PET18_YEAST
ID PET18_YEAST Reviewed; 215 AA.
AC P25362; D6VR28;
DT 01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1992, sequence version 1.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Protein PET18;
GN Name=PET18; Synonyms=HIT2; OrderedLocusNames=YCR020C; ORFNames=YCR20C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3916862; DOI=10.1002/yea.320010204;
RA Toh-e A., Sahashi Y.;
RT "The PET18 locus of Saccharomyces cerevisiae: a complex locus containing
RT multiple genes.";
RL Yeast 1:159-171(1985).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=1574125; DOI=10.1038/357038a0;
RA Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M.,
RA Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G.,
RA Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A.,
RA Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M., Carcano C.,
RA Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M.,
RA Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C.,
RA Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F.,
RA Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C.,
RA Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E.,
RA Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P.,
RA Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J.,
RA Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P.,
RA Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M.,
RA Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P.,
RA Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G.,
RA Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E.,
RA Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F.,
RA Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L.,
RA Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J.,
RA Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M.,
RA Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A.,
RA Richterich P., Roberts A.B., Rodriguez F., Sanz E.,
RA Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J.,
RA Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I.,
RA Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M.,
RA Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M.,
RA Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D.,
RA Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K.,
RA Sgouros J.G.;
RT "The complete DNA sequence of yeast chromosome III.";
RL Nature 357:38-46(1992).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- MISCELLANEOUS: Present with 195 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the thiaminase-2 family. {ECO:0000305}.
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DR EMBL; X59720; CAA42311.1; -; Genomic_DNA.
DR EMBL; AY692952; AAT92971.1; -; Genomic_DNA.
DR EMBL; BK006937; DAA07497.1; -; Genomic_DNA.
DR PIR; S19431; S19431.
DR RefSeq; NP_009947.1; NM_001178733.1.
DR AlphaFoldDB; P25362; -.
DR SMR; P25362; -.
DR BioGRID; 31001; 73.
DR IntAct; P25362; 1.
DR STRING; 4932.YCR020C; -.
DR PaxDb; P25362; -.
DR PRIDE; P25362; -.
DR EnsemblFungi; YCR020C_mRNA; YCR020C; YCR020C.
DR GeneID; 850382; -.
DR KEGG; sce:YCR020C; -.
DR SGD; S000000613; PET18.
DR VEuPathDB; FungiDB:YCR020C; -.
DR eggNOG; ENOG502QT8P; Eukaryota.
DR HOGENOM; CLU_077537_0_0_1; -.
DR InParanoid; P25362; -.
DR OMA; AEWITLH; -.
DR BioCyc; YEAST:G3O-29335-MON; -.
DR PRO; PR:P25362; -.
DR Proteomes; UP000002311; Chromosome III.
DR RNAct; P25362; protein.
DR GO; GO:0005829; C:cytosol; IDA:SGD.
DR GO; GO:0006772; P:thiamine metabolic process; IEP:SGD.
DR Gene3D; 1.20.910.10; -; 1.
DR InterPro; IPR016084; Haem_Oase-like_multi-hlx.
DR InterPro; IPR026285; TenA_E.
DR InterPro; IPR004305; Thiaminase-2/PQQC.
DR Pfam; PF03070; TENA_THI-4; 1.
DR PIRSF; PIRSF003170; Pet18p; 1.
DR SUPFAM; SSF48613; SSF48613; 1.
PE 1: Evidence at protein level;
KW Reference proteome.
FT CHAIN 1..215
FT /note="Protein PET18"
FT /id="PRO_0000192043"
FT CONFLICT 40
FT /note="I -> M (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 104
FT /note="D -> G (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 122
FT /note="D -> S (in Ref. 1)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 215 AA; 25224 MW; 71A2D7C5695CEA13 CRC64;
MSCTTDKLIQ KYDALVRKTT EHKFAKELCA GTLKDRSLYI YLSQDLQFFE TSLRLICKTT
SLAPTTHALI TLAKKIGFFS NDENSYFHDC LELLAPSLTK EERDNFDNKA IPGVDAYINF
LDELRKDASI TWPSLVTSLW VAEELYWRWA RDTPRAPGLH WKYQKWIDLH DGEHFQTWCE
FLKAEVDKFP VEEVESIFVK VSQFEFEFFE SCYNA
