PETD_CHLRE
ID PETD_CHLRE Reviewed; 160 AA.
AC P23230; B7U1E5;
DT 01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1991, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Cytochrome b6-f complex subunit 4 {ECO:0000255|HAMAP-Rule:MF_01344};
DE AltName: Full=17 kDa polypeptide {ECO:0000255|HAMAP-Rule:MF_01344};
GN Name=petD {ECO:0000255|HAMAP-Rule:MF_01344};
OS Chlamydomonas reinhardtii (Chlamydomonas smithii).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX NCBI_TaxID=3055;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=2137;
RX PubMed=2017378; DOI=10.1093/nar/19.4.957;
RA Yu W., Spreitzer R.J.;
RT "Sequences of trnR-ACG and petD that contain a tRNA-like element within the
RT chloroplast genome of Chlamydomonas reinhardtii.";
RL Nucleic Acids Res. 19:957-957(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=137c / CC-125;
RX PubMed=2060646; DOI=10.1016/0014-5793(91)80698-3;
RA Bueschlen S., Choquet Y., Kuras R., Wollman F.A.;
RT "Nucleotide sequences of the continuous and separated petA, petB and petD
RT chloroplast genes in Chlamydomonas reinhardtii.";
RL FEBS Lett. 284:257-262(1991).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CC-503;
RX PubMed=19473533; DOI=10.1186/1471-2148-9-120;
RA Smith D.R., Lee R.W.;
RT "Nucleotide diversity of the Chlamydomonas reinhardtii plastid genome:
RT addressing the mutational-hazard hypothesis.";
RL BMC Evol. Biol. 9:120-120(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-8.
RC STRAIN=CC-400;
RX PubMed=8421681; DOI=10.1073/pnas.90.2.497;
RA Sakamoto W., Kindle K.L., Stern D.B.;
RT "In vivo analysis of Chlamydomonas chloroplast petD gene expression using
RT stable transformation of beta-glucuronidase translational fusions.";
RL Proc. Natl. Acad. Sci. U.S.A. 90:497-501(1993).
RN [5]
RP IDENTIFICATION, AND COMPLETE PLASTID GENOME.
RX PubMed=12417694; DOI=10.1105/tpc.006155;
RA Maul J.E., Lilly J.W., Cui L., dePamphilis C.W., Miller W., Harris E.H.,
RA Stern D.B.;
RT "The Chlamydomonas reinhardtii plastid chromosome: islands of genes in a
RT sea of repeats.";
RL Plant Cell 14:2659-2679(2002).
RN [6]
RP CHARACTERIZATION.
RC STRAIN=WT12;
RX PubMed=7493968; DOI=10.1074/jbc.270.49.29342;
RA Pierre Y., Breyton C., Kramer D., Popot J.-L.;
RT "Purification and characterization of the cytochrome b6 f complex from
RT Chlamydomonas reinhardtii.";
RL J. Biol. Chem. 270:29342-29349(1995).
RN [7]
RP X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS) OF 1-159.
RX PubMed=14647374; DOI=10.1038/nature02155;
RA Stroebel D., Choquet Y., Popot J.-L., Picot D.;
RT "An atypical haem in the cytochrome b(6)f complex.";
RL Nature 426:413-418(2003).
CC -!- FUNCTION: Component of the cytochrome b6-f complex, which mediates
CC electron transfer between photosystem II (PSII) and photosystem I
CC (PSI), cyclic electron flow around PSI, and state transitions.
CC -!- SUBUNIT: The 4 large subunits of the cytochrome b6-f complex are
CC cytochrome b6, subunit IV (17 kDa polypeptide, petD), cytochrome f and
CC the Rieske protein, while the 4 small subunits are petG, petL, petM and
CC petN. The complex functions as a dimer.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane; Multi-
CC pass membrane protein.
CC -!- PTM: The N-terminus is blocked.
CC -!- SIMILARITY: Belongs to the cytochrome b family. PetD subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_01344}.
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DR EMBL; X56700; CAA40030.1; -; Genomic_DNA.
DR EMBL; X72919; CAA51424.1; -; Genomic_DNA.
DR EMBL; FJ423446; ACJ50092.1; -; Genomic_DNA.
