PETL_CHLRE
ID PETL_CHLRE Reviewed; 43 AA.
AC P50369; B7U1K8;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 11-JUL-2003, sequence version 3.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=Cytochrome b6-f complex subunit 6 {ECO:0000255|HAMAP-Rule:MF_00433};
DE AltName: Full=Cytochrome b6-f complex subunit PetL {ECO:0000255|HAMAP-Rule:MF_00433};
DE AltName: Full=Cytochrome b6-f complex subunit VI {ECO:0000255|HAMAP-Rule:MF_00433};
GN Name=petL {ECO:0000255|HAMAP-Rule:MF_00433};
OS Chlamydomonas reinhardtii (Chlamydomonas smithii).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX NCBI_TaxID=3055;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=137c / CC-125;
RX PubMed=8670852; DOI=10.1002/j.1460-2075.1996.tb00718.x;
RA Takahashi Y., Rahire M., Breyton C., Popot J.-L., Joliot P., Rochaix J.-D.;
RT "The chloroplast ycf7 (petL) open reading frame of Chlamydomonas
RT reinhardtii encodes a small functionally important subunit of the
RT cytochrome b6f complex.";
RL EMBO J. 15:3498-3506(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CC-503;
RX PubMed=19473533; DOI=10.1186/1471-2148-9-120;
RA Smith D.R., Lee R.W.;
RT "Nucleotide diversity of the Chlamydomonas reinhardtii plastid genome:
RT addressing the mutational-hazard hypothesis.";
RL BMC Evol. Biol. 9:120-120(2009).
RN [3]
RP IDENTIFICATION, AND COMPLETE PLASTID GENOME.
RX PubMed=12417694; DOI=10.1105/tpc.006155;
RA Maul J.E., Lilly J.W., Cui L., dePamphilis C.W., Miller W., Harris E.H.,
RA Stern D.B.;
RT "The Chlamydomonas reinhardtii plastid chromosome: islands of genes in a
RT sea of repeats.";
RL Plant Cell 14:2659-2679(2002).
RN [4]
RP CHARACTERIZATION.
RC STRAIN=WT12;
RX PubMed=7493968; DOI=10.1074/jbc.270.49.29342;
RA Pierre Y., Breyton C., Kramer D., Popot J.-L.;
RT "Purification and characterization of the cytochrome b6 f complex from
RT Chlamydomonas reinhardtii.";
RL J. Biol. Chem. 270:29342-29349(1995).
RN [5]
RP IDENTIFICATION OF START CODON, AND FUSION WITH SUBUNIT IV (PETD).
RC STRAIN=137c / CC-125;
RX PubMed=11796719; DOI=10.1074/jbc.m110914200;
RA Zito F., Vinh J., Popot J.-L., Finazzi G.;
RT "Chimeric fusions of subunit IV and PetL in the b6f complex of
RT Chlamydomonas reinhardtii: structural implications and consequences on
RT state transitions.";
RL J. Biol. Chem. 277:12446-12455(2002).
RN [6]
RP X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS) OF 12-43.
RX PubMed=14647374; DOI=10.1038/nature02155;
RA Stroebel D., Choquet Y., Popot J.-L., Picot D.;
RT "An atypical haem in the cytochrome b(6)f complex.";
RL Nature 426:413-418(2003).
CC -!- FUNCTION: Component of the cytochrome b6-f complex, which mediates
CC electron transfer between photosystem II (PSII) and photosystem I
CC (PSI), cyclic electron flow around PSI, and state transitions. PetL is
CC important for photoautotrophic growth as well as for electron transfer
CC efficiency and stability of the cytochrome b6-f complex.
CC {ECO:0000255|HAMAP-Rule:MF_00433}.
CC -!- SUBUNIT: The 4 large subunits of the cytochrome b6-f complex are
CC cytochrome b6, subunit IV (17 kDa polypeptide, PetD), cytochrome f and
CC the Rieske protein, while the 4 small subunits are PetG, PetL, PetM and
CC PetN. The complex functions as a dimer. {ECO:0000255|HAMAP-
CC Rule:MF_00433}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000255|HAMAP-Rule:MF_00433}; Single-pass membrane protein
CC {ECO:0000255|HAMAP-Rule:MF_00433}.
CC -!- PTM: The N-terminus is blocked.
CC -!- MISCELLANEOUS: A deletion of this gene can be substituted for by a
CC subunit IV-petL fusion. The fusion protein does not permit state
CC transitions, although all other functions are wild-type.
CC -!- SIMILARITY: Belongs to the PetL family. {ECO:0000255|HAMAP-
CC Rule:MF_00433}.
CC -!- CAUTION: Mass spectrometry indicates that the start site is probably
CC Met-1, and not Met-12 as is thought to be the case in other organisms.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ACJ50155.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; U43964; AAB17716.1; -; Genomic_DNA.
DR EMBL; FJ423446; ACJ50155.1; ALT_INIT; Genomic_DNA.
DR EMBL; BK000554; DAA00968.1; -; Genomic_DNA.
DR PIR; T08024; T08024.
DR RefSeq; NP_958424.2; NC_005353.1.
DR PDB; 1Q90; X-ray; 3.10 A; L=12-43.
DR PDBsum; 1Q90; -.
DR AlphaFoldDB; P50369; -.
DR SMR; P50369; -.
DR IntAct; P50369; 1.
DR STRING; 3055.DAA00968; -.
DR PaxDb; P50369; -.
DR GeneID; 2717047; -.
DR KEGG; cre:ChreCp068; -.
DR HOGENOM; CLU_3242873_0_0_1; -.
DR BioCyc; MetaCyc:CHRECP068-MON; -.
DR EvolutionaryTrace; P50369; -.
DR Proteomes; UP000006906; Chloroplast.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009512; C:cytochrome b6f complex; IEA:InterPro.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0045158; F:electron transporter, transferring electrons within cytochrome b6/f complex of photosystem II activity; IEA:UniProtKB-UniRule.
DR GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR HAMAP; MF_00433; Cytb6_f_PetL; 1.
DR InterPro; IPR007802; Cyt_b6/f_cplx_su6.
DR Pfam; PF05115; PetL; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chloroplast; Electron transport; Membrane; Photosynthesis;
KW Plastid; Reference proteome; Thylakoid; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..43
FT /note="Cytochrome b6-f complex subunit 6"
FT /id="PRO_0000220445"
FT TRANSMEM 17..37
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00433"
FT HELIX 13..37
FT /evidence="ECO:0007829|PDB:1Q90"
SQ SEQUENCE 43 AA; 4878 MW; B7E6977ED788C9F3 CRC64;
MIFDFNYIHI FMLTITSYVG LLIGALVFTL GIYLGLLKVV KLI
