ID   PEX12_DICDI             Reviewed;         459 AA.
AC   Q54N40;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 97.
DE   RecName: Full=Putative peroxisome assembly protein 12;
DE   AltName: Full=Peroxin-12;
GN   Name=pex12; ORFNames=DDB_G0285523;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Required for protein import into peroxisomes. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Peroxisome membrane {ECO:0000250}; Single-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the pex2/pex10/pex12 family. {ECO:0000305}.
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DR   EMBL; AAFI02000079; EAL64599.1; -; Genomic_DNA.
DR   RefSeq; XP_638103.1; XM_633011.1.
DR   AlphaFoldDB; Q54N40; -.
DR   SMR; Q54N40; -.
DR   STRING; 44689.DDB0238076; -.
DR   PaxDb; Q54N40; -.
DR   EnsemblProtists; EAL64599; EAL64599; DDB_G0285523.
DR   GeneID; 8625151; -.
DR   KEGG; ddi:DDB_G0285523; -.
DR   dictyBase; DDB_G0285523; pex12.
DR   eggNOG; KOG0826; Eukaryota.
DR   HOGENOM; CLU_031067_0_0_1; -.
DR   InParanoid; Q54N40; -.
DR   OMA; VGCYPCL; -.
DR   PhylomeDB; Q54N40; -.
DR   Reactome; R-DDI-8866654; E3 ubiquitin ligases ubiquitinate target proteins.
DR   Reactome; R-DDI-9033241; Peroxisomal protein import.
DR   Reactome; R-DDI-9603798; Class I peroxisomal membrane protein import.
DR   PRO; PR:Q54N40; -.
DR   Proteomes; UP000002195; Chromosome 4.
DR   GO; GO:0005779; C:integral component of peroxisomal membrane; ISS:dictyBase.
DR   GO; GO:1990429; C:peroxisomal importomer complex; IBA:GO_Central.
DR   GO; GO:0008022; F:protein C-terminus binding; IEA:InterPro.
DR   GO; GO:0004842; F:ubiquitin-protein transferase activity; IBA:GO_Central.
DR   GO; GO:0008270; F:zinc ion binding; ISS:dictyBase.
DR   GO; GO:0016558; P:protein import into peroxisome matrix; ISS:dictyBase.
DR   GO; GO:0006513; P:protein monoubiquitination; IBA:GO_Central.
DR   Gene3D; 3.30.40.10; -; 1.
DR   InterPro; IPR017375; PEX12.
DR   InterPro; IPR006845; Pex_N.
DR   InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR   PANTHER; PTHR12888; PTHR12888; 1.
DR   Pfam; PF04757; Pex2_Pex12; 1.
PE   3: Inferred from homology;
KW   Coiled coil; Membrane; Metal-binding; Peroxisome; Reference proteome;
KW   Transmembrane; Transmembrane helix; Zinc; Zinc-finger.
FT   CHAIN           1..459
FT                   /note="Putative peroxisome assembly protein 12"
FT                   /id="PRO_0000372546"
FT   TRANSMEM        266..286
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   ZN_FING         406..444
FT                   /note="RING-type; degenerate"
FT   COILED          196..232
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   459 AA;  53998 MW;  B7F4DC7A40778D2D CRC64;
     MFMFNFDNGH DPNRPSFFEM LNQHQMMPSF KPALKYIFTV LSQRNPKFRY IVNYYDECFY
     SLLLLLEYHY LKYYEGSFSE NFYNLKRIKP RNNNANGDGD TLFSLLKRLV VTPSNAGQGP
     ETYTKSQILK KSFAMIRRNR AAASSSQQAK DDLNTMIQDS DRKESLIYLV LIPYFKGKLD
     EYYKKESDPL AELGLVSSDN NNNNNDNIND QIQQLEEQIQ QQQTIVNGNN NSNNNNKKLK
     IKFLILIRFL KGSKTLKKLK TIFLKVYPFI SAIYEALFFI YQLLYLYEYT NYYTPFFHFQ
     NIQLKRLNHK DIESHRVVIS NRRRDRINFV RDWPGSSFFV RLVSILDSIL DYSKYILPLS
     VFIFKSLEWW YSENRISAPT LPIPTPPTPS KRAPGGLEIP RDKRLCPLCL KERTNPTICG
     SGFVFCYPCI FGYVNEHSKC PITFLPTNTE QLRKIYETV