PET8_YEAST
ID PET8_YEAST Reviewed; 284 AA.
AC P38921; D6W1H4;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 171.
DE RecName: Full=Putative mitochondrial carrier protein PET8;
GN Name=PET8; OrderedLocusNames=YNL003C; ORFNames=N2012;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=S288c / GRF88;
RX PubMed=8402262;
RA Lalo D., Stettler S., Mariotte S., Slonimski P.P., Thuriaux P.;
RT "Two yeast chromosomes are related by a fossil duplication of their
RT centromeric regions.";
RL C. R. Acad. Sci. III, Sci. Vie 316:367-373(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=S288c / GRF88;
RX PubMed=7941739; DOI=10.1002/yea.320100412;
RA Lalo D., Stettler S., Mariotte S., Gendreau E., Thuriaux P.;
RT "Organization of the centromeric region of chromosome XIV in Saccharomyces
RT cerevisiae.";
RL Yeast 10:523-533(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 96604 / S288c / FY1679;
RX PubMed=7985421; DOI=10.1002/yea.320100709;
RA Verhasselt P., Aert R., Voet M., Volckaert G.;
RT "Nucleotide sequence analysis of an 8887 bp region of the left arm of yeast
RT chromosome XIV, encompassing the centromere sequence.";
RL Yeast 10:945-951(1994).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169873;
RA Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K.,
RA Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K.,
RA Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M.,
RA Beinhauer J.D., Boskovic J., Buitrago M.J., Bussereau F., Coster F.,
RA Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F.,
RA Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C.,
RA Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A.,
RA Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H.,
RA Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L.,
RA Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R.,
RA Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D.,
RA Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A.,
RA Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C.,
RA Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F.,
RA Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G.,
RA Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M.,
RA Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its
RT evolutionary implications.";
RL Nature 387:93-98(1997).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000305};
CC Multi-pass membrane protein {ECO:0000305}.
CC -!- MISCELLANEOUS: Present with 521 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the mitochondrial carrier (TC 2.A.29) family.
CC {ECO:0000305}.
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DR EMBL; U02536; AAA64802.1; -; Genomic_DNA.
DR EMBL; X77114; CAA54377.1; -; Genomic_DNA.
DR EMBL; Z71279; CAA95862.1; -; Genomic_DNA.
DR EMBL; BK006947; DAA10540.1; -; Genomic_DNA.
DR PIR; S45458; S45458.
DR RefSeq; NP_014395.3; NM_001182842.3.
DR AlphaFoldDB; P38921; -.
DR SMR; P38921; -.
DR BioGRID; 35822; 453.
DR DIP; DIP-2944N; -.
DR IntAct; P38921; 2.
DR MINT; P38921; -.
DR STRING; 4932.YNL003C; -.
DR TCDB; 2.A.29.18.1; the mitochondrial carrier (mc) family.
DR MaxQB; P38921; -.
DR PaxDb; P38921; -.
DR PRIDE; P38921; -.
DR EnsemblFungi; YNL003C_mRNA; YNL003C; YNL003C.
DR GeneID; 855729; -.
DR KEGG; sce:YNL003C; -.
DR SGD; S000004948; PET8.
DR VEuPathDB; FungiDB:YNL003C; -.
DR eggNOG; KOG0768; Eukaryota.
DR GeneTree; ENSGT00550000074950; -.
DR HOGENOM; CLU_015166_3_0_1; -.
DR InParanoid; P38921; -.
DR OMA; PRVGWIS; -.
DR BioCyc; YEAST:G3O-33045-MON; -.
DR Reactome; R-SCE-425393; Transport of inorganic cations/anions and amino acids/oligopeptides.
DR PRO; PR:P38921; -.
DR Proteomes; UP000002311; Chromosome XIV.
DR RNAct; P38921; protein.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005743; C:mitochondrial inner membrane; IBA:GO_Central.
DR GO; GO:0005739; C:mitochondrion; IDA:SGD.
DR GO; GO:0000095; F:S-adenosyl-L-methionine transmembrane transporter activity; IDA:SGD.
DR GO; GO:0015805; P:S-adenosyl-L-methionine transport; IDA:SGD.
DR Gene3D; 1.50.40.10; -; 1.
DR InterPro; IPR018108; Mitochondrial_sb/sol_carrier.
DR InterPro; IPR023395; Mt_carrier_dom_sf.
DR Pfam; PF00153; Mito_carr; 3.
DR SUPFAM; SSF103506; SSF103506; 1.
DR PROSITE; PS50920; SOLCAR; 3.
PE 1: Evidence at protein level;
KW Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW Repeat; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..284
FT /note="Putative mitochondrial carrier protein PET8"
FT /id="PRO_0000090686"
FT TRANSMEM 5..25
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 50..70
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 98..118
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 153..169
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 194..214
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 252..272
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT REPEAT 2..75
FT /note="Solcar 1"
FT REPEAT 92..178
FT /note="Solcar 2"
FT REPEAT 192..271
FT /note="Solcar 3"
SQ SEQUENCE 284 AA; 31027 MW; 761378D67DAFD343 CRC64;
MNTFFLSLLS GAAAGTSTDL VFFPIDTIKT RLQAKGGFFA NGGYKGIYRG LGSAVVASAP
GASLFFISYD YMKVKSRPYI SKLYSQGSEQ LIDTTTHMLS SSIGEICACL VRVPAEVVKQ
RTQVHSTNSS WQTLQSILRN DNKEGLRKNL YRGWSTTIMR EIPFTCIQFP LYEYLKKTWA
KANGQSQVEP WKGAICGSIA GGIAAATTTP LDFLKTRLML NKTTASLGSV IIRIYREEGP
AVFFSGVGPR TMWISAGGAI FLGMYETVHS LLSKSFPTAG EMRA