DR EMBL; L05506; AAA84153.1; -; Genomic_DNA.
DR EMBL; BK000554; DAA00905.1; -; Genomic_DNA.
DR PIR; S16918; S16918.
DR RefSeq; NP_958359.1; NC_005353.1.
DR PDB; 1Q90; X-ray; 3.10 A; D=1-159.
DR PDBsum; 1Q90; -.
DR AlphaFoldDB; P23230; -.
DR SMR; P23230; -.
DR IntAct; P23230; 1.
DR STRING; 3055.DAA00905; -.
DR PaxDb; P23230; -.
DR PRIDE; P23230; -.
DR GeneID; 2717021; -.
DR KEGG; cre:ChreCp002; -.
DR eggNOG; KOG4663; Eukaryota.
DR HOGENOM; CLU_112652_0_0_1; -.
DR InParanoid; P23230; -.
DR OrthoDB; 1125966at2759; -.
DR BioCyc; MetaCyc:CHRECP002-MON; -.
DR EvolutionaryTrace; P23230; -.
DR Proteomes; UP000006906; Chloroplast.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0045158; F:electron transporter, transferring electrons within cytochrome b6/f complex of photosystem II activity; IEA:UniProtKB-UniRule.
DR GO; GO:0045156; F:electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity; IEA:InterPro.
DR GO; GO:0009767; P:photosynthetic electron transport chain; IEA:InterPro.
DR CDD; cd00290; cytochrome_b_C; 1.
DR HAMAP; MF_01344; Cytb6_f_subIV; 1.
DR InterPro; IPR005798; Cyt_b/b6_C.
DR InterPro; IPR036150; Cyt_b/b6_C_sf.
DR InterPro; IPR005870; Cyt_b6/f_cplx_suIV.
DR Pfam; PF00032; Cytochrom_B_C; 1.
DR PIRSF; PIRSF000033; B6f_17K; 1.
DR SUPFAM; SSF81648; SSF81648; 1.
DR TIGRFAMs; TIGR01156; cytb6/f_IV; 1.
DR PROSITE; PS51003; CYTB_CTER; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chloroplast; Electron transport; Membrane; Photosynthesis;
KW Plastid; Reference proteome; Thylakoid; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..160
FT /note="Cytochrome b6-f complex subunit 4"
FT /id="PRO_0000061852"
FT TRANSMEM 36..56
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01344"
FT TRANSMEM 95..115
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01344"
FT TRANSMEM 131..151
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01344"
FT CONFLICT 67
FT /note="N -> F (in Ref. 2)"
FT /evidence="ECO:0000305"
FT HELIX 12..20
FT /evidence="ECO:0007829|PDB:1Q90"
FT HELIX 24..26
FT /evidence="ECO:0007829|PDB:1Q90"
FT STRAND 27..30
FT /evidence="ECO:0007829|PDB:1Q90"
FT HELIX 34..38
FT /evidence="ECO:0007829|PDB:1Q90"
FT HELIX 39..57
FT /evidence="ECO:0007829|PDB:1Q90"
FT HELIX 79..81
FT /evidence="ECO:0007829|PDB:1Q90"
FT HELIX 82..90
FT /evidence="ECO:0007829|PDB:1Q90"
FT HELIX 94..108
FT /evidence="ECO:0007829|PDB:1Q90"
FT TURN 109..115
FT /evidence="ECO:0007829|PDB:1Q90"
FT HELIX 123..125
FT /evidence="ECO:0007829|PDB:1Q90"
FT HELIX 127..146
FT /evidence="ECO:0007829|PDB:1Q90"
FT HELIX 151..154
FT /evidence="ECO:0007829|PDB:1Q90"
SQ SEQUENCE 160 AA; 17442 MW; 6B1C77BBB8E857A8 CRC64;
MSVTKKPDLS DPVLKAKLAK GMGHNTYGEP AWPNDLLYMF PVVILGTFAC VIGLSVLDPA
AMGEPANPFA TPLEILPEWY FYPVFQILRV VPNKLLGVLL MAAVPAGLIT VPFIESINKF
QNPYRRPIAT ILFLLGTLVA VWLGIGSTFP IDISLTLGLF
