PEX12_DROME
ID PEX12_DROME Reviewed; 297 AA.
AC Q9VPT5; Q4V3Q4;
DT 24-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=Peroxisome assembly protein 12;
DE AltName: Full=Peroxin-12;
GN Name=Pex12; ORFNames=CG3639;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Celniker S.E.;
RL Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION.
RX PubMed=21669930; DOI=10.1242/dmm.007419;
RA Mast F.D., Li J., Virk M.K., Hughes S.C., Simmonds A.J., Rachubinski R.A.;
RT "A Drosophila model for the Zellweger spectrum of peroxisome biogenesis
RT disorders.";
RL Dis. Model. Mech. 4:659-672(2011).
CC -!- FUNCTION: Required for protein import into peroxisomes. Involved in
CC peroxisome biogenesis. {ECO:0000269|PubMed:21669930}.
CC -!- SUBCELLULAR LOCATION: Peroxisome membrane {ECO:0000305}; Single-pass
CC membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the pex2/pex10/pex12 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE014134; AAF51456.1; -; Genomic_DNA.
DR EMBL; BT023302; AAY55718.1; -; mRNA.
DR RefSeq; NP_001259844.1; NM_001272915.1.
DR RefSeq; NP_608546.1; NM_134702.3.
DR AlphaFoldDB; Q9VPT5; -.
DR SMR; Q9VPT5; -.
DR IntAct; Q9VPT5; 1.
DR STRING; 7227.FBpp0305188; -.
DR PaxDb; Q9VPT5; -.
DR DNASU; 33256; -.
DR EnsemblMetazoa; FBtr0078010; FBpp0077675; FBgn0031282.
DR EnsemblMetazoa; FBtr0332972; FBpp0305188; FBgn0031282.
DR GeneID; 33256; -.
DR KEGG; dme:Dmel_CG3639; -.
DR UCSC; CG3639-RA; d. melanogaster.
DR CTD; 5193; -.
DR FlyBase; FBgn0031282; Pex12.
DR VEuPathDB; VectorBase:FBgn0031282; -.
DR eggNOG; KOG0826; Eukaryota.
DR GeneTree; ENSGT00390000016209; -.
DR HOGENOM; CLU_031067_1_0_1; -.
DR InParanoid; Q9VPT5; -.
DR OMA; VYCWKCI; -.
DR OrthoDB; 1204472at2759; -.
DR PhylomeDB; Q9VPT5; -.
DR Reactome; R-DME-8866654; E3 ubiquitin ligases ubiquitinate target proteins.
DR Reactome; R-DME-9033241; Peroxisomal protein import.
DR Reactome; R-DME-9603798; Class I peroxisomal membrane protein import.
DR SignaLink; Q9VPT5; -.
DR BioGRID-ORCS; 33256; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 33256; -.
DR PRO; PR:Q9VPT5; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0031282; Expressed in crop (Drosophila) and 25 other tissues.
DR ExpressionAtlas; Q9VPT5; baseline and differential.
DR Genevisible; Q9VPT5; DM.
DR GO; GO:0005779; C:integral component of peroxisomal membrane; IBA:GO_Central.
DR GO; GO:1990429; C:peroxisomal importomer complex; IBA:GO_Central.
DR GO; GO:0005777; C:peroxisome; IDA:FlyBase.
DR GO; GO:0008022; F:protein C-terminus binding; IEA:InterPro.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; ISS:FlyBase.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0007031; P:peroxisome organization; IMP:FlyBase.
DR GO; GO:0016558; P:protein import into peroxisome matrix; IBA:GO_Central.
DR GO; GO:0006513; P:protein monoubiquitination; IBA:GO_Central.
DR GO; GO:0007286; P:spermatid development; IMP:FlyBase.
DR GO; GO:0048137; P:spermatocyte division; IMP:FlyBase.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR017375; PEX12.
DR InterPro; IPR006845; Pex_N.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR PANTHER; PTHR12888; PTHR12888; 1.
DR Pfam; PF04757; Pex2_Pex12; 1.
DR PIRSF; PIRSF038074; Peroxisome_assembly_p12; 1.
PE 2: Evidence at transcript level;
KW Membrane; Metal-binding; Peroxisome; Protein transport; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport; Zinc; Zinc-finger.
FT CHAIN 1..297
FT /note="Peroxisome assembly protein 12"
FT /id="PRO_0000218614"
FT TRANSMEM 246..270
FT /note="Helical"
FT /evidence="ECO:0000255"
FT ZN_FING 244..283
FT /note="RING-type; degenerate"
SQ SEQUENCE 297 AA; 34544 MW; 5AB9C7DFE11AF5C0 CRC64;
MAEAANVRQN LQNVPSIFEI SASETLDNLI YPALSKIFDY FGLRLDFKLW GSLRIQEELS
PLLTWLLQYL YLRKRASSFG ESFYGLQRTV TTTGDLLNRR QQFASATLLT FMPYVERKLR
TRITRHEDTS PWEQRLLSAF HAFHAAKAAH TFFYLVKYAS NHSPIFRLLG LTLRYPSEPP
KEDQWTYVVL KMLEVLAFFL QFVQWWYSND QRRKVGGTLI NPEAMPRKQL PKEVQQSLPQ
RGECPVCLLS IQTPTACSVS GYVFCWKCIV SHMKEHGTCP VTHYPISLDD LVRIYET
