PES4_YEAST
ID PES4_YEAST Reviewed; 611 AA.
AC P39684; D6VTQ3;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 2.
DT 03-AUG-2022, entry version 166.
DE RecName: Full=Protein PES4;
DE AltName: Full=DNA polymerase epsilon suppressor 4;
GN Name=PES4; OrderedLocusNames=YFR023W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=SK1;
RA Araki H.;
RL Submitted (JAN-1994) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7670463; DOI=10.1038/ng0795-261;
RA Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S.,
RA Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H.,
RA Eki T.;
RT "Analysis of the nucleotide sequence of chromosome VI from Saccharomyces
RT cerevisiae.";
RL Nat. Genet. 10:261-268(1995).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
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DR EMBL; D26442; BAA05461.1; -; Genomic_DNA.
DR EMBL; D50617; BAA09262.1; -; Genomic_DNA.
DR EMBL; BK006940; DAA12463.1; -; Genomic_DNA.
DR PIR; S56278; S56278.
DR RefSeq; NP_116678.1; NM_001179988.1.
DR PDB; 6EXX; X-ray; 1.10 A; A=391-469.
DR PDB; 6EZ7; X-ray; 1.90 A; A=303-384.
DR PDBsum; 6EXX; -.
DR PDBsum; 6EZ7; -.
DR AlphaFoldDB; P39684; -.
DR SMR; P39684; -.
DR BioGRID; 31176; 82.
DR STRING; 4932.YFR023W; -.
DR PaxDb; P39684; -.
DR PRIDE; P39684; -.
DR EnsemblFungi; YFR023W_mRNA; YFR023W; YFR023W.
DR GeneID; 850579; -.
DR KEGG; sce:YFR023W; -.
DR SGD; S000001919; PES4.
DR VEuPathDB; FungiDB:YFR023W; -.
DR eggNOG; KOG0123; Eukaryota.
DR GeneTree; ENSGT00940000176742; -.
DR HOGENOM; CLU_020939_0_0_1; -.
DR InParanoid; P39684; -.
DR OMA; LCGIHFD; -.
DR BioCyc; YEAST:G3O-30474-MON; -.
DR Reactome; R-SCE-156827; L13a-mediated translational silencing of Ceruloplasmin expression.
DR Reactome; R-SCE-975956; Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
DR Reactome; R-SCE-975957; Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
DR PRO; PR:P39684; -.
DR Proteomes; UP000002311; Chromosome VI.
DR RNAct; P39684; protein.
DR GO; GO:0010494; C:cytoplasmic stress granule; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005628; C:prospore membrane; IDA:SGD.
DR GO; GO:1990904; C:ribonucleoprotein complex; IBA:GO_Central.
DR GO; GO:0003730; F:mRNA 3'-UTR binding; IBA:GO_Central.
DR GO; GO:0008143; F:poly(A) binding; IBA:GO_Central.
DR GO; GO:0008266; F:poly(U) RNA binding; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0010609; P:mRNA localization resulting in post-transcriptional regulation of gene expression; IGI:SGD.
DR GO; GO:0016071; P:mRNA metabolic process; IGI:SGD.
DR GO; GO:0006417; P:regulation of translation; IGI:SGD.
DR Gene3D; 3.30.70.330; -; 4.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR InterPro; IPR003954; RRM_dom_euk.
DR Pfam; PF00076; RRM_1; 3.
DR SMART; SM00360; RRM; 4.
DR SMART; SM00361; RRM_1; 3.
DR SUPFAM; SSF54928; SSF54928; 2.
DR PROSITE; PS50102; RRM; 4.
PE 1: Evidence at protein level;
KW 3D-structure; Nucleus; Reference proteome; Repeat; RNA-binding.
FT CHAIN 1..611
FT /note="Protein PES4"
FT /id="PRO_0000081724"
FT DOMAIN 91..169
FT /note="RRM 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 179..247
FT /note="RRM 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 303..379
FT /note="RRM 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 393..471
FT /note="RRM 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 37..81
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 58..78
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 124
FT /note="L -> I (in Ref. 1; BAA05461)"
FT /evidence="ECO:0000305"
FT CONFLICT 541..611
FT /note="QVRRGIDFMRFPSATRDENVHGIAEYIFDTYWNRDVLILDKFLSLLNSSPYH
FT EGVLQKQIEEAASSLGFKR -> ASKKRYRFHEISKCH (in Ref. 1;
FT BAA05461)"
FT /evidence="ECO:0000305"
FT STRAND 304..310
FT /evidence="ECO:0007829|PDB:6EZ7"
FT HELIX 316..323
FT /evidence="ECO:0007829|PDB:6EZ7"
FT STRAND 329..334
FT /evidence="ECO:0007829|PDB:6EZ7"
FT STRAND 342..351
FT /evidence="ECO:0007829|PDB:6EZ7"
FT HELIX 352..362
FT /evidence="ECO:0007829|PDB:6EZ7"
FT STRAND 373..376
FT /evidence="ECO:0007829|PDB:6EZ7"
FT STRAND 393..400
FT /evidence="ECO:0007829|PDB:6EXX"
FT HELIX 406..415
FT /evidence="ECO:0007829|PDB:6EXX"
FT STRAND 421..429
FT /evidence="ECO:0007829|PDB:6EXX"
FT TURN 430..433
FT /evidence="ECO:0007829|PDB:6EXX"
FT STRAND 434..443
FT /evidence="ECO:0007829|PDB:6EXX"
FT HELIX 444..454
FT /evidence="ECO:0007829|PDB:6EXX"
FT STRAND 465..468
FT /evidence="ECO:0007829|PDB:6EXX"
SQ SEQUENCE 611 AA; 70842 MW; 78519E76A8058701 CRC64;
MYSISNKKPS ILSMVPLNIL KNQDLKVKKD QEKKISFNPV VTPIRPDDYH EKTSRSSSSS
HSDSPEFLRI NNNKSGHKNG KLKSFESKKL VPLFIGDLHE TVTEETLKGI FKKYPSFVSA
KVCLDSVTKK SLGHGYLNFE DKEEAEKAME ELNYTKVNGK EIRIMPSLRN TTFRKNFGTN
VFFSNLPLNN PLLTTRVFYD TFSRYGKILS CKLDSRKDIG FVYFEDEKTA RNVIKMYNNT
SFFGKKILCG IHFDKEVRSV PNFETQKSRL DAETIIEKEQ SLNEKHSKGN DKESKNIYSS
SQNSIFIKNL PTITTRDDIL NFFSEVGPIK SIYLSNATKV KYLWAFVTYK NSSDSEKAIK
RYNNFYFRGK KLLVTRAQDK EERAKFIESQ KISTLFLENL SAVCNKEFLK YLCHQENIRP
FKIQIDGYDE NSSTYSGFIK FRNFEDATRI FNFLNNRLVG GSIVTTSWER QNNAPKYHDG
YGMRNIHTSS HPQITPYYQY SHANSLNSPH MRDLSSMNSS TRSLIKNKNF NKKVLETFEK
QVRRGIDFMR FPSATRDENV HGIAEYIFDT YWNRDVLILD KFLSLLNSSP YHEGVLQKQI
EEAASSLGFK R
