PER33_YEAST
ID PER33_YEAST Reviewed; 273 AA.
AC Q12144; D6VY65;
DT 25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Pore and endoplasmic reticulum protein of 33 kDa;
GN Name=PER33; OrderedLocusNames=YLR064W; ORFNames=L2177;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169871;
RA Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W.,
RA Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A.,
RA Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K.,
RA Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., Koetter P., Louis E.J.,
RA Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S.,
RA Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D.,
RA Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M.,
RA Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P.,
RA Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M.,
RA Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K.,
RA Zollner A., Hani J., Hoheisel J.D.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
RL Nature 387:87-90(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
RN [7]
RP SUBCELLULAR LOCATION.
RX PubMed=20498018; DOI=10.1083/jcb.200910043;
RA Chadrin A., Hess B., San Roman M., Gatti X., Lombard B., Loew D.,
RA Barral Y., Palancade B., Doye V.;
RT "Pom33, a novel transmembrane nucleoporin required for proper nuclear pore
RT complex distribution.";
RL J. Cell Biol. 189:795-811(2010).
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass
CC membrane protein. Nucleus, nuclear pore complex.
CC -!- MISCELLANEOUS: Present with 2240 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the PER33/POM33 family. {ECO:0000305}.
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DR EMBL; X94607; CAA64310.1; -; Genomic_DNA.
DR EMBL; Z73236; CAA97620.1; -; Genomic_DNA.
DR EMBL; AY692677; AAT92696.1; -; Genomic_DNA.
DR EMBL; BK006945; DAA09381.1; -; Genomic_DNA.
DR PIR; S61637; S61637.
DR RefSeq; NP_013165.1; NM_001181951.1.
DR AlphaFoldDB; Q12144; -.
DR SMR; Q12144; -.
DR BioGRID; 31338; 202.
DR DIP; DIP-4778N; -.
DR IntAct; Q12144; 3.
DR MINT; Q12144; -.
DR STRING; 4932.YLR064W; -.
DR MaxQB; Q12144; -.
DR PaxDb; Q12144; -.
DR PRIDE; Q12144; -.
DR EnsemblFungi; YLR064W_mRNA; YLR064W; YLR064W.
DR GeneID; 850753; -.
DR KEGG; sce:YLR064W; -.
DR SGD; S000004054; PER33.
DR VEuPathDB; FungiDB:YLR064W; -.
DR eggNOG; KOG4002; Eukaryota.
DR GeneTree; ENSGT00390000011368; -.
DR HOGENOM; CLU_065417_3_0_1; -.
DR InParanoid; Q12144; -.
DR OMA; PQFYWFL; -.
DR BioCyc; YEAST:G3O-32217-MON; -.
DR PRO; PR:Q12144; -.
DR Proteomes; UP000002311; Chromosome XII.
DR RNAct; Q12144; protein.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:SGD.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005635; C:nuclear envelope; IDA:SGD.
DR GO; GO:0005643; C:nuclear pore; IEA:UniProtKB-SubCell.
DR GO; GO:0071786; P:endoplasmic reticulum tubular network organization; IBA:GO_Central.
DR GO; GO:0061024; P:membrane organization; IBA:GO_Central.
DR GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR InterPro; IPR005344; TMEM33/Pom33.
DR Pfam; PF03661; TMEM33_Pom33; 1.
PE 1: Evidence at protein level;
KW Endoplasmic reticulum; Membrane; mRNA transport; Nuclear pore complex;
KW Nucleus; Protein transport; Reference proteome; Translocation;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..273
FT /note="Pore and endoplasmic reticulum protein of 33 kDa"
FT /id="PRO_0000247231"
FT TOPO_DOM 1..24
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 25..45
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 46..56
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 57..74
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 75..98
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 99..119
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 120..126
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 127..147
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 148..176
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 177..197
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 198..199
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 200..220
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 221..273
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 273 AA; 32637 MW; F6B7E3A3D1D74F38 CRC64;
MTVPRNRPMA PFGTIIKSRI KQPQFYWFIG HFLTIFNFIQ FHLSITSKQN QLSCYRRSLF
YISVTYAIVL YQFFKSDQLK FNFTLLRQEM KKLDNLQYFA MLFILFLLSQ FNIIISGSLY
SPVIFSIFHF LNYFKENLLP FLPLIPLNLK NLLNSKITVF IQNYNGFFLQ MAQVFEIICG
LRVGLFLVPF NFFLLLVRRA NVSFEVVGTM LAGLTYVWFF KLRYLQSESM RQIFKQYVLR
LDAYVSRTLP PYCSRLWNGY KNFVMTVFWK IPV
