STRAND 709..720
FT /evidence="ECO:0007829|PDB:6NFF"
FT HELIX 721..723
FT /evidence="ECO:0007829|PDB:6NFF"
FT STRAND 724..726
FT /evidence="ECO:0007829|PDB:6NFF"
FT STRAND 739..744
FT /evidence="ECO:0007829|PDB:6NFF"
FT STRAND 748..752
FT /evidence="ECO:0007829|PDB:6NFF"
FT STRAND 757..764
FT /evidence="ECO:0007829|PDB:6NFF"
FT TURN 767..769
FT /evidence="ECO:0007829|PDB:6NFF"
FT STRAND 777..793
FT /evidence="ECO:0007829|PDB:6NFF"
SQ SEQUENCE 793 AA; 90487 MW; 585F6F0DB08B82A9 CRC64;
MKYNQYAYVE TDFQQQVKEL IDINFLPKNY QVWDFSSLLA KLVKNAIAEA KTDAAKNAKL
AEFAVSDHQT LADFLKEKPT EIGTKQFYNV ALQLLGYHVH YDYDFADPTG FMQRNALPFL
QDISDNQKLI SAFYRLLNTR AKNGQILLDV MAGKGYFTQF WGQNKFKFFN GKSIPVFDTN
KVIREVVYVE TDLDTDHDGK SDLIQVTVFR PEETNKGLKV PALYTASPYF GGIIANEKRN
HNVDENLSDS TEWNDPQYVH SPIVKAEKPD GSSRPATEEA VHKSSYPLNE YMLARGFASV
FAGAIGTRGS DGVRITGAPE ETESAAAVIE WLHGDRVAYT DRTRTVQTTA DWCNGNIGMT
GRSYLGTLQI AIATTGVKGL KTVVSEAAIS SWYDYYREHG LVIAPEACQG EDLDLLAETC
QSNLWDAGSY LKIKPEYDKM QKQLREKEDR NTGQYSDFWE ARNYRHHADG IKCSWISVHG
LNDWNVKPKN VYKIWQLVKK MPMKHHLFLH QGPHYNMNNL VSIDFTDLMN LWFVHELLGI
ENNAYNQWPT VMIQDNLQAD KWHEEPDWSN DLGQEKIYYP TDEGELFQDG NGKAQKSFTD
VGGIEFKKAG ISESDWQYKF ICGDEKWAKP SLRFETDEFT HPTTIVGRPE VKVRVSASLP
KGEISVALVE LGERQRLTAT PKFLMHGGQE LGYRFGTDTL QEFVPDKKTK AKLITKAHMN
LQNFKDMKKP EAIDADKFYD LDFLLQPTYY TIPSGSKLAL IIYSTDQGMT KRPLEDETYT
IDLANTEIKF YEK
