ID   A49_VACCW               Reviewed;         162 AA.
AC   P31037; Q76ZM9;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 1.
DT   23-FEB-2022, entry version 61.
DE   RecName: Full=Protein A49R;
GN   OrderedLocusNames=VACWR175; ORFNames=A49R;
OS   Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain
OS   WR)).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=10254;
OH   NCBI_TaxID=9913; Bos taurus (Bovine).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2045793; DOI=10.1099/0022-1317-72-6-1349;
RA   Smith G.L., Chan Y.S., Howard S.T.;
RT   "Nucleotide sequence of 42 kbp of vaccinia virus strain WR from near the
RT   right inverted terminal repeat.";
RL   J. Gen. Virol. 72:1349-1376(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J.,
RA   Wohlhueter R.;
RT   "Sequencing of the coding region of Vaccinia-WR to an average 9-fold
RT   redundancy and an error rate of 0.16/10kb.";
RL   Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   FUNCTION, INTERACTION WITH HOST BTRC, AND SUBCELLULAR LOCATION.
RX   PubMed=23468625; DOI=10.1371/journal.ppat.1003183;
RA   Mansur D.S., Maluquer de Motes C., Unterholzner L., Sumner R.P.,
RA   Ferguson B.J., Ren H., Strnadova P., Bowie A.G., Smith G.L.;
RT   "Poxvirus targeting of E3 ligase beta-TrCP by molecular mimicry: a
RT   mechanism to inhibit NF-kappaB activation and promote immune evasion and
RT   virulence.";
RL   PLoS Pathog. 9:E1003183-E1003183(2013).
CC   -!- FUNCTION: Plays a role in the inhibition of host NF-kappa-B activation.
CC       Interacts with host BTRC and thereby diminishes ubiquitination of NF-
CC       kappa-B inhibitor alpha/NFKBIA. This stabilizes NFKBIA and its
CC       interaction with NF-kappaB, so retaining p65/RELA in the cytoplasm and
CC       preventing NF-kappa-B-dependent gene expression.
CC       {ECO:0000269|PubMed:23468625}.
CC   -!- SUBUNIT: Interacts with host BTRC; this interaction inhibits NF-kappa-B
CC       activation. {ECO:0000269|PubMed:23468625}.
CC   -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000269|PubMed:23468625}.
CC       Host nucleus {ECO:0000269|PubMed:23468625}.
CC   -!- SIMILARITY: Belongs to the poxviridae A49 protein family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; D11079; BAA01823.1; -; Genomic_DNA.
DR   EMBL; AY243312; AAO89454.1; -; Genomic_DNA.
DR   PIR; JQ1787; JQ1787.
DR   RefSeq; YP_233057.1; NC_006998.1.
DR   PDB; 4D5R; X-ray; 1.90 A; A/B=13-162.
DR   PDB; 4D5S; X-ray; 3.00 A; A/B=1-162.
DR   PDB; 4D5T; X-ray; 1.84 A; A/B/C/D/E/F/G/H=13-162.
DR   PDBsum; 4D5R; -.
DR   PDBsum; 4D5S; -.
DR   PDBsum; 4D5T; -.
DR   SMR; P31037; -.
DR   BioGRID; 3509058; 1.
DR   DIP; DIP-2163N; -.
DR   IntAct; P31037; 1.
DR   MINT; P31037; -.
DR   DNASU; 3707704; -.
DR   GeneID; 3707704; -.
DR   KEGG; vg:3707704; -.
DR   Proteomes; UP000000344; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0039644; P:suppression by virus of host NF-kappaB cascade; IEA:UniProtKB-KW.
DR   InterPro; IPR009473; Orthopox_A49.
DR   Pfam; PF06489; Orthopox_A49R; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Host cytoplasm; Host nucleus; Host-virus interaction;
KW   Inhibition of host NF-kappa-B by virus; Reference proteome.
FT   CHAIN           1..162
FT                   /note="Protein A49R"
FT                   /id="PRO_0000099343"
FT   HELIX           20..34
FT                   /evidence="ECO:0007829|PDB:4D5T"
FT   HELIX           35..37
FT                   /evidence="ECO:0007829|PDB:4D5T"
FT   HELIX           40..51
FT                   /evidence="ECO:0007829|PDB:4D5T"
FT   HELIX           60..72
FT                   /evidence="ECO:0007829|PDB:4D5T"
FT   HELIX           75..83
FT                   /evidence="ECO:0007829|PDB:4D5T"
FT   HELIX           87..98
FT                   /evidence="ECO:0007829|PDB:4D5T"
FT   HELIX           99..102
FT                   /evidence="ECO:0007829|PDB:4D5T"
FT   HELIX           106..120
FT                   /evidence="ECO:0007829|PDB:4D5T"
FT   HELIX           123..137
FT                   /evidence="ECO:0007829|PDB:4D5T"
FT   HELIX           143..161
FT                   /evidence="ECO:0007829|PDB:4D5T"
SQ   SEQUENCE   162 AA;  18754 MW;  EE91411C975097C3 CRC64;
     MDEAYYSGNL ESVLGYVSDM HTELASISQL VIAKIETIDN DILNKDIVNF IMCRSNLDNP
     FISFLDTVYT IIDQENYQTE LINSLDDNEI IDCIVNKFMS FYKDNLENIV DAIITLKYIM
     NNPDFKTTYA EVLGSRIADI DIKQVIRENI LQLSNDIRER YL