PETM_CHLRE
ID PETM_CHLRE Reviewed; 99 AA.
AC Q42496;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 95.
DE RecName: Full=Cytochrome b6-f complex subunit 7, chloroplastic;
DE AltName: Full=Cytochrome b6-f complex 4 kDa subunit;
DE AltName: Full=Cytochrome b6-f complex subunit PetM;
DE AltName: Full=Cytochrome b6-f complex subunit VII;
DE Flags: Precursor;
GN Name=petM; Synonyms=petX;
OS Chlamydomonas reinhardtii (Chlamydomonas smithii).
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX NCBI_TaxID=3055;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 61-98.
RC STRAIN=cw15;
RX PubMed=8631873; DOI=10.1074/jbc.271.18.10667;
RA de Vitry C., Breyton C., Pierre Y., Popot J.-L.;
RT "The 4-kDa nuclear-encoded PetM polypeptide of the chloroplast cytochrome
RT b6f complex. Nucleic acid and protein sequences, targeting signals,
RT transmembrane topology.";
RL J. Biol. Chem. 271:10667-10671(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=CC-1928;
RX PubMed=8616155; DOI=10.1016/0005-2728(95)00157-3;
RA Ketchner S.L., Malkin R.;
RT "Nucleotide sequence of the PetM gene encoding a 4 kDa subunit of the
RT cytochrome b6f complex from Chlamydomonas reinhardtii.";
RL Biochim. Biophys. Acta 1273:195-197(1996).
RN [3]
RP PROTEIN SEQUENCE OF 61-87, AND CHARACTERIZATION.
RC STRAIN=WT12;
RX PubMed=7493968; DOI=10.1074/jbc.270.49.29342;
RA Pierre Y., Breyton C., Kramer D., Popot J.-L.;
RT "Purification and characterization of the cytochrome b6 f complex from
RT Chlamydomonas reinhardtii.";
RL J. Biol. Chem. 270:29342-29349(1995).
RN [4]
RP PROTEIN SEQUENCE OF 61-82.
RC STRAIN=CC-124;
RA Schmidt C.L., Malkin R.;
RT "Low molecular weight subunits associated with the cytochrome b6f complexes
RT from spinach and Chlamydomonas reinhardtii.";
RL Photosyn. Res. 38:73-81(1993).
RN [5]
RP X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS) OF 61-99.
RX PubMed=14647374; DOI=10.1038/nature02155;
RA Stroebel D., Choquet Y., Popot J.-L., Picot D.;
RT "An atypical haem in the cytochrome b(6)f complex.";
RL Nature 426:413-418(2003).
CC -!- FUNCTION: Component of the cytochrome b6-f complex, which mediates
CC electron transfer between photosystem II (PSII) and photosystem I
CC (PSI), cyclic electron flow around PSI, and state transitions.
CC -!- SUBUNIT: The 4 large subunits of the cytochrome b6-f complex are
CC cytochrome b6, subunit IV (17 kDa polypeptide, PetD), cytochrome f and
CC the Rieske protein, while the 4 small subunits are PetG, PetL, PetM and
CC PetN. The complex functions as a dimer.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane; Single-
CC pass membrane protein.
CC -!- SIMILARITY: Belongs to the PetM family. {ECO:0000305}.
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DR EMBL; X92488; CAA63221.1; -; mRNA.
DR EMBL; U36401; AAC49525.1; -; Genomic_DNA.
DR PIR; T08070; T08070.
DR RefSeq; XP_001694096.1; XM_001694044.1.
DR PDB; 1Q90; X-ray; 3.10 A; M=61-99.
DR PDBsum; 1Q90; -.
DR AlphaFoldDB; Q42496; -.
DR SMR; Q42496; -.
DR IntAct; Q42496; 1.
DR STRING; 3055.EDP03032; -.
DR PRIDE; Q42496; -.
DR EnsemblPlants; PNW76141; PNW76141; CHLRE_12g546150v5.
DR GeneID; 5719581; -.
DR Gramene; PNW76141; PNW76141; CHLRE_12g546150v5.
DR KEGG; cre:CHLRE_12g546150v5; -.
DR eggNOG; ENOG502T01X; Eukaryota.
DR HOGENOM; CLU_2323765_0_0_1; -.
DR OrthoDB; 1619831at2759; -.
DR BioCyc; MetaCyc:CHLREDRAFT_147968-MON; -.
DR EvolutionaryTrace; Q42496; -.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009512; C:cytochrome b6f complex; IEA:InterPro.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR HAMAP; MF_00396; Cytb6_f_PetM; 1.
DR InterPro; IPR012595; PetM_cyt_b6/f_cplx_su7.
DR Pfam; PF08041; PetM; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chloroplast; Direct protein sequencing; Electron transport;
KW Membrane; Plastid; Respiratory chain; Thylakoid; Transit peptide;
KW Transmembrane; Transmembrane helix; Transport.
FT TRANSIT 1..60
FT /note="Chloroplast"
FT /evidence="ECO:0000269|PubMed:7493968,
FT ECO:0000269|PubMed:8631873, ECO:0000269|Ref.4"
FT CHAIN 61..99
FT /note="Cytochrome b6-f complex subunit 7, chloroplastic"
FT /id="PRO_0000023864"
FT TOPO_DOM 61..67
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 68..88
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 89..99
FT /note="Stromal"
FT /evidence="ECO:0000255"
FT HELIX 63..93
FT /evidence="ECO:0007829|PDB:1Q90"
SQ SEQUENCE 99 AA; 9998 MW; 5B3E45315A701AE4 CRC64;
MAMSIAARSA CCGVAAPRSS TVRVAAARPA VRPSLRTAGQ KAAPSRGVAT KAVNELAMIA
GEAEFIAGTA LTMVGMTLVG LAIGFVLLRV ESLVEEGKI
