PESC_DANRE
ID PESC_DANRE Reviewed; 583 AA.
AC P79741; A2BGH4; B1H1I9; Q7ZU09; Q9I906;
DT 26-JUL-2002, integrated into UniProtKB/Swiss-Prot.
DT 05-MAY-2009, sequence version 2.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Pescadillo;
GN Name=pes; ORFNames=si:dkeyp-86g2.3;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=8985183; DOI=10.1101/gad.10.24.3141;
RA Allende M.L., Amsterdam A., Becker T., Kawakami K., Gaiano N., Hopkins N.;
RT "Insertional mutagenesis in zebrafish identifies two novel genes,
RT pescadillo and dead eye, essential for embryonic development.";
RL Genes Dev. 10:3141-3155(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-180.
RA Kawakami K., Grosshans H., Hopkins N.;
RT "The genomic sequence of the zebrafish pescadillo gene.";
RL Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Component of the PeBoW complex, which is required for
CC maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S
CC ribosome. {ECO:0000255|HAMAP-Rule:MF_03028}.
CC -!- SUBUNIT: Component of the PeBoW complex, composed of bop1, pes1 and
CC wdr12. The complex is held together by bop1, which interacts with pes1
CC via its N-terminal domain and with wdr12 via a high-affinity
CC interaction between the seven-bladed beta-propeller domains of the 2
CC proteins. The PeBoW complex associates with the 66S pre-ribosome.
CC {ECO:0000255|HAMAP-Rule:MF_03028}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000255|HAMAP-
CC Rule:MF_03028}. Nucleus, nucleoplasm {ECO:0000255|HAMAP-Rule:MF_03028}.
CC -!- DEVELOPMENTAL STAGE: Widely and highly expressed during the first 3
CC days of embryogenesis. Prominent sites of expression are the eyes and
CC optic tectum on day 1, the fin buds, liver primordium, and gut on day
CC 2, and the branchial arches on day 3. {ECO:0000269|PubMed:8985183}.
CC -!- DISRUPTION PHENOTYPE: Embryos exhibit smaller eyes, a reduced brain and
CC visceral skeleton, shortened fins and a lack of expansion of liver and
CC gut, and die on day 6 of development. {ECO:0000269|PubMed:8985183}.
CC -!- SIMILARITY: Belongs to the pescadillo family. {ECO:0000255|HAMAP-
CC Rule:MF_03028}.
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DR EMBL; U77627; AAB61138.1; -; mRNA.
DR EMBL; BX470167; CAM16783.2; -; Genomic_DNA.
DR EMBL; CU459127; CAM16783.2; JOINED; Genomic_DNA.
DR EMBL; CU459127; CAQ14501.1; -; Genomic_DNA.
DR EMBL; BX470167; CAQ14501.1; JOINED; Genomic_DNA.
DR EMBL; AB046115; BAB01764.1; -; Genomic_DNA.
DR EMBL; BC049339; AAH49339.1; -; mRNA.
DR EMBL; BC160624; AAI60624.1; -; mRNA.
DR RefSeq; NP_571105.3; NM_131030.3.
DR AlphaFoldDB; P79741; -.
DR SMR; P79741; -.
DR STRING; 7955.ENSDARP00000026992; -.
DR PaxDb; P79741; -.
DR Ensembl; ENSDART00000023101; ENSDARP00000026992; ENSDARG00000018902.
DR GeneID; 30228; -.
DR KEGG; dre:30228; -.
DR CTD; 30228; -.
DR ZFIN; ZDB-GENE-990415-206; pes.
DR eggNOG; KOG2481; Eukaryota.
DR GeneTree; ENSGT00390000002626; -.
DR HOGENOM; CLU_019619_0_0_1; -.
DR InParanoid; P79741; -.
DR OMA; TWIVPHY; -.
DR OrthoDB; 777920at2759; -.
DR PhylomeDB; P79741; -.
DR TreeFam; TF300671; -.
DR Reactome; R-DRE-6791226; Major pathway of rRNA processing in the nucleolus and cytosol.
DR PRO; PR:P79741; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 5.
