PETG_CHLRE
ID PETG_CHLRE Reviewed; 37 AA.
AC Q08362; B7U1I5;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Cytochrome b6-f complex subunit 5 {ECO:0000255|HAMAP-Rule:MF_00432};
DE AltName: Full=Cytochrome b6-f complex subunit PetG {ECO:0000255|HAMAP-Rule:MF_00432};
DE AltName: Full=Cytochrome b6-f complex subunit V {ECO:0000255|HAMAP-Rule:MF_00432};
GN Name=petG {ECO:0000255|HAMAP-Rule:MF_00432};
OS Chlamydomonas reinhardtii (Chlamydomonas smithii).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX NCBI_TaxID=3055;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1617741; DOI=10.1007/bf00351664;
RA Fong S.E., Surzycki S.J.;
RT "Organization and structure of plastome psbF, psbL, petG and ORF712 genes
RT in Chlamydomonas reinhardtii.";
RL Curr. Genet. 21:527-530(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND CHARACTERIZATION.
RC STRAIN=137c / CC-125;
RX PubMed=7493961; DOI=10.1074/jbc.270.49.29293;
RA Berthold D.A., Schmidt C.L., Malkin R.;
RT "The deletion of petG in Chlamydomonas reinhardtii disrupts the cytochrome
RT bf complex.";
RL J. Biol. Chem. 270:29293-29298(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CC-503;
RX PubMed=19473533; DOI=10.1186/1471-2148-9-120;
RA Smith D.R., Lee R.W.;
RT "Nucleotide diversity of the Chlamydomonas reinhardtii plastid genome:
RT addressing the mutational-hazard hypothesis.";
RL BMC Evol. Biol. 9:120-120(2009).
RN [4]
RP PROTEIN SEQUENCE OF 1-15.
RC STRAIN=CC-124;
RA Schmidt C.L., Malkin R.;
RT "Low molecular weight subunits associated with the cytochrome b6f complexes
RT from spinach and Chlamydomonas reinhardtii.";
RL Photosyn. Res. 38:73-81(1993).
RN [5]
RP IDENTIFICATION, AND COMPLETE PLASTID GENOME.
RX PubMed=12417694; DOI=10.1105/tpc.006155;
RA Maul J.E., Lilly J.W., Cui L., dePamphilis C.W., Miller W., Harris E.H.,
RA Stern D.B.;
RT "The Chlamydomonas reinhardtii plastid chromosome: islands of genes in a
RT sea of repeats.";
RL Plant Cell 14:2659-2679(2002).
RN [6]
RP CHARACTERIZATION.
RC STRAIN=WT12;
RX PubMed=7493968; DOI=10.1074/jbc.270.49.29342;
RA Pierre Y., Breyton C., Kramer D., Popot J.-L.;
RT "Purification and characterization of the cytochrome b6 f complex from
RT Chlamydomonas reinhardtii.";
RL J. Biol. Chem. 270:29342-29349(1995).
RN [7]
RP X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS) OF 1-37.
RX PubMed=14647374; DOI=10.1038/nature02155;
RA Stroebel D., Choquet Y., Popot J.-L., Picot D.;
RT "An atypical haem in the cytochrome b(6)f complex.";
RL Nature 426:413-418(2003).
CC -!- FUNCTION: Component of the cytochrome b6-f complex, which mediates
CC electron transfer between photosystem II (PSII) and photosystem I
CC (PSI), cyclic electron flow around PSI, and state transitions. PetG is
CC required for either the stability or assembly of the cytochrome b6-f
CC complex. {ECO:0000255|HAMAP-Rule:MF_00432}.
CC -!- SUBUNIT: The 4 large subunits of the cytochrome b6-f complex are
CC cytochrome b6, subunit IV (17 kDa polypeptide, PetD), cytochrome f and
CC the Rieske protein, while the 4 small subunits are PetG, PetL, PetM and
CC PetN. The complex functions as a dimer.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane; Single-
CC pass membrane protein.
CC -!- SIMILARITY: Belongs to the PetG family. {ECO:0000255|HAMAP-
CC Rule:MF_00432}.
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DR EMBL; X66250; CAA46979.1; -; Genomic_DNA.
DR EMBL; FJ423446; ACJ50132.1; -; Genomic_DNA.
DR EMBL; BK000554; DAA00946.1; -; Genomic_DNA.
DR PIR; S26880; S26880.
DR RefSeq; NP_958401.1; NC_005353.1.
DR PDB; 1Q90; X-ray; 3.10 A; G=1-37.
DR PDBsum; 1Q90; -.
DR AlphaFoldDB; Q08362; -.
DR SMR; Q08362; -.
DR IntAct; Q08362; 1.
DR STRING; 3055.DAA00946; -.
DR PaxDb; Q08362; -.
DR GeneID; 2716996; -.
DR KEGG; cre:ChreCp045; -.
DR eggNOG; ENOG502SD3G; Eukaryota.
DR HOGENOM; CLU_216962_0_0_1; -.
DR InParanoid; Q08362; -.
DR OrthoDB; 1641963at2759; -.
DR BioCyc; MetaCyc:CHRECP045-MON; -.
DR EvolutionaryTrace; Q08362; -.
DR Proteomes; UP000006906; Chloroplast.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009512; C:cytochrome b6f complex; IEA:InterPro.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0045158; F:electron transporter, transferring electrons within cytochrome b6/f complex of photosystem II activity; IEA:UniProtKB-UniRule.
DR GO; GO:0017004; P:cytochrome complex assembly; IEA:UniProtKB-UniRule.
DR GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR HAMAP; MF_00432; Cytb6_f_PetG; 1.
DR InterPro; IPR003683; Cyt_6/f_cplx_su5.
DR InterPro; IPR036099; Cyt_6/f_cplx_su5_sf.
DR PANTHER; PTHR35516; PTHR35516; 1.
DR Pfam; PF02529; PetG; 1.
DR PIRSF; PIRSF000034; Cyt_b6-f_V; 1.
DR SUPFAM; SSF103446; SSF103446; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chloroplast; Direct protein sequencing; Electron transport;
KW Membrane; Photosynthesis; Plastid; Reference proteome; Thylakoid;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..37
FT /note="Cytochrome b6-f complex subunit 5"
FT /id="PRO_0000216376"
FT TRANSMEM 5..25
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00432"
FT VARIANT 30
FT /note="L -> R (in strain: CC-503)"
FT CONFLICT 7
FT /note="C -> L (in Ref. 4; AA sequence)"
FT /evidence="ECO:0000305"
FT HELIX 4..29
FT /evidence="ECO:0007829|PDB:1Q90"
SQ SEQUENCE 37 AA; 3984 MW; AC71B49852247A40 CRC64;
MVEPLLCGIV LGLVPVTIAG LFVTAYLQYL RGDLATY