DR Bgee; ENSDARG00000018902; Expressed in gastrula and 47 other tissues.
DR GO; GO:0005730; C:nucleolus; ISS:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; ISS:UniProtKB.
DR GO; GO:0070545; C:PeBoW complex; ISS:UniProtKB.
DR GO; GO:0030687; C:preribosome, large subunit precursor; ISS:UniProtKB.
DR GO; GO:0043021; F:ribonucleoprotein complex binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0031017; P:exocrine pancreas development; IMP:ZFIN.
DR GO; GO:0000466; P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); ISS:UniProtKB.
DR GO; GO:0000463; P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); ISS:UniProtKB.
DR GO; GO:0048709; P:oligodendrocyte differentiation; IMP:ZFIN.
DR GO; GO:0051726; P:regulation of cell cycle; IMP:ZFIN.
DR Gene3D; 3.40.50.10190; -; 1.
DR HAMAP; MF_03028; Pescadillo; 1.
DR InterPro; IPR001357; BRCT_dom.
DR InterPro; IPR036420; BRCT_dom_sf.
DR InterPro; IPR010613; PES.
DR PANTHER; PTHR12221; PTHR12221; 1.
DR Pfam; PF16589; BRCT_2; 1.
DR Pfam; PF06732; Pescadillo_N; 1.
DR SMART; SM00292; BRCT; 1.
DR SUPFAM; SSF52113; SSF52113; 1.
DR PROSITE; PS50172; BRCT; 1.
PE 2: Evidence at transcript level;
KW Coiled coil; Nucleus; Reference proteome; Ribosome biogenesis;
KW rRNA processing.
FT CHAIN 1..583
FT /note="Pescadillo"
FT /id="PRO_0000186190"
FT DOMAIN 323..416
FT /note="BRCT"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03028"
FT REGION 448..526
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 558..583
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 275..329
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03028"
FT COMPBIAS 454..489
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 512..526
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 558..576
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 49
FT /note="K -> R (in Ref. 3; AAH49339)"
FT /evidence="ECO:0000305"
FT CONFLICT 172
FT /note="I -> L (in Ref. 3; AAI60624)"
FT /evidence="ECO:0000305"
FT CONFLICT 199
FT /note="I -> V (in Ref. 3; AAI60624)"
FT /evidence="ECO:0000305"
FT CONFLICT 574
FT /note="A -> E (in Ref. 3; AAH49339)"
FT /evidence="ECO:0000305"
FT CONFLICT 576
FT /note="Missing (in Ref. 1; AAB61138 and 3; AAH49339/
FT AAI60624)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 583 AA; 67898 MW; 454C6D59F83DFEBF CRC64;
MGGLQKKKYE SGSATNYITR NKARKKLSLS LADFRRLCIL KGIYPHEPKH KKKVNKGSTA
ARTYYLLKDI RFLLHEPIVG KFREYKIFVR KLRKAYGKAE WSAVERLKEN KPGYKLDHII
KERYPTFIDA LRDVDDALSM CFLFSTFART GKCHVQTIQL CRRLSVEWMN YIISSRSLRK
VFLSIKGIYY QAEVLGQTIT WIVPYQFAHN HPTDVDYRVM ATFTELYTTL LGFVNFRLYQ
TLNLVYPPKL DGQGEISLKA EFEEDYALES ESYTEKLSAL SASLARMVAS VEEEEAELDH
FPTEGEDQEK MEVREKMEQQ QSKQKKLFEG LKFFLNREVP RESLAFVIRC FGGEVSWDKS
LCIGSTYEAT DETITHHIVD RPSMDKQYIN RYYIQPQWVY DSVNAKIQLP VEEYFLGVTL
PPHLSPFVEE TEGDYVPPEK LKLMALQRGE KPQAEEDEEE EGEEEEDDEE DEEDDEQSED
EEEAEEEANL AEMEEKRSQG KSLSVKVTPG KAKAENRARA AEEEKAEEKR LAIMMMKKKE
KYLYDKIMFG KKRKVREANK LAAKRKAHDD ASKADKKKKK KKC